Toxicological impact of organic ultrafine particles (UFPs) in human bronchial epithelial BEAS-2B cells at air-liquid interface

Facio, A.T. Juárez; Yon, Jérôme; Corbière, Cécile; Rogez-Florent, Tiphaine; Castilla, Clément; Lavanant, Hélène; Mignot, Mélanie; Devouge-Boyer, Christine; Logie, C.; Chevalier, Laurence; Vaugeois, Jean-Marie; Monteil, Christelle

doi:10.1016/j.tiv.2021.105258

Cited by 14 publications

(12 citation statements)

References 59 publications

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“…3 Ultrafine particles (UFP, aerodynamic diameter ≤100 nm) are of particular interest due to their high particle number concentrations, as well as high capability to adsorb toxic compounds to their surface and the ability to transport these compounds deep into the respiratory system. 1,4 Electrospray ionization (ESI) is a frequently applied ionization technique for the analysis of liquid aerosol extracts, due to its softness and sensitive ionization of polar compounds (e.g., oxygen-containing functionalities), which are frequently found in aerosol samples. 5,6 Nonpolar compounds like PAHs are usually not observed under standard ESI conditions, due to their poor ionization efficiency and ionization suppression from more readily ionized polar molecules.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Anthropogenic emission of particulate matter from industrial or urban sources like engines or biomass burning, is responsible for global adverse health effects . Ultrafine particles (UFP, aerodynamic diameter ≤100 nm) are of particular interest due to their high particle number concentrations, as well as high capability to adsorb toxic compounds to their surface and the ability to transport these compounds deep into the respiratory system. , …”

Section: Introductionmentioning

confidence: 99%

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Detection of Polycyclic Aromatic Hydrocarbons in High Organic Carbon Ultrafine Particle Extracts by Electrospray Ionization Ultrahigh-Resolution Mass Spectrometry

Schneider

Giocastro

Rüger

et al. 2022

J. Am. Soc. Mass Spectrom.

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The detection of polycyclic aromatic hydrocarbons (PAHs) by electrospray ionization (ESI) without additional reagents or targeted setup changes to the ionization source was observed in ultrafine particle (UFP) extracts, with high organic carbon (OC) concentrations, generated by a combustion aerosol standard (CAST) soot generator. Particulate matter (PM) was collected on filters, extracted with methanol, and analyzed by ESI Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Next to oxygen-containing species, pure hydrocarbons were found to be one of the most abundant compound classes, detected as [M + Na]+ or [M + H]+ in ESI+ and mostly as [M – H]− in ESI–. The assigned hydrocarbon elemental compositions are identified as PAHs due to their high aromaticity index (AI > 0.67) and were additionally confirmed by MS/MS experiments as well as laser desorption ionization (LDI). Thus, despite the relatively low polarity, PAHs have to be considered in the molecular attribution of these model aerosols and/or fresh emissions with low salt content investigated by ESI.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Polycyclic Aromatic Hydrocarbons in High Organic Carbon Ultrafine Particle Extracts by Electrospray Ionization Ultrahigh-Resolution Mass Spectrometry

Schneider

Giocastro

Rüger

et al. 2022

J. Am. Soc. Mass Spectrom.

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“…The methods to analyze particle and gaseous phases are the same as those described in previous works [ 29 ]. Briefly, samples were collected in Tedlar bags for gaseous emissions and then analyzed by gas chromatography with a thermal conductivity detector (GC/TCD) (SCION Instruments UK Ltd., Scotland, UK) and gas chromatography coupled to a mass spectrometer (GC/MS) (PerkinElmer, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The MTT assay is a common method to assess cell viability. The protocol was previously described [ 29 ]. Briefly, 3 h and 24 h after exposure, cells were incubated with MTT solution for 3 h, and then formazan crystals were dissolved with SDS solution (10%).…”

Section: Methodsmentioning

confidence: 99%

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Ultrafine Particles Issued from Gasoline-Fuels and Biofuel Surrogates Combustion: A Comparative Study of the Physicochemical and In Vitro Toxicological Effects

Juárez-Facio

Rogez-Florent

Meausoone

et al. 2022

Toxics

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Gasoline emissions contain high levels of pollutants, including particulate matter (PM), which are associated with several health outcomes. Moreover, due to the depletion of fossil fuels, biofuels represent an attractive alternative, particularly second-generation biofuels (B2G) derived from lignocellulosic biomass. Unfortunately, compared to the abundant literature on diesel and gasoline emissions, relatively few studies are devoted to alternative fuels and their health effects. This study aimed to compare the adverse effects of gasoline and B2G emissions on human bronchial epithelial cells. We characterized the emissions generated by propane combustion (CAST1), gasoline Surrogate, and B2G consisting of Surrogate blended with anisole (10%) (S+10A) or ethanol (10%) (S+10E). To study the cellular effects, BEAS-2B cells were cultured at air-liquid interface for seven days and exposed to different emissions. Cell viability, oxidative stress, inflammation, and xenobiotic metabolism were measured. mRNA expression analysis was significantly modified by the Surrogate S+10A and S+10E emissions, especially CYP1A1 and CYP1B1. Inflammation markers, IL-6 and IL-8, were mainly downregulated doubtless due to the PAHs content on PM. Overall, these results demonstrated that ultrafine particles generated from biofuels Surrogates had a toxic effect at least similar to that observed with a gasoline substitute (Surrogate), involving probably different toxicity pathways.

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Biological impact of sequential exposures to allergens and ultrafine particle‐rich combustion aerosol on human bronchial epithelial BEAS‐2B cells at the air liquid interface

Zimmermann

Candeias

Gawlitta

et al. 2023

J of Applied Toxicology

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The prevalence of allergic diseases is constantly increasing since few decades.Anthropogenic ultrafine particles (UFPs) and allergenic aerosols is highly involved in this increase; however, the underlying cellular mechanisms are not yet understood.Studies observing these effects focused mainly on singular in vivo or in vitro exposures of single particle sources, while there is only limited evidence on their subsequent or combined effects. Our study aimed at evaluating the effect of subsequent exposures to allergy-related anthropogenic and biogenic aerosols on cellular mechanism exposed at air-liquid interface (ALI) conditions. Bronchial epithelial BEAS-2B cells were exposed to UFP-rich combustion aerosols for 2 h with or without allergen pre-exposure to birch pollen extract (BPE) or house dust mite extract (HDME). The physicochemical properties of the generated particles were characterized by stateof-the-art analytical instrumentation. We evaluated the cellular response in terms of cytotoxicity, oxidative stress, genotoxicity, and in-depth gene expression profiling.We observed that single exposures with UFP, BPE, and HDME cause genotoxicity.Exposure to UFP induced pro-inflammatory canonical pathways, shifting to a more xenobiotic-related response with longer preincubation time. With additional allergen exposure, the modulation of pro-inflammatory and xenobiotic signaling was more pronounced and appeared faster. Moreover, aryl hydrocarbon receptor (AhR) signaling activation showed to be an important feature of UFP toxicity, which was especially pronounced upon pre-exposure. In summary, we were able to demonstrate the importance of subsequent exposure studies to understand realistic exposure situations and to identify possible adjuvant allergic effects and the underlying molecular mechanisms.

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Toxicological impact of organic ultrafine particles (UFPs) in human bronchial epithelial BEAS-2B cells at air-liquid interface

Cited by 14 publications

References 59 publications

Detection of Polycyclic Aromatic Hydrocarbons in High Organic Carbon Ultrafine Particle Extracts by Electrospray Ionization Ultrahigh-Resolution Mass Spectrometry

Detection of Polycyclic Aromatic Hydrocarbons in High Organic Carbon Ultrafine Particle Extracts by Electrospray Ionization Ultrahigh-Resolution Mass Spectrometry

Ultrafine Particles Issued from Gasoline-Fuels and Biofuel Surrogates Combustion: A Comparative Study of the Physicochemical and In Vitro Toxicological Effects

Biological impact of sequential exposures to allergens and ultrafine particle‐rich combustion aerosol on human bronchial epithelial BEAS‐2B cells at the air liquid interface

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