Towards a load bearing hydrogel: A proof of principle in the use of osmotic pressure for biomimetic cartilage constructs

Schuiringa, Gerke H.; Pastrama, Maria Ioana; Ito, Keita; Donkelaar, Corrinus C. van

doi:10.1016/j.jmbbm.2022.105552

Cited by 3 publications

(3 citation statements)

References 54 publications

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“…Osmotic pressure sometimes causes chondrocytes to burst ( Hara et al, 2018 ) and, also be used to enhance the mechanical properties of cartilage tissue engineering grafts ( Schuiringa et al, 2023 ).…”

Section: Classification and The Influence Of Mechanical Stimulimentioning

confidence: 99%

“…Hydrogels that are frequently utilized for tissue regeneration, however, are too supple to withstand load-bearing in the joint. To address this problem, researchers have created an implant in which the osmotic pressure created by a charged hydrogel’s swelling potential, which is constrained from swelling by a textile spacer fabric, serves as the mechanical load-bearing function ( Schuiringa et al, 2023 ).…”

Section: Classification and The Influence Of Mechanical Stimulimentioning

confidence: 99%

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Double-edged role of mechanical stimuli and underlying mechanisms in cartilage tissue engineering

Jia,

Le,

Wang

et al. 2023

Front. Bioeng. Biotechnol.

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Mechanical stimuli regulate the chondrogenic differentiation of mesenchymal stem cells and the homeostasis of chondrocytes, thus affecting implant success in cartilage tissue engineering. The mechanical microenvironment plays fundamental roles in the maturation and maintenance of natural articular cartilage, and the progression of osteoarthritis Hence, cartilage tissue engineering attempts to mimic this environment in vivo to obtain implants that enable a superior regeneration process. However, the specific type of mechanical loading, its optimal regime, and the underlying molecular mechanisms are still under investigation. First, this review delineates the composition and structure of articular cartilage, indicating that the morphology of chondrocytes and components of the extracellular matrix differ from each other to resist forces in three top-to-bottom overlapping zones. Moreover, results from research experiments and clinical trials focusing on the effect of compression, fluid shear stress, hydrostatic pressure, and osmotic pressure are presented and critically evaluated. As a key direction, the latest advances in mechanisms involved in the transduction of external mechanical signals into biological signals are discussed. These mechanical signals are sensed by receptors in the cell membrane, such as primary cilia, integrins, and ion channels, which next activate downstream pathways. Finally, biomaterials with various modifications to mimic the mechanical properties of natural cartilage and the self-designed bioreactors for experiment in vitro are outlined. An improved understanding of biomechanically driven cartilage tissue engineering and the underlying mechanisms is expected to lead to efficient articular cartilage repair for cartilage degeneration and disease.

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Section: Classification and The Influence Of Mechanical Stimulimentioning

confidence: 99%

Section: Classification and The Influence Of Mechanical Stimulimentioning

confidence: 99%

Double-edged role of mechanical stimuli and underlying mechanisms in cartilage tissue engineering

Jia,

Le,

Wang

et al. 2023

Front. Bioeng. Biotechnol.

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show abstract

“…The spacer fabric can be filled with swelling hydrogels and cells, resulting in a promising platform technology to biofabricate textile‐constraining hydrogels, referred to as HydroSpacer. A recent study of Schuiringa et al 18 showed that filling with a hydrogel with strong swelling potential led to superior load‐bearing properties, mimicking not only the equilibrium stiffness but also peak stiffness and time‐dependent behavior of cartilage. The osmotic pressure generated by the swelling potential when a charged hydrogel is restricted from swelling by a textile spacer fabric, significantly increases the stiffness of the entire construct.…”

Section: Introductionmentioning

confidence: 99%

The effect of HydroSpacer implant placement on the wear of opposing and adjacent cartilage

Damen

Schuiringa

Ito

et al. 2022

Journal Orthopaedic Research

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A HydroSpacer implant, that is, a swelling hydrogel confined by a spacer fabric, was developed to repair focal cartilage defects and to prevent progression into osteoarthritis. The present study evaluated the effect of implant placement height in an osteochondral (OC) plug on wear of the opposing and adjacent cartilage. Three‐dimensional warp‐knitted spacer fabrics, polycaprolactone with poly(4‐hydroxybutyrate) pile yarns, were filled with a hyaluronic acid methacrylate and chondroitin sulfate methacrylate hydrogel. After polymerization of the hydrogel, these HydroSpacers were implanted in OC defects (ø 6 mm) created in bovine OC plugs (ø 10 mm) and allowed to swell to equilibrium. A custom‐made pin‐on‐plate wear apparatus was used to apply simultaneous compression and sliding against bovine cartilage. Cartilage damage, visualized with Indian ink, was only seen for the group in which the HydroSpacer was placed flush with the surrounding cartilage. A significant increase on average surface roughness of the sliding path compared to the adjacent cartilage confirmed surface damage for this group. When the implants were recessed (with and without extra hydrogel layer on top of the implant), this damage was not observed, but the cartilage surrounding the implants was compressed (without damage) indicating substantial load sharing with the implant. Furthermore, it was shown that all defects treated with a HydroSpacer implant resulted in shear forces comparable to intact cartilage. Clinical significance: The present study suggests that placing a HydroSpacer implant recessed into the surrounding cartilage would decrease wear of the opposing cartilage. Altogether, this study supports the development of textile‐constraining hydrogels for cartilage replacement.

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A Comprehensive Investigation of the Effect of Ag on the Structure and Antibacterial Efficacy of Li-Substituted 58s and 68s Bioactive Glasses

Rahmani

Monfared

Taherkhani

et al. 2023

Preprint

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Towards a load bearing hydrogel: A proof of principle in the use of osmotic pressure for biomimetic cartilage constructs

Cited by 3 publications

References 54 publications

Double-edged role of mechanical stimuli and underlying mechanisms in cartilage tissue engineering

Double-edged role of mechanical stimuli and underlying mechanisms in cartilage tissue engineering

The effect of HydroSpacer implant placement on the wear of opposing and adjacent cartilage

A Comprehensive Investigation of the Effect of Ag on the Structure and Antibacterial Efficacy of Li-Substituted 58s and 68s Bioactive Glasses

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