Toxicological analyses: analytical method validation for prevention or diagnosis

Abrão, Lailah Cristina de Carvalho; Silveira, Alberto Thalison; Faria, Henrique Dipe de; Machado, Simone Caetani; Mendes, Tássia Venga; Placido, Rodrigo Vicentino; Marciano, Luiz Paulo de Aguiar; Martins, Ignez Salas

doi:10.1080/15376516.2020.1839612

Cited by 5 publications

(2 citation statements)

References 99 publications

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“…Thus, linear regression using the method of least squares was applied to fit the standard curve (Figure 2A) and showed an excellent correlation (r > 0.99) in the range of 5 -30 μg/mL (Table 2), as recommended by guidelines such as the ICH (ICH, 2005). Since the regression coefficient is closer to 1.0, the dispersion of the set of experimental points is considered smaller, with low uncertainty of the estimated regression coefficients (Abrão et al, 2020;ANVISA, 2017;ICH, 2005). The linear regression equation was y = 0.02711x + 0.2346, where y is the absorbance and x is the nominal concentration of SA (μg/mL).…”

Section: Linearitymentioning

confidence: 99%

Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil

Rocha

Viana

Silveira

et al. 2022

RSD

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The demand for analytical methods for drug quantification is an obstacle in the research and development of new products, due to the need to use simpler techniques for the identification and quantification of the actives. Although it is a very sensitive and selective technique for these determinations, high performance liquid chromatography (HPLC) requires expensive instrumentation and well-trained analysts, while spectrophotometry is fast, low-cost and hardly requires sample pre-treatment. Thus, this study aimed to develop a quantification method by UV-visible spectrophotometry for salicylic acid (SA) encapsulated in nanostructured lipid carriers (NLC) containing copaiba oil. The method was validated according to national and international criteria (ANVISA, ICH and FDA). The diluent was a mixture of absolute ethanol and phosphoric acid (99:1) and detection was performed at 310 nm. The interference of the matrix in relation to NLC-SA was minimal (0.37%), demonstrating the selectivity. Linearity was obtained in the range of 5.0 30.0 μg/mL (r = 0.99948) and the method proved to be accurate, with high recovery of SA in the presence of the other components (over 96% recovery), with precision at two levels: intra- and inter-days (DPR < 5%). Detection and quantification limits were 0.51 e 1.70 μg/mL, respectively. Finally, according to the Youden test criteria, the method was robust in the face of the proposed variations, obtaining effect values lower than the critical value (0.07). Therefore, the developed method is accessible, effective and valid for the quantification of SA in complex matrix lipid nanoparticles.

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Section: Linearitymentioning

confidence: 99%

Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil

Rocha

Viana

Silveira

et al. 2022

RSD

View full text Add to dashboard Cite

show abstract

“…The figure also indicates the use of high‐end instruments, such as HPLC, GC‐MS, and LC‐MS, to determine specific CEC concentrations. Analytical validation in the toxicological analysis is important to establish the precise exposure value for risk assessment [8]. Thus, developing an instrumental method in CEC analysis is essential to determine an exact and accurate risk assessment level.…”

Section: Introductionmentioning

confidence: 99%

Development of a single‐run liquid chromatography‐mass spectrometry analysis for the detection of 11 multiclass contaminants of emerging concern using a direct filtration method

Razak

Aris

Sukatis

et al. 2022

J of Separation Science

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A Reliable Method for Quantifying Plasma Cell-Free DNA Using an Internal Standard Strategy: Evaluation in a Cohort of Non-Pregnant and Pregnant Women

Cepeda,

Racca,

Rossetti

et al. 2023

Reprod. Sci.

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Toxicological analyses: analytical method validation for prevention or diagnosis

Cited by 5 publications

References 99 publications

Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil

Low-cost method for quantification of salicylic acid loaded in lipid nanoparticles containing copaiba oil

Development of a single‐run liquid chromatography‐mass spectrometry analysis for the detection of 11 multiclass contaminants of emerging concern using a direct filtration method

A Reliable Method for Quantifying Plasma Cell-Free DNA Using an Internal Standard Strategy: Evaluation in a Cohort of Non-Pregnant and Pregnant Women

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