Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes

Tomura, Michio; Hata, Akihiko; Matsuoka, Satoshi; Shand, Francis H.W.; Nakanishi, Yasutaka; Ikebuchi, Ryoyo; Ueha, Satoshi; Tomimoto, Hidekazu; Inaba, Kayo; Matsushima, Kouji; Miyawaki, Atsushi; Kabashima, Kenji; Watanabe, Takeshi; Kanagawa, Osami

doi:10.1038/srep06030

Cited by 150 publications

(164 citation statements)

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“…by Langerin + DCs. It is known that in the skin, Langerin + dermal DCs traffic between the skin and peripheral lymph nodes [55]. In the intestine it has not been determined whether Langerin + DCs are migratory.…”

Section: Discussionmentioning

confidence: 99%

Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer’s patches

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer’s patches

et al. 2015

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“…Furthermore, failure of a population to exchange in parabiosis does not imply that cells of that population are sessile and static. For example, in the absence of inflammation, Langerhans cells remain overwhelmingly host-derived in parabiotic mice (Merad et al, 2002), although they migrate continuously and unidirectionally from the epidermis to skin-draining lymph nodes (Bajaña et al, 2012; Ohl et al, 2004; Tomura et al, 2014). Additionally, imaging studies have shown that liver-resident iNKT cells, epidermis-resident CD8 + T RM cells, and intestine-resident ILCs exhibit dynamic behavior within their respective tissues (Gebhardt et al, 2011; Geissmann et al, 2005; Mackley et al, 2015; Pearson et al, 2016; Zaid et al, 2014) but self-renew locally and are not replaced by circulating precursors (Gasteiger et al, 2015; Jiang et al, 2012; Thomas et al, 2011).…”

Section: Definition Of Tissue-resident Populationsmentioning

confidence: 99%

Hallmarks of Tissue-Resident Lymphocytes

Fan

Rudensky

2016

Cell

313

279

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Although they are classically viewed as continuously recirculating through the lymphoid organs and blood, lymphocytes also establish residency in non-lymphoid tissues, most prominently at barrier sites, including the mucosal surfaces and skin. These specialized tissue-resident lymphocyte subsets span the innate-adaptive continuum and include innate lymphoid cells (ILCs), unconventional T cells (e.g., NKT, MAIT, γδ T cells, and CD8αα+IELs), and tissue-resident memory T (TRM) cells. Although these diverse cell types differ in the particulars of their biology, they nonetheless exhibit important shared features, including a role in the preservation of tissue integrity and function during homeostasis, infection, and non-infectious perturbations. In this Review, we discuss the hallmarks of tissue-resident innate, innate-like, and adaptive lymphocytes, as well as their potential functions in non-lymphoid organs.

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“…The reason for this faster turnover is unclear, but it may reflect their more invasive method to surgically expose the SDLNs (30).…”

Section: Dec-205mentioning

confidence: 99%

Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node

Kitano

Yamazaki

Takumi

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

105

108

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Dendritic cells (DCs) are antigen-presenting cells specialized for activating T cells to elicit effector T-cell functions. Cross-presenting DCs are a DC subset capable of presenting antigens to CD8+ T cells and play critical roles in cytotoxic T-cell-mediated immune responses to microorganisms and cancer. Although their importance is known, the spatiotemporal dynamics of cross-presenting DCs in vivo are incompletely understood. Here, we study the T-cell zone in skin-draining lymph nodes (SDLNs) and find it is compartmentalized into regions for CD8 + T-cell activation by cross-presenting DCs that express the chemokine (C motif) receptor 1 gene, Xcr1 and for CD4 + T-cell activation by CD11b + DCs. Xcr1-expressing DCs in the SDLNs are composed of two different populations: migratory (CD103 hi ) DCs, which immigrate from the skin, and resident (CD8α hi ) DCs, which develop in the nodes. To characterize the dynamic interactions of these distinct DC populations with CD8 + T cells during their activation in vivo, we developed a photoconvertible reporter mouse strain, which permits us to distinctively visualize the migratory and resident subsets of Xcr1-expressing DCs. After leaving the skin, migratory DCs infiltrated to the deep T-cell zone of the SDLNs over 3 d, which corresponded to their half-life in the SDLNs. Intravital two-photon imaging showed that after soluble antigen immunization, the newly arriving migratory DCs more efficiently form sustained conjugates with antigen-specific CD8 + T cells than other Xcr1-expressing DCs in the SDLNs. These results offer in vivo evidence for differential contributions of migratory and resident cross-presenting DCs to CD8 + T-cell activation.dendritic cell | CD8 + T cell | cross-presentation | intravital two-photon imaging | photoconversion

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Tracking and quantification of dendritic cell migration and antigen trafficking between the skin and lymph nodes

Cited by 150 publications

References 50 publications

Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer’s patches

Sampling of Candida albicans and Candida tropicalis by Langerin-positive dendritic cells in mouse Peyer’s patches

Hallmarks of Tissue-Resident Lymphocytes

Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node

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