volume 91, issue 25, P11797-11801 1994
DOI: 10.1073/pnas.91.25.11797
View full text
Sign up to set email alerts

Abstract: ABSTRACTThe deoxycytidine analog 5-aza-2'-deoxycytidine (5-azadCyd) has been widely used as a DNA methylation inhibitor to experimentally induce gene expression and cellular differentiation. Prior to the availability of mutant mice with altered DNA methyltransferase levels, treatment of cells with drugs has been the only means to experimentally manipulate the level of genomic DNA methylation in mammalian cells. Substitution ofDNA with 5-azadCyd leads to covalent trapping of the enzyme, thereby depleting the c…

Expand abstract