Tracing the lineage of tracing cell lineages

Stern, Claudio D.; Fraser, Scott E.

doi:10.1038/ncb0901-e216

Cited by 90 publications

(58 citation statements)

References 33 publications

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“…Disruption of the histone deacetylase HDA1 produces an M phase delay, consistent with its role in maintaining chromatin structure (28)(29)(30). Yeast lacking the ␤-glucan metabolic gene GAS1, known to be slow growing and to harbor cell wall defects (31), delay in M͞G 1 . Deletion of ZDS1, implicated in establishing cell polarity (32), delays cells in S phase near the approximate time of bud emergence.…”

Section: Large-scale Identification Of Cell Cycle Mutants By Using Exmentioning

confidence: 99%

“…The interactions between cells are fundamental to such biological processes as embryogenesis, differentiation and development, and oncogenesis, among others. Disentangling the dynamics of cell populations requires precise identification of cell types (1), ideally based on detailed measurements of molecular markers specific to each cell type (2). Identification of such markers is not trivial.…”

mentioning

confidence: 99%

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Expression deconvolution: A reinterpretation of DNA microarray data reveals dynamic changes in cell populations

Nakorchevskiy

Marcotte

2003

Proc. Natl. Acad. Sci. U.S.A.

126

138

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Section: Large-scale Identification Of Cell Cycle Mutants By Using Exmentioning

confidence: 99%

mentioning

confidence: 99%

Expression deconvolution: A reinterpretation of DNA microarray data reveals dynamic changes in cell populations

Nakorchevskiy

Marcotte

2003

Proc. Natl. Acad. Sci. U.S.A.

126

138

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“…Mapping cell fate in higher organisms, such as mice, containing a trillion or more cells, also has proven valuable but is more difficult (2,3) and has required manipulation of the embryo, in which cells are tagged via chimerism (4), dyes (5), radiation-induced cytogenetic abnormalities (6), or virally transferred (7), transgenic (8), or gene-targeted (9-11) markers. However, there are only a limited number of labels that can be applied to a single embryo.…”

mentioning

confidence: 99%

Phylogenetic fate mapping

Salipante¹,

Horwitz

2006

Proc. Natl. Acad. Sci. U.S.A.

110

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Cell fate maps describe how the sequence of cell division, migration, and apoptosis transform a zygote into an adult. Yet, it is only in Caenorhabditis elegans where microscopic observation of each cell division has allowed for construction of a complete fate map. More complex, and opaque, animals prove less yielding. DNA replication, however, generates somatic mutations. Consequently, multicellular organisms comprise mosaics where most cells acquire unique genomes that are potentially capable of delineating their ancestry. Here we take a phylogenetic approach to passively retrace embryonic relationships by deducing the order in which mutations have arisen during development. We show that polyguanine repeat DNA sequences are particularly useful genetic markers, because they frequently change length during mitosis. To demonstrate feasibility, we phylogenetically reconstruct the lineage of cultured mouse NIH 3T3 cells based on mutations affecting the length of polyguanine markers. We then employ whole genome amplification to genotype polyguanine markers in single cells taken from a mouse and use phylogenetics to infer the developmental relationships of the sampled tissues. The result is consistent with the present understanding of embryogenesis and demonstrates the large scale potential of this method for producing a complete mammalian cell fate at the resolution of a single cell.cell fate ͉ development ͉ genomic instability

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“…Tissue movements and deformations, while not as well studied, are equally important in understanding formation of the bilateral body plan of warm-blooded embryos (Fraser and Harland 2000;Keller et al, 2003;Czirok et al, 2004;Zamir et al, 2005). Embryologists have invented a number of techniques for selectively labeling live cells and/or tissues in order to study their movements (Graeper, 1929;Le Douarin, 1973;Muramatsu et al, 1996; for review: Stern and Fraser, 2001). The advent of genetically engineered fluorescent protein vectors is a powerful tool, particularly when coupled to modern microscopes and image detectors.…”

Section: Introductionmentioning

confidence: 99%

Electroporation and EGFP labeling of gastrulating quail embryos

Cui¹,

Lansford²,

Filla³

et al. 2006

Developmental Dynamics

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Labeling embryonic cells to trace their motion is a classical experimental approach with a host of techniques being used to mark live cells and tissues. Genetically engineered fluorescent protein vectors (DNA plasmids) are a recent technology well suited to time-resolved studies of cellular motion in live embryos. DNA plasmids encoding fluorescent proteins can be introduced into cells using several methods, including electroporation, a technique used widely for analysis of tissue culture and embryonic cells. Here we describe a technique designed to introduce DNA plasmids into early gastrulation stage quail embryos, ex ovo. The method is effective, and with practice enables an investigator to direct the vectors to relatively confined regions of gastrulating embryos. The required electroporation chamber can be fabricated from common laboratory materials. We anticipate that using this method of labeling cells in a warm-blooded embryo, during gastrulation, will be a fruitful means of studying subsequent embryogenesis. Developmental Dynamics 235:2802-2810, 2006.

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Tracing the lineage of tracing cell lineages

Cited by 90 publications

References 33 publications

Expression deconvolution: A reinterpretation of DNA microarray data reveals dynamic changes in cell populations

Expression deconvolution: A reinterpretation of DNA microarray data reveals dynamic changes in cell populations

Phylogenetic fate mapping

Electroporation and EGFP labeling of gastrulating quail embryos

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