Tracheal suture in rats with hypothyroidism: wound healing study

Zimmermann, Elise; Ribas-Filho, Jurandir Marcondes; Malafaia, Osvaldo; Ribas, Carmen Austrália Paredes Marcondes; Nassif, Paulo Afonso Nunes; Filho, Edmar Stieven; Przysiezny, Paulo Eduardo

doi:10.1590/s0102-86502009000400007

Cited by 16 publications

(13 citation statements)

References 29 publications

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“…With the model, reproduction of some clinical signs seen in humans and dogs are achieved. Such signs are skin alteration (alopecia) in rats [ 13 ] , alterations in cardiovascular systems, and reninangiotensin in sheep [ 14 ] , and reduction in collagen [ 15 ] . The models used in reproducing congenital hypothyroidism have advantages and disadvantages in their applications ( • ▶ Table 2 ).…”

Section: Description Of Models To Induce Hypothyroidism ▼ Surgical Modelmentioning

confidence: 99%

Experimental Models of Developmental Hypothyroidism

2011

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Hypothyroidism is a systemic disease resulting from either thyroid gland's anatomical and functional absence or lack of hypophyseal stimulation, both of which can lead to deficiency in thyroid hormone (TH) production. TH is essential for human and animal development, growth, and function of multiple organs. Children with deficient TH can develop alterations in central nervous system (CNS), striated muscle, bone tissue, liver, bone marrow, and cardiorespiratory system. Among the clinical outlook are signs like breathing difficulty, cardiac insufficiency, dysphagia, and repeated bronchial aspiration, constipation, muscle weakness, cognitive alterations, cochlear dysfunction, reduced height, defects in temperature regulation, anaemia, jaundice, susceptibility to infection, and others. Experimental and clinical studies have shown that TH is very essential for normal brain development. Other research work based on mice pointed out that a reduced level of TH in pregnant mother leads to congenital hypothyroidism in animal models and it is associated with mental retardation, deep neurologic deficiency that impacts on cognitive, learning, and memory functions. The principal experimental model studies that have focused on hypothyroidism are reviewed in this study. This is important on considering the fact that almost all animal species require thyroid hormones for their metabolism.

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Section: Description Of Models To Induce Hypothyroidism ▼ Surgical Modelmentioning

confidence: 99%

Experimental Models of Developmental Hypothyroidism

2011

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“…The influence of hypothyroidism on tissue repair has been few studied in the literature. In vivo study performed by Zimmermann et al (2009) 3 showed that hypothyroidism affects the healing process prolonging the inflammatory phase, decreasing the amount of collagen fibers and delaying its maturation and organization. Similar results were observed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Experimental studies have shown that the hypothyroid state has been associated to disruption of the body's metabolism, including healing process [1][2][3]. Thyroid hormones regulate body's metabolism and, among other actions, stimulate cellular differentiation and proliferation [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…Thyroid hormones regulate body's metabolism and, among other actions, stimulate cellular differentiation and proliferation [4][5][6]. When these hormones are below normal serum levels, they cause disturbances in the metabolic activation of the tissues, on secretion of fibroblasts, synthesis and deposition of collagen fibers [1][2][3], extending from the inflammatory phase to the proliferative phase, since are associated with proliferative process in wounds healing [7]. The literature has shown that LED light is able to increase production of collagen fiber by fibroblasts [8] and to promote positive biomodulating effects on wound healing [9].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of LED photobiomodulation on wound healing in hypothyroid and euthyroid rats

Paraguassú¹,

Xavier²,

Rodriguez³

et al. 2012

AIP Conference Proceedings

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Articles you may be interested inAssessment of the effects of laser or LED photobiomodulation on hypothyroid rats of cutaneous wound healing: A morphometric study.Evaluation of the effect of LED radiation in the repair of skin wounds on the dorsum of rats with iron deficiency anemia AIP Conf.Abstract. Thyroid hormone deficiency has been associated to disruption of the body's metabolism, including healing process. LED phototherapy has been shown to be effective in improving healing in many situations, but their usefulness in the improvement of hypothyroidism wound healing remains unknown. The aim of this study was to assess histologically the effect of LED (λ630 ± 20nm, 150mW, CW, φ=0.5 cm², 24J/cm² per session) on wounds healing in hypothyroid and euthyroid Wistar rats. Standard cutaneous wound (1cm²) was created on the dorsum of 24 animals divided into four groups of 6 animals each: G1: Euthyroid; G2: Euthyroid + LED; G3: Hypothyroid; G4: Hypothyroid + LED. Hypothyroidism was induced in rats with propylthiouracil (0.05g/100mL) administered orally for 4 weeks and maintained until the end of the experiment. The irradiation started immediately after surgery and was repeated every other day for 7 days. Animals were killed 8 days after surgery. The specimens were removed, routinely processed and stained with H&E and Sirius red. Statistical analysis was performed using the Fisher´s Test and significance level was 5%. The results of the present investigation showed delayed wounds healing process in hypothyroid rats characterized by incomplete reepithelialization and formation of disorganized collagen fibers (p<0.05) when compared to the euthyroid group, and that in the irradiated groups the reepithelialization was statistically higher when compared with non-irradiated ones (p<0.05). There was improved collagen fibers deposition on irradiated animals (euthyroid and hypothyroid) when compared to non-irradiated ones, but no statistically significant difference (p>0.05). It is concluded that LED light has a positive biomodulative effect on the wound healing process, even when hipothyroidism was present.

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“…The reduction of the inflammatory cells in the PTP NF group could be due to the earlier progression of healing to the next phase, resulting in a faster recovery compared with the control group (which shows a slow progress). In addition, the role of thyroid hormone in protecting, preventing, and reducing inflammatory responses are reported earlier (Contreras-Jurado et al, 2011;Perrotta et al, 2014;Rittenhouse & Redei, 1997;Zimmermann et al, 2009). Hence, the number of inflammatory cells could be reduced in the group treated with PTP NF due to the property of T 3 to protect from excessive inflammation and also due to the enhanced healing response observed.…”

Section: Animal Experimentsmentioning

confidence: 94%

Strategic design of peptide‐decorated aligned nanofibers impregnated with triiodothyronine for neural regeneration

Satish

Korrapati

2019

J Tissue Eng Regen Med

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Nerve injuries are often debilitating as its regeneration occurs in a slow and laborious manner. Remediation of nerve injury is a colossal task as functional restoration in larger gaps seldom occurs due to the complex nerve regeneration mechanism. A nanofiber‐based graft material has been fabricated to provide topographical and biochemical cues to encourage neural differentiation. Laminin plays a crucial role in supporting peripheral nerve regeneration and hence aligned polyvinyl cinnamate nanofibers surface‐conjugated with laminin‐derived cell‐adhesion peptides have been fabricated to improve selective neural adhesion and regeneration. Further, triiodothyronine has been encapsulated within the nanofibers enabling its sustained release so as to bolster regeneration and reinstate the lost functionality to the damaged nerve. The fabricated nanofibers were characterized for its physicochemical, morphological, and topographical properties. Nanofibers were biocompatible, improved cell adhesion rate, and illustrated favourable interaction with cells. Gene expression (showed 9.5 and 4.1 fold increase in β‐tubulin and MAP 2 expression, respectively) and protein expression (immunofluorescence, flow cytometry, and western blot) studies confirmed the positive influence of the scaffold over cell differentiation. The studies were extrapolated to adult zebrafish model with a surgical incision in posterior lateral line. The biocomposite treated group showed earlier functional restoration of the nerve compared with control groups detected by touch‐evoked response. Thus, the combination of aligned nanofibers providing topographical cue, along with the peptides and triiodothyronine serving as biochemical cues, has a robust potential to restore functionality to the injured nerve, thereby opening avenues for fabrication of regenerative nerve grafts.

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Tracheal suture in rats with hypothyroidism: wound healing study

Cited by 16 publications

References 29 publications

Experimental Models of Developmental Hypothyroidism

Experimental Models of Developmental Hypothyroidism

Evaluation of LED photobiomodulation on wound healing in hypothyroid and euthyroid rats

Strategic design of peptide‐decorated aligned nanofibers impregnated with triiodothyronine for neural regeneration

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