Toxicological evaluation of feed contaminated with mycotoxins using a luminescent microorganism

Orobchenko, O. L.; Курбацька, Олена Володимирівна; Paliy, Anatoliy; Palii, A.P.

doi:10.46419/vs.54.2.7

Cited by 3 publications

(2 citation statements)

References 21 publications

(26 reference statements)

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“…Luminous cells are actively used to assess the toxicity of agricultural products contaminated with mycotoxins [27,28]. However, it was shown for the first time in this work that immobilized photobacteria can be used to determine the level of toxicity of media with a higher number (in our case, ten) of different mycotoxins (Figure 2).…”

Section: Discussionmentioning

confidence: 86%

Luminescent Bacteria as Bioindicators in Screening and Selection of Enzymes Detoxifying Various Mycotoxins

Efremenko,

Lyagin,

Stepanov

et al. 2024

Sensors

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Interest in enzymes capable of neutralizing various mycotoxins is quite high. The methods used for the screening and selection of enzymes that catalyze the detoxification of mycotoxins should be sensitive and fast. However toxic compounds can be generated under the action of such enzymes. Thus, the assessment of the overall reduction in the toxic properties of reaction media towards bioluminescent bacteria seems to be the most reasonable control method allowing a quick search for the effective enzymatic biocatalysts. The influence of a wide range of mycotoxins and glucanases, which hydrolyze toxins with different chemical structures, on the analytical characteristics of luminescent photobacteria as a biosensing element has been studied. Different glucanases (β-glucosidase and endoglucanase) were initially selected for reactions with 10 mycotoxins based on the results of molecular docking which was performed in silico with 20 mycotoxins. Finally, the biorecognizing luminescent cells were used to estimate the residual toxicity of reaction media with mycotoxins after their interaction with enzymes. The notable non-catalytic decrease in toxicity of media containing deoxynivalenol was revealed with luminous cells for both types of tested glucanases, whereas β-glucosidase provided a significant catalytic detoxification of media with aflatoxin B2 and zearalenone at pH 6.0.

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Section: Discussionmentioning

confidence: 86%

Luminescent Bacteria as Bioindicators in Screening and Selection of Enzymes Detoxifying Various Mycotoxins

Efremenko,

Lyagin,

Stepanov

et al. 2024

Sensors

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“…За дії антиоксидантів (вітаміну С, Nацетилцистеїну і астаксантину) in vitro на модель клітин гепатоцелюлярної карциноми печінки людини (HepG2) всі три речовини були ефективними щодо зменшення накопичення жиру в гепатоцитах: зменшувалися ліпідні краплі, концентрація тригліцеридів, знижувалося утворення АФО і наявність клітин апоптозом та експресією генів ендоплазматичного ретикулуму (Yang et al, 2018). В інших дослідженнях (Orobchenko, 2011) -надходження до організму курей-несучок добавок інших антиоксидантів альфа-токоферолу ацетату та натрію селеніту в дозах 1000,0 та 1,0 г/т з кормом протягом 28 діб викликало у птиці гіпохолестеринемію та гіполіпідемію та узгоджується з отриманими нами результатами: зниженням показників ліпідного обміну в організмі щурів протягом тривалого введення NP GdVO 4 :Eu 3+ , що підтверджує ствердження щодо потенційної антиоксидантної дії наночастинок металів.…”

Section: таблицяunclassified

The state of metabolic parameters of the blood in white rats under conditions of long-term oral administration of gadolinium orthovanadate nanoparticles under food stress

et al. 2023

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The prohibition of using antibiotics with growth-stimulating properties in the European Union led to the search for new, safer, more effective, and cheaper feed additives. One of these substances was rare earth elements (REE, atomic numbers 57–71) due to their low toxicity and protective and antioxidant properties. At the same time, higher efficiency of organic forms of REE was noted. Thanks to this, it is possible to assume their even more pronounced effect in nano-sized form as feed additives and the need to conduct research in this direction. Therefore, this work aims to study the metabolic parameters of the blood in rats under long-term oral administration of nanoparticles of one of the representatives of rare earth elements – gadolinium orthovanadate (NP GdVO4:Eu3+) under food stress. Experimental samples of gadolinium orthovanadate nanoparticles activated by Europium (spindle-shaped geometry; size 8×25 nm; initial concentration 1.0 g/dm3) were used in work. Experimental studies on rats were carried out based on the vivarium of the NSC “IEKVM”. The object of research was 140 mature male Wistar rats with an initial weight of 180–200 g. Four groups of animals, 35 rats each, were formed according to the principle of analogs. During the experiment, animals of the control group received drinking water without additives; rats of the І experimental group were given a solution of NP GdVO4:Eu3+ at a dose of 0.2 mg/dm3 (≈ 0.03 mg/kg of body weight); II research group – at a dose of 1.0 mg/dm3 (≈ 0.15 mg/kg of body weight) and rats of III research group – at a dose of 2.0 mg/dm3 (≈ 0.30 mg/kg of body weight). Drinking was carried out for 56 days, then it was completed, and the rats were observed for another 14 days. A nutritionally unbalanced diet was used as a stress factor. Taking into account the results of biochemical studies, the adaptogenic effect of NP GdVO4:Eu3+ in the range of doses of 0.2–1.0 mg/dm3 of drinking water (≈ 0.03–0.15 mg/kg of body weight) on the body of white rats was established under conditions of food stress with optimal duration of action – 28–42 days. Under the conditions of administration at a dose of 2.0 mg/dm3 of drinking water (≈ 0.30 mg/kg of body weight), a hepatic(cyto-)toxic effect of nanoparticles was detected, which was accompanied by an irreversible decrease in the structural indicators of lipid metabolism, the consumption of antioxidant resources and the induction of intensity processes of lipid peroxidation against the background of alanine aminotransferase hyperenzymemia.

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Formation of the biological value of beef protein depending on the age and breed of bulls

Paliy,

Michalchenko,

Korkh

et al. 2024

Potr. S. J. F. Sci.

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The article substantiates the expediency of assessing the content of essential amino acids in the samples obtained during the controlled slaughter of bulls for the protein of chicken eggs as an effective means of improving the quality of the management processes for producing biologically complete products. To ascertain the biological value of beef, samples of the longest back muscle were obtained from bulls of six domestic dairy breeds (Black-and-White, Red Steppe, and Angler dairy breeds and Simmental, Lebedyn, and Gray Ukrainian dairy breeds of combined productivity) at the ages of 3, 6, 9, 12, 15, 18, and 21 months. The experiments were conducted using the ion-exchange liquid column chromatography method on an automatic amino acid analyser (T-339 M) manufactured by Microtechna (Czech Republic). It has been demonstrated that the biological value of meat from bulls of the studied breeds is limited during ontogeny, primarily due to age-related factors. As bulls grow older and gain weight, their meat proteins exhibit increased biological value, approaching the reference index of chicken egg proteins. The first peak in the average values of the amino acid index in beef of bulls of 12 months of age (0.89%) was followed by a consistent decrease to 15 months (0.68%) and a repeated increase in values in animals of 21 months of age (0.83%). This is mainly due to the rise in the scores for methionine by 0.16%, isoleucine by 0.16%, histidine by 0.42%, arginine by 0.18%, and threonine by 0.20%. The increase in the biological value of the remaining amino acid scores in the age trend of changes did not exceed 0.15%. The identified patterns indicate the presence of additional reserves in the near-term scenario, which can be utilised to ensure the production of high-grade beef while optimising the age parameters of slaughtering bulls of different productivity directions.

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Toxicological evaluation of feed contaminated with mycotoxins using a luminescent microorganism

Cited by 3 publications

References 21 publications

Luminescent Bacteria as Bioindicators in Screening and Selection of Enzymes Detoxifying Various Mycotoxins

Luminescent Bacteria as Bioindicators in Screening and Selection of Enzymes Detoxifying Various Mycotoxins

The state of metabolic parameters of the blood in white rats under conditions of long-term oral administration of gadolinium orthovanadate nanoparticles under food stress

Formation of the biological value of beef protein depending on the age and breed of bulls

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