Toxic influence of pristine and surfactant modified halloysite nanotubes on phytopathogenic bacteria

Abhinayaa, R.; Jeevitha, G.; Mangalaraj, D.; Ponpandian, N.; Meena, Parmeshwar Lal

doi:10.1016/j.clay.2019.03.022

Cited by 30 publications

(14 citation statements)

References 51 publications

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“…There is no clear consensus regarding the cytotoxicity of pristine HNTs toward bacterial cells, ,− and any chemical modification of these particles may alter their behavior . Thus, we investigate the effect of the synthesized antibody-functionalized HNTs on the viability of E.…”

Section: Resultsmentioning

confidence: 99%

Antibody-Functionalized Halloysite Nanotubes for Targeting Bacterial Cells

Setter

Movsowitz

Goldberg

et al. 2021

ACS Appl. Bio Mater.

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Halloysite nanotubes (HNTs) are naturally occurring tubular clay particles which have emerged in recent years as a promising nanomaterial for numerous applications. Specifically, HNTs’ large pore volume and high specific surface area in combination with their biocompatibility make them ideal nanocarriers for bioactive compounds. This research aims to design and synthesize functionalized HNTs, which could selectively bind to target bacterial cells in suspension. Such a system can allow us to treat target cells within a challenging heterogeneous population, such as contaminated ecosystems or gut flora. HNTs functionalization is achieved by immobilizing specific antibodies onto the nanotube surface. The synthetic route is realized by the following subsequent steps: acidic etching of the HNTs, silanization of reactive surface hydroxyls, conjugation of protein A, and oriented immobilization of the antibody. HNT functionalization is studied by a set of analytical techniques including attenuated total reflectance Fourier-transform infrared spectroscopy, zeta potential measurements, thermal gravimetric analysis, scanning and transmission electron microscopy, as well as fluorescence microscopy. The selective binding of the functionalized HNTs to their target bacteria is observed upon incubation with live homogenous and heterogeneous cultures using fluorescence microscopy and high-throughput flow cytometry. Plate count and live/dead staining experiments demonstrate the biocompatibility of the antibody-HNT hybrid with its target bacteria. The suggested HNT-based smart carrier constitutes a generic platform for targeted delivery that could be selectively tailored against any microorganism by facile antibody adjustment.

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Section: Resultsmentioning

confidence: 99%

Antibody-Functionalized Halloysite Nanotubes for Targeting Bacterial Cells

Setter

Movsowitz

Goldberg

et al. 2021

ACS Appl. Bio Mater.

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“…The latter was shown by Panchal et al who have used HNTs to stabilize crude oil emulsions in sea water and demonstrated the compatibility of 5-10 mg mL −1 HNTs with the oildegrading bacteria Alcanivorax borkumensis (A. borkumensis). 87 Conversely, Abhinayaa et al 74 determined the minimum inhibitory concentration (MIC) of pristine HNTs for phytopathogenic bacteria (e.g., Agrobacterium tumifaciens and Xanthomonas oryzae) to be ∼2.5 mg mL −1 in a viability assay employing Resazurin dye. Lower concentrations of HNTs (>0.6 mg mL −1 ) inhibited to some extent bacteria growth rate and damaged cell membrane integrity, and this behaviour was ascribed to the effect of siloxane groups (on HNTs surface) in combination with the generation of reactive oxygen species (ROS).…”

Section: Hnts Origin and Propertiesmentioning

confidence: 99%

“…5. Abhinayaa et al 74 have investigated the efficacy of surfactant-modified HNTs (0.3 and 0.6 mg mL −1 ) in protection against phytopathogenic bacteria (Agrobacterium tumifaciens, Xanthomonas oryzae, and Ralstonia solanacearum) by measuring the respective MIC values, growth rate, cell membrane integrity, ROS production, and biofilm formation. Their main finding was that cetyltrimethylammonium bromide (CTAB)modified HNTs were the most potent in killing the bacteria, due to their positive zeta potential, small size and narrow size distribution, as well as their hydrophobicity.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Halloysite nanotubes – the nano-bio interface

Setter

Segal

2020

Nanoscale

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“…Additionally, the positive charges from the amino groups of the aminoclay nanoparticles at physiological pH values makes them excellent candidates for electrostatic binding with DNA (Whilton et al, 1998;Datta et al, 2013) and diffuse as a complex through an aqueous environment without substantial adverse aggregation. Certain clay mineral nanomaterials and particularly aminoclays have been reported as acting as a bactericide (Williams et al, 2011;Ito et al, 2018;Abhinayaa et al, 2019;Gaálová et al, 2019;Li et al, 2019). The bactericidal activity of aminoclays is a consequence of the easy adsorption of the aminoclays onto bacterial cell surfaces followed by penetration of the cell wall and membrane(s), leading to membrane damage, depolarization, leakage of intracellular components and cell death (Chandrasekaran et al, 2011), but cell survival can potentially be controlled by adjusting the concentration of aminoclays that the microorganisms are exposed.…”

Section: Introductionmentioning

confidence: 99%

Magnesium aminoclays as plasmid delivery agents for non-competent Escherichia coli JM109 transformation

Mendes

Kluskens

Lanceros‐Méndez

et al. 2021

Applied Clay Science

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Magnesium aminoclays were synthesized and used to transform non-competent Escherichia coli JM109 using the exogenous plasmid pUC19. The structure determined for the Mg aminoclays is analogous to 2:1 trioctahedral smectites such as talc, with an approximate composition R 8 Si 8 Mg 6 O 16 (OH) 4 , where R = CH 2 CH 2 NH 2 , morphologically arranged in layered sheets. Mg aminoclays were employed as a cationic vehicle that enabled the passage of plasmids across the cell envelope and led to genetic modification of the host. A stock solution of 10 mg/mL of Mg aminoclays was prepared, mixed with E. coli JM109 and pUC19 plasmid, and spread over Petri dishes containing lysogeny broth (LB), isopropyl β-D-1-thiogalactopyranoside (IPTG), 5-bromo-4-chloro-3indolyl-β-D-galactopyranoside (X-gal), ampicillin and various concentrations of agar (1-4%). The transformation efficiency obtained was higher for 1% and 2% agar even though transformation also occurred at agar concentrations of 3% and 4%. The optical density of E. coli JM109 and spreading time were also adjusted, favoring transformation when cells were used in their exponential growth phase (OD 600 = 1.0) and spread for 90 s. Transformation was confirmed by the growth of blue colonies in LB/IPTG/X-gal/agar Petri dishes containing ampicillin, by regrowth of biomass in liquid media containing ampicillin and by agarose gel electrophoresis of the linearized pUC19 plasmid that followed plasmidic DNA extraction from 4 blue colonies. The maximum transformation efficiency achieved was 7.0 × 10 3 CFU/μg pUC19. This transformation approach proved to be suitable for a convenient, cost-effective, room-temperature, risk-free and rapid transformation of non-competent E. coli JM109.

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Toxic influence of pristine and surfactant modified halloysite nanotubes on phytopathogenic bacteria

Cited by 30 publications

References 51 publications

Antibody-Functionalized Halloysite Nanotubes for Targeting Bacterial Cells

Antibody-Functionalized Halloysite Nanotubes for Targeting Bacterial Cells

Halloysite nanotubes – the nano-bio interface

Magnesium aminoclays as plasmid delivery agents for non-competent Escherichia coli JM109 transformation

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