Toxicological effects of trace amounts of pyriproxyfen on the midgut of non-target insect silkworm

Xu, Kai; Lan, Huangli; He, Chunhui; Wei, Yuting; Lu, Qingyu; Cai, Kunpei; Yu, Dongliang; Yin, Xingcan; Li, Yizhe; Lv, Jiachen

doi:10.1016/j.pestbp.2022.105266

Cited by 14 publications

(9 citation statements)

References 51 publications

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“…After oral delivery, the gut is the first tissue in contact with the insecticide, and is divided into three zones (foregut, midgut, and hindgut). The use of insecticides causes changes in several parts of the insect, including the midgut [36]. This organ section is a region of digestion and absorption of nutrients modulated by the gut microbiota, housing one of the main defense mechanisms against pathogenic microorganisms [37].…”

Section: Discussionmentioning

confidence: 99%

Deleterious Effects of Cymbopogon nardus (L.) Essential Oil on Life Cycle and Midgut of the Natural Predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Caballero-Gallardo

Scudeler

Santos

et al. 2023

Insects

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Cymbopogon nardus (citronella) essential oil (EO) has been widely used in the cosmetic and food industry due to its repellent and fumigant properties. The aim of this study was to evaluate its effects on the life cycle and midgut morphology of the natural predator Ceraeochrysa claveri. Larvae were fed on sugarcane borer eggs (Diatraea saccharalis) pretreated with citronella EO solutions (1–100 µg/mL in methanol, 5 s) or solvent and air-dried at room temperature for 30 min. Larval and pupal stage duration, the percentage of emergence of the insect, and malformed insects were recorded. One day after adults emerged from their cocoons, adult insects were used to obtain their midgut and analyzed using light microscopy. The chemical composition of C. nardus EO revealed that citronellal (25.3%), citronellol (17.9%), geraniol (11.6%), elemol (6.5%), δ-cadinone (3.6%), and germacrene D (3.4%) were the predominant compounds. Exposure to the EO produced a significant change in development duration for third instar and prepupa of the insect. The observed alterations in the lifecycle included prepupae with no cocoon formation, dead pupa inside the cocoon, and malformed adults. Several injuries in the midgut epithelium of exposed adults were registered, such as detachment of columnar cells leaving only swollen regenerative cells fixed on the basement membrane, and the formation of epithelial folds. In summary, these data suggest that C. nardus oil has adverse effects on the life cycle and midgut morphology of a beneficial predator.

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Section: Discussionmentioning

confidence: 99%

Deleterious Effects of Cymbopogon nardus (L.) Essential Oil on Life Cycle and Midgut of the Natural Predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Caballero-Gallardo

Scudeler

Santos

et al. 2023

Insects

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“…Moreover, the lipase activity in the intestinal fluid in the 3L 0 h and 5L 0 h groups was higher than that in the AD group (Figure S3B). We therefore speculate that dietary transition from ADs to MLs improves the activity of silkworm intestinal cells, thereby facilitating nutrient digestion and absorption [40].…”

Section: Dietary Transition Reshapes the Gut Microbiota Of Silkwormsmentioning

confidence: 89%

Effects of Habitual Dietary Change on the Gut Microbiota and Health of Silkworms

Wang,

Ding,

Yang

et al. 2024

IJMS

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Diet plays a crucial role in shaping the gut microbiota and overall health of animals. Traditionally, silkworms are fed fresh mulberry leaves, and artificial diets do not support good health. The aim of this study was to explore the relationship between the dietary transition from artificial diets to mulberry leaves and the effects on the gut microbiota and physiological changes in silkworms as a model organism. With the transition from artificial diets to mulberry leaves, the diversity of the silkworm gut microbiota increased, and the proportion of Enterococcus and Weissella, the dominant gut bacterial species in silkworms reared on artificial diets, decreased, whereas the abundance of Achromobacter and Rhodococcus increased. Dietary transition at different times, including the third or fifth instar larval stages, resulted in significant differences in the growth and development, immune resistance, and silk production capacity of silkworms. These changes might have been associated with the rapid adaptation of the intestinal microbiota of silkworms to dietary transition. This study preliminarily established a dietary transition–gut microbial model in silkworms based on the conversion from artificial diets to mulberry leaves, thus providing an important reference for future studies on the mechanisms through which habitual dietary changes affect host physiology through the gut microbiome.

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“…In fact, pesticide poisoning has been noted as one of the major impediments to the sericulture industry, accounting for more than 30% of the annual loss in China 2 . The toxicity of low‐dose pesticides to silkworms has aroused concerns of researchers, and sublethal effects of some pesticides on silkworms have been partially investigated 3–5 . Previous studies have shown that long‐term exposure to trace amounts of pesticides, such as acetamiprid, phoxim and chlorantraniliprole, can lead to growth retardation, weight loss, disruption of molting and pupation, and reduced spinning ability in silkworms 3,6–8 .…”

Section: Introductionmentioning

confidence: 99%

“…2 The toxicity of low-dose pesticides to silkworms has aroused concerns of researchers, and sublethal effects of some pesticides on silkworms have been partially investigated. [3][4][5] Previous studies have shown that long-term exposure to trace amounts of pesticides, such as acetamiprid, phoxim and chlorantraniliprole, can lead to growth retardation, weight loss, disruption of molting and pupation, and reduced spinning ability in silkworms. 3,[6][7][8] On the other hand, few studies have focused on remedies for pesticide-poisoned silkworms.…”

mentioning

confidence: 99%

Characterization of the sublethal toxicity and transcriptome‐wide biological changes induced by λ‐cyhalothrin in Bombyx mori

Ren

Wang

et al. 2023

Environmental Toxicology

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λ‐Cyhalothrin (λ‐cyh) is widely used in agricultural production and has been reported to cause damages to numerous nontarget insects. As an important economic and model insect of Lepidoptera, Bombyx mori was extremely sensitive to λ‐cyh, and pesticide drift often leads to silkworm poisoning. However, little is known about the persistence of sublethal effects or the potential recovery from short‐term exposure to sublethal doses of pesticides. In this study, we estimated the sublethal effects caused by short‐term exposure (24 h) of λ‐cyh LC1, LC10, LC25, and LC50, respectively, and investigated the persistent negative effects on the growth, survival, and pupal metamorphosis of silkworm larvae. Silkworm growth was mostly retarded after λ‐cyh exposure, with dose‐dependent recovery observed at delayed time points. Relative to the control, the treatment groups showed significantly higher larval mortalities and abnormal pupa rates. Additionally, transcriptome sequencing was conducted to investigate the effects of λ‐cyh LC10 on the normal physiological functions in the midgut of B. mori. A total of 2697 differentially expressed genes were identified, and 57.1% of DEGs were down‐regulated. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis further revealed that energy and nutrient metabolisms were negatively affected. Moreover, we demonstrated that sublethal λ‐cyh inhibited the oxidative phosphorylation pathway by reducing the expression of mitochondrial electron transport chain complex genes and consequently the synthesis of ATP. This study has provided useful transcriptome‐wide expression resources to facilitate the overall knowledge of the molecular basis of sublethal toxicity caused by λ‐cyh in the midgut of B. mori.

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Toxicological effects of trace amounts of pyriproxyfen on the midgut of non-target insect silkworm

Cited by 14 publications

References 51 publications

Deleterious Effects of Cymbopogon nardus (L.) Essential Oil on Life Cycle and Midgut of the Natural Predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Deleterious Effects of Cymbopogon nardus (L.) Essential Oil on Life Cycle and Midgut of the Natural Predator Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

Effects of Habitual Dietary Change on the Gut Microbiota and Health of Silkworms

Characterization of the sublethal toxicity and transcriptome‐wide biological changes induced by λ‐cyhalothrin in Bombyx mori

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