Towards regenerative therapy for cardiac disease

Ptaszek, Leon M.; Mansour, Moussa; Ruskin, Jeremy N.; Chien, Kenneth R.

doi:10.1016/s0140-6736(12)60075-0

Cited by 217 publications

(176 citation statements)

References 80 publications

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“…1,2 To date, various strategies have been developed to replace the scar tissue and restore cardiac functions. [3][4][5][6] However, these strategies have shown limited therapeutic efficacy in repairing the damaged myocardium. 5,6 Cardiac tissue engineering offers a promising strategy for myocardial regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…[3][4][5][6] However, these strategies have shown limited therapeutic efficacy in repairing the damaged myocardium. 5,6 Cardiac tissue engineering offers a promising strategy for myocardial regeneration. The strategy is to seed specific cells within 3-D biomaterials to reconstruct their organization into contracting cardiac tissues, later to be transplanted to damaged myocardium to restore function.…”

Section: Introductionmentioning

confidence: 99%

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Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

Sun

Zhou

Huang

et al. 2017

IJN

103

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Carbon nanotubes (CNTs) provide an essential 2-D microenvironment for cardiomyocyte growth and function. However, it remains to be elucidated whether CNT nanostructures can promote cell–cell integrity and facilitate the formation of functional tissues in 3-D hydrogels. Here, single-walled CNTs were incorporated into collagen hydrogels to fabricate (CNT/Col) hydrogels, which improved mechanical and electrical properties. The incorporation of CNTs (up to 1 wt%) exhibited no toxicity to cardiomyocytes and enhanced cell adhesion and elongation. Through the use of immunohistochemical staining, transmission electron microscopy, and intracellular calcium-transient measurement, the incorporation of CNTs was found to improve cell alignment and assembly remarkably, which led to the formation of engineered cardiac tissues with stronger contraction potential. Importantly, cardiac tissues based on CNT/Col hydrogels were noted to have better functionality. Collectively, the incorporation of CNTs into the Col hydrogels improved cell alignment and the performance of cardiac constructs. Our study suggests that CNT/Col hydrogels offer a promising tissue scaffold for cardiac constructs, and might serve as injectable biomaterials to deliver cell or drug molecules for cardiac regeneration following myocardial infarction in the near future.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

Sun

Zhou

Huang

et al. 2017

IJN

103

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“…3,4 Thus far, it has been shown that various populations of stem cells, such as bone marrow mesenchymal stem cells, 5 white adipose-derived stem cells, 6 umbilical cord blood stem cells, 7 and resident cardiac stem cells, 8 are capable of producing CMs to inhibit further deterioration and help restore cardiac function caused by ischemic heart disease. However, the differentiation ability of these stem cells into CMs was assisted by the stimulation of chemical inducers.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via β1-integrin-dependent TGF-β1 signaling pathway

Sun¹,

Mou²,

Li³

et al. 2016

IJN

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Stem cell-based therapy remains one of the promising approaches for cardiac repair and regeneration. However, its applications are restricted by the limited efficacy of cardiac differentiation. To address this issue, we examined whether carbon nanotubes (CNTs) would provide an instructive extracellular microenvironment to facilitate cardiogenesis in brown adipose-derived stem cells (BASCs) and to elucidate the underlying signaling pathways. In this study, we systematically investigated a series of cellular responses of BASCs due to the incorporation of CNTs into collagen (CNT-Col) substrates that promoted cell adhesion, spreading, and growth. Moreover, we found that CNT-Col substrates remarkably improved the efficiency of BASCs cardiogenesis by using fluorescence staining and quantitative real-time reverse transcription-polymerase chain reaction. Critically, CNTs in the substrates accelerated the maturation of BASCs-derived cardiomyocytes. Furthermore, the underlying mechanism for promotion of BASCs cardiac differentiation by CNTs was determined by immunostaining, quantitative real-time reverse transcription-polymerase chain reaction, and Western blotting assay. It is notable that β1-integrin-dependent TGF-β1 signaling pathway modulates the facilitative effect of CNTs in cardiac differentiation of BASCs. Therefore, it is an efficient approach to regulate cardiac differentiation of BASCs by the incorporation of CNTs into the native matrix. Importantly, our findings can not only facilitate the mechanistic understanding of molecular events initiating cardiac differentiation in stem cells, but also offer a potentially safer source for cardiac regenerative medicine.

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“…Considerable efforts, including clinical trials (e.g., hematopoietic stem cells used for various hematological disorders), have been performed to develop stem cell technology, but still many problems are present and need to be resolved. [22][23][24] The concept of stem cell transplantation is based on the capacity of stem cells to self-renew and regenerate tissues and organ systems. Thus, stem cell technology can be used to model and treat human ailments such as type 1 diabetes, 25 Parkinson's, 26 and cardiovascular disease.…”

mentioning

confidence: 99%

Technology Advancement for Integrative Stem Cell Analyses

Jeong

Choi

Lee

2014

Tissue Engineering Part B: Reviews

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Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose-by introducing a concept of vertical and horizontal approach-that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.

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Towards regenerative therapy for cardiac disease

Cited by 217 publications

References 80 publications

Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via β1-integrin-dependent TGF-β1 signaling pathway

Technology Advancement for Integrative Stem Cell Analyses

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Towards regenerative therapy for cardiac disease

Cited by 217 publications

References 80 publications

Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via &beta;1-integrin-dependent TGF-&beta;1 signaling pathway

Technology Advancement for Integrative Stem Cell Analyses

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Carbon nanotube-based substrates promote cardiogenesis in brown adipose-derived stem cells via β1-integrin-dependent TGF-β1 signaling pathway