Toxic essential oils. Part II: Chemical, toxicological, pharmacological and microbiological profiles of Artemisia annua L. volatiles

Radulović, Niko S.; Randjelović, Pavle J.; Stojanović, Nikola; Blagojević, Polina D.; Stojanović-Radić, Zorica; Ilić, Ivan; Djordjević, Vidosava

doi:10.1016/j.fct.2013.04.016

Cited by 88 publications

(79 citation statements)

References 54 publications

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“…Most of our findings were consistent with those reported in literature (Bilia et al, 2014;Radulovi c et al, 2013), while few data refer to the activity against S. Enteritidis and E. coli.…”

Section: Antimicrobial Activitysupporting

confidence: 95%

Antibacterial activity of Tuscan Artemisia annua essential oil and its major components against some foodborne pathogens

Donato

Santomauro

Bilia

et al. 2015

LWT - Food Science and Technology

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Recently, the attention of researchers regarding Artemisia annua has been focused on the antimicrobial activity of the essential oil. This oil, rich in mono- and sesquiterpenes, has a strong activity against some Gram-negative and Gram-positive bacteria.\ud The aim of our study is to further contribute to the knowledge of the antibacterial activity of an Artemisia annua essential oil collected in Tuscany and its three most represented compounds (artemisia ketone, 1,8-cineole and camphor).\ud The essential oil obtained by hydrodistillation gave a yield of 0.37% (w/w fresh plant material). The composition of the essential oil extracted from flowering aerial parts was determined by gas chromatography (GC/FID and GC/MS).\ud The essential oil and compounds were tested for activity against Escherichia coli O157, Salmonella Enteritidis, Salmonella Typhi, Yersinia enterocolitica and Listeria monocytogenes, all of which have great significance in foodborne infections.\ud The antibacterial activity was tested using disk diffusion method and broth microdilution assay. The microorganisms tested were all sensitive to the A. annua essential oil in toto and to all its components, which often have lower activity than oil in toto

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“…Most of our findings were consistent with those reported in literature (Bilia et al, 2014;Radulovi c et al, 2013), while few data refer to the activity against S. Enteritidis and E. coli.…”

Section: Antimicrobial Activitysupporting

confidence: 95%

Antibacterial activity of Tuscan Artemisia annua essential oil and its major components against some foodborne pathogens

Donato

Santomauro

Bilia

et al. 2015

LWT - Food Science and Technology

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“…Scopoletin, also present in A. annua, was targeted because of its immunomodulatory activity and potential indirect interaction with the parasite infection process (see review by Weathers et al, 2014a). Although artemisia ketone can be a common constituent of A. annua , often comprising >60% of the essential oil fraction (Bhakuni et al, 2001; Radulović et al, 2013), it is absent in some cultivars (Rana et al, 2013) including SAM, the one used in this study, and thus was not measured.…”

Section: 0 Results and Discussionmentioning

confidence: 93%

Changes in key constituents of clonally propagated Artemisia annua L. during preparation of compressed leaf tablets for possible therapeutic use

Weathers

Towler

2014

Industrial Crops and Products

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Artemisia annua L., long used as a tea infusion in traditional Chinese medicine, produces artemisinin. Although artemisinin is currently used as artemisinin-based combination therapy (ACT) against malaria, oral consumption of dried leaves from the plant showed efficacy and will be less costly than ACT. Many compounds in the plant have some antimalarial activity. Unknown, however, is how these plant components change as leaves are processed into tablets for oral consumption. Here we compared extracts from fresh and dried leaf biomass with compressed leaf tablets of A. annua. Using GC-MS, nineteen endogenous compounds, including artemisinin and several of its pathway metabolites, nine flavonoids, three monoterpenes, a coumarin, and two phenolic acids, were identified and quantified from solvent extracts to determine how levels of these compounds changed during processing. Results showed that compared to dried leaves, artemisinin, arteannuin B, artemisinic acid, chlorogenic acid, scopoletin, chrysoplenetin, and quercetin increased or remained stable with powdering and compression into tablets. Dihydroartemisinic acid, monoterpenes, and chrysoplenol-D decreased with tablet formation. Five target compounds were not detectable in any of the extracts of this cultivar. In contrast to the individually measured aglycone flavonoids, using the AlCl3 method, total flavonoids increased nearly fivefold during the tablet formation. To our knowledge this is the first study documenting changes that occurred in processing dried leaves of A. annua into tablets. These results will improve our understanding of the potential use of not only this medicinal herb, but also others to afford better quality control of intact plant material for therapeutic use.

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“…Although the pharmacological application of essential oils against mammalian parasitic infections remains unclear due the potential risk of toxicity, it has recently been reported that essential oils from Artemisina annua (Radulović et al, 2013), Croton argyropylloides (de Franç aNeto et al, 2012), Ligustim chuanxiong (Zhang et al, 2012) and Menta villosa (Da Silva et al, 2012) are not toxic in animal models. In addition, the present work has also demonstrated that T. capitellatus essential oil has antiLeishmania activity without significant cytotoxicity against mammalian cell lines (macrophages and endothelial cells).…”

Section: Discussionmentioning

confidence: 99%

Activity of Thymus capitellatus volatile extract, 1,8-cineole and borneol against Leishmania species

Machado

Dinis

Santos-Rosa

et al. 2014

Veterinary Parasitology

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a b s t r a c tIn the search for new leishmanicidal agents, Thymus capitellatus Hoffmanns. & Link (family Lamiaceae) volatile extract and its major compounds, 1,8-cineole and borneol, were tested against Leishmania infantum, Leishmania tropica and Leishmania major. Plant volatile extract (essential oil) was analysed by GC and GC-MS and the activity of essential oil on Leishmania promastigotes viability was assessed using tetrazolium-dye colorimetric method (MTT). The MTT test was also used to assess the cytotoxicity of essential oil on macrophages and bovine aortic endothelial cells. Effects on parasites were also analyzed by flow cytometry in order to assess mitochondrial transmembrane electrochemical gradient (JC-1), analyze phosphatidylserine externalization (annexin V-FITC, propidium iodide) and evaluate cell cycle (DNase-free, RNase, PI). Morphological and ultrastructural studies were performed by light, scanning and transmission electron microscopy. T. capitellatus volatile extract exhibited anti-parasite activity on Leishmania species, with IC 50 values ranging from 35 to 62 g/ml. However, major compounds 1,8-cineole and borneol did not showed biological activity suggesting that these monoterpenes are not responsible for the antileishmanial activity of T. capitellatus essential oil. Appearance of aberrant-shaped cells, mitochondrial swelling and autophagosomal structures were some of the ultrastructural alterations exhibited among treated promastigote cells. T. capitellatus promoted leishmanicidal effect by triggering a programmed cell death as evidenced by externalization of phosphatidylserine, loss of mitochondrial membrane potential, and cell-cycle arrest at the G(0)/G(1) phase. The volatile extract did not induced cytotoxic effects on mammalian cells. Taken together, these results suggest that T. capitellatus may represent a valuable source for therapeutic control of leishmaniasis in humans and animals.

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Toxic essential oils. Part II: Chemical, toxicological, pharmacological and microbiological profiles of Artemisia annua L. volatiles

Cited by 88 publications

References 54 publications

Antibacterial activity of Tuscan Artemisia annua essential oil and its major components against some foodborne pathogens

Antibacterial activity of Tuscan Artemisia annua essential oil and its major components against some foodborne pathogens

Changes in key constituents of clonally propagated Artemisia annua L. during preparation of compressed leaf tablets for possible therapeutic use

Activity of Thymus capitellatus volatile extract, 1,8-cineole and borneol against Leishmania species

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