Toxicology of 3-epi-deoxynivalenol, a deoxynivalenol-transformation product by Devosia mutans 17-2-E-8

He, Jianlin; Bondy, Genevieve S.; Zhou, Ting; Caldwell, Douglas R.; Boland, G. J.; Scott, Peter

doi:10.1016/j.fct.2015.09.003

Cited by 90 publications

(78 citation statements)

References 38 publications

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“…Specific cell lines may be more or less suitable for testing certain toxins. For example, He et al (2015) [127] used heterogeneous human epithelial colorectal adenocarcinoma (Caco-2) cells and mouse embryonic fibroblast (3T3) cells to assess cell viability and DNA synthesis, respectively, and thereby to illustrate the diminished toxicity of 3- epi -DON. Recently, mouse models have been gaining more attention and becoming feasible routes for bio-potency testing.…”

Section: Strategies and Methodologiesmentioning

confidence: 99%

“…Recently, mouse models have been gaining more attention and becoming feasible routes for bio-potency testing. He et al (2015) [127] followed the above cell culture assays by exposing B6C3F1 mice to DON and 3- epi -DON treatments for 14 consecutive days, demonstrating that toxin-induced lesions were only observed in the adrenal glands, thymus, stomach, spleen and colon of the DON-group, but not 3- epi -DON. Some alternative approaches for using large animals in toxicity testing have been devised recently.…”

Section: Strategies and Methodologiesmentioning

confidence: 99%

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Strategies and Methodologies for Developing Microbial Detoxification Systems to Mitigate Mycotoxins

Zhu

Hassan

Lepp

et al. 2017

Toxins

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Mycotoxins, the secondary metabolites of mycotoxigenic fungi, have been found in almost all agricultural commodities worldwide, causing enormous economic losses in livestock production and severe human health problems. Compared to traditional physical adsorption and chemical reactions, interest in biological detoxification methods that are environmentally sound, safe and highly efficient has seen a significant increase in recent years. However, researchers in this field have been facing tremendous unexpected challenges and are eager to find solutions. This review summarizes and assesses the research strategies and methodologies in each phase of the development of microbiological solutions for mycotoxin mitigation. These include screening of functional microbial consortia from natural samples, isolation and identification of single colonies with biotransformation activity, investigation of the physiological characteristics of isolated strains, identification and assessment of the toxicities of biotransformation products, purification of functional enzymes and the application of mycotoxin decontamination to feed/food production. A full understanding and appropriate application of this tool box should be helpful towards the development of novel microbiological solutions on mycotoxin detoxification.

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Section: Strategies and Methodologiesmentioning

confidence: 99%

Section: Strategies and Methodologiesmentioning

confidence: 99%

Strategies and Methodologies for Developing Microbial Detoxification Systems to Mitigate Mycotoxins

Zhu

Hassan

Lepp

et al. 2017

Toxins

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“…The product 3-keto-DON is approximately 90% less toxic than DON, and represents a suitable detoxified product [21]. Shima et al [21] discovered a single organism in aerobic conditions from an environmental sample that converted DON into 3-keto-DON, and He et al [22,23] isolated an aerobic organism, from the genus Devosia , converting DON to 3-epimer-DON (3-epi-DON). Ikunaga et al [24] identified a bacterium from the genus Nocardioides that converts DON to 3-epi-DON.…”

Section: Introductionmentioning

confidence: 99%

“…Based on previous work [21,22,23,24,25], we hypothesized that mixed cultures of microorganisms isolated from natural soil environments incubated with a mineral salt media using 100 μg/mL DON as the sole carbon source will detoxify DON. The specific objectives of this research were as follows: (1) identify microbes isolated from plant and soil samples taken in Blacksburg, VA, that modify DON; (2) characterize DON metabolites using thin layer chromatography (TLC), gas chromatography mass spectrometry (GC/MS), and nuclear magnetic resonance (NMR); (3) identify bacterial components of mixed cultures with DON modification activity; and (4) determine if these microorganisms can modify DON in naturally contaminated wheat samples.…”

Section: Introductionmentioning

confidence: 99%

Modification of the Mycotoxin Deoxynivalenol Using Microorganisms Isolated from Environmental Samples

Wilson

McMaster

Gantulga

et al. 2017

Toxins

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The trichothecene mycotoxin deoxynivalenol (DON) is a common contaminant of wheat, barley, and maize. New strategies are needed to reduce or eliminate DON in feed and food products. Microorganisms from plant and soil samples collected in Blacksburg, VA, USA, were screened by incubation in a mineral salt media containing 100 μg/mL DON and analysis by gas chromatography mass spectrometry (GC/MS). Two mixed cultures derived from soil samples consistently decreased DON levels in assays using DON as the sole carbon source. Nuclear magnetic resonance (NMR) analysis indicated that 3-keto-4-deoxynivalenol was the major by-product of DON. Via 16S rRNA sequencing, these mixed cultures, including mostly members of the genera Acinetobacter, Leadbetterella, and Gemmata, were revealed. Incubation of one of these mixed cultures with wheat samples naturally contaminated with 7.1 μg/mL DON indicated nearly complete conversion of DON to the less toxic 3-epimer-DON (3-epi-DON). Our work extends previous studies that have demonstrated the potential for bioprospecting for microorganisms from the environment to remediate or modify mycotoxins for commercial applications, such as the reduction of mycotoxins in fuel ethanol co-products.

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“…Earlier reported mechanisms of DON microbial detoxification have focused on de‐epoxidation, transformation of the C3 carbon (or attached groups), or the complete mineralization of DON (Karlovsky, 2011; Zhu et al ., 2016). Reported modifications of C3 include oxidation and epimerization of the attached hydroxyl group, which was demonstrated to reduce toxicity (He et al ., 2015a,b). Devosia mutans 17‐2‐E‐8 ( Devosia spp.…”

Section: Introductionmentioning

confidence: 99%

The enzymatic detoxification of the mycotoxin deoxynivalenol: identification of DepA from the DON epimerization pathway

Carere

Hassan

Lepp

et al. 2017

Microbial Biotechnology

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SummaryThe biological detoxification of mycotoxins, including deoxynivalenol (DON), represents a very promising approach to address the challenging problem of cereal grain contamination. The recent discovery of Devosia mutans 17‐2‐E‐8 (Devosia spp. 17‐2‐E‐8), a bacterial isolate capable of transforming DON to the non‐toxic stereoisomer 3‐epi‐deoxynivalenol, along with earlier reports of bacterial species capable of oxidizing DON to 3‐keto‐DON, has generated interest in the possible mechanism and enzyme(s) involved. An understanding of these details could pave the way for novel strategies to manage this widely present toxin. It was previously shown that DON epimerization proceeds through a two‐step biocatalysis. Significantly, this report describes the identification of the first enzymatic step in this pathway. The enzyme, a dehydrogenase responsible for the selective oxidation of DON at the C3 position, was shown to readily convert DON to 3‐keto‐DON, a less toxic intermediate in the DON epimerization pathway. Furthermore, this study provides insights into the PQQ dependence of the enzyme. This enzyme may be part of a feasible strategy for DON mitigation within the near future.

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Toxicology of 3-epi-deoxynivalenol, a deoxynivalenol-transformation product by Devosia mutans 17-2-E-8

Cited by 90 publications

References 38 publications

Strategies and Methodologies for Developing Microbial Detoxification Systems to Mitigate Mycotoxins

Strategies and Methodologies for Developing Microbial Detoxification Systems to Mitigate Mycotoxins

Modification of the Mycotoxin Deoxynivalenol Using Microorganisms Isolated from Environmental Samples

The enzymatic detoxification of the mycotoxin deoxynivalenol: identification of DepA from the DON epimerization pathway

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