Towards the Physiological Understanding of Yarrowia lipolytica Growth and Lipase Production Using Waste Cooking Oils

Abstract

The yeast Yarrowia lipolytica is an industrially relevant microorganism, which is able to convert low-value wastes into different high-value, bio-based products, such as enzymes, lipids, and other important metabolites. Waste cooking oil (WCO) represents one of the main streams generated in the food supply chain, especially from the domestic sector. The need to avoid its incorrect disposal makes this waste a resource for developing bioprocesses in the perspective of a circular bioeconomy. To this end, the strain Y. lipolytica W29 was used as a platform for the simultaneous production of intracellular lipids and extracellular lipases. Three different minimal media conditions with different pH controls were utilized in a small-scale (50 mL final volume) screening strategy, and the best condition was tested for an up-scaling procedure in higher volumes (800 mL) by selecting the best-performing possibility. The tested media were constituted by YNB media with high nitrogen restriction (1 g L−1 (NH4)2SO4) and different carbon sources (3% w v−1 glucose and 10% v v−1 WCO) with different levels of pH controls. Lipase production and SCO content were analyzed. A direct correlation was found between decreasing FFA availability in the media and increasing SCO levels and lipase activity. The simultaneous production of extracellular lipase (1.164 ± 0.025 U mL−1) and intracellular single-cell oil accumulation by Y. lipolytica W29 growing on WCO demonstrates the potential and the industrial relevance of this biorefinery model.

Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications

“…Changes in the structures of these lipases allow for improvements in their electrostatic and hydrophobic interactions, facilitating the transport of ions and favoring intracellular osmotic balance [56]. Examples of halotolerant lipases are those obtained from Y. lipolytica yeasts and Candida rugosa, which are applied in the food industry [57,58].…”

Trends in the Use of Lipases: A Systematic Review and Bibliometric Analysis

“… Network visualization map for the co-occurrence of works that bring information about the scale-up process using lipases applied to bioprocesses [ 41 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ]. Publications in the period from 2018 to 2022 were considered.…”

Response surface optimization of solvent tolerant cold active lipase production by Pseudomonas sp. VITCLP4

“…Among the non-significant variables with negative main effects, glucose was noncontributory to lipase production due to catabolite repression [36]. This is equitable with what has been observed in Pseudomonas sp., Bacillus sp., and Yarrowia lipolytica [36][37][38].…”