Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 5. Synthesis of the deoxyribopolynucleotide segments representing the nucleotide sequence 71-103.

Jay, Ernest; Cashion, P.; Fridkin, Mati; Ramamoorthy, B.; Agarwal, Kan; Caruthers, Marvin H.; Khorana, H G

doi:10.1016/s0021-9258(17)33830-9

Cited by 23 publications

(2 citation statements)

References 11 publications

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“…Segment 4 was a decanucleotide containing 50% deoxyguanylic acid. As demonstrated by previous syntheses (Jay et al, 1976), guanine rich sequences are difficult to prepare. The condensations usually proceed to a lower extent than with other nucleotides.…”

Section: Resultsmentioning

confidence: 97%

“…The condensations usually proceed to a lower extent than with other nucleotides. Furthermore, the isobutyryl blocking group is somewhat unstable to purification procedures involving TEAB on DEAE-cellulose (Jay et al, 1976). In order to minimize the latter problem, dinucleotide blocks which were used to synthesize this sequence were reisobutyrylated immediately before use.…”

Section: Resultsmentioning

confidence: 99%

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Studies on gene control regions. 4. Synthesis and biological activity of a λ pseudo operator

Kawashima¹,

Gadek²,

Mh³

1977

Biochemistry

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The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six proposed operator sites in the leftward and rightward operators. However, it does not correspond exactly to any single proposed operator sequence. The chemical synthesis includes the deoxyoligonucleotides d(T-A-T-C-A-C), d(C-G-C-C-G-G-T-G-A-T-A), d(T-A-T-C-A-C-C), and d(G-G-C-G-G-T-G-A-T-A). These deoxyoligonucleotides were joined with T4 DNA ligase to form d(T-A-T-C-A-C-C-G-C-C-G-G-T-G-A-T-A) and d(T-A-T-C-A-C-C-G-G-C-G-G-T-G-A-T-A). The cI repressor protein was found to bind to the duplex formed from these two segments.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Studies on gene control regions. 4. Synthesis and biological activity of a λ pseudo operator

Kawashima¹,

Gadek²,

Mh³

1977

Biochemistry

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Gensynthese

Engels

Uhlmann²

1989

Angewandte Chemie

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Oligonucleotid-Synthese, bis vor einigen Jahren noch eher ein exotisches Betatigungsfeld weniger Experten, ist mittlerweile integraler Bestandteil des Arsenals molekularbiologischer Techniken. Gerade im letzten Jahrzehnt hat sich auf dem Gebiet der DNA-Synthese eine unglaublich rasante Entwicklung abgespielt, welche zur Automatisierung der Synthesen fiihrte und schlieBlich in der Herstellung von Genen mit einer Lange von iiber 1000 Basenpaaren gipfelte. MaBgeschneiderte synthetische Gene ermoglichen heute auch die gentechnische Herstellung veranderter und sogar vollig neuartiger Proteine (,,de-novo-Protein-Design"). Im Zusammenspiel mit den modernen Methoden der Isolierung, Sequenzierung und Expression von Genen trug die Gensynthese in den letzten Jahren wesentlich zum enormen Fortschritt in der Gentechnologie bei. Synthesemethoden fur OligonucleotideDie Methoden zur Synthese definierter Oligonucleotide unterscheiden sich im wesentlichen durch die Art, wie die Phosphorsaureesterbindung erhalten wird. Je nach Art Angew Chem. 101 (1989) 733-752 C VCH VerlugsgeseIlsc.hafi mbH, 0-6940 Weinhrim, I989

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Gene Synthesis [New Synthetic Methods (77)]

Engels

Uhlmann

1989

Angew. Chem. Int. Ed. Engl.

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Oligonucleotide synthesis, until a few years ago the rather exotic preserve of a few experts, has become an integral part of the arsenal of molecular‐biological techniques. The last decade, in particular, has seen unbelievably rapid development in this area. DNA synthesis has been automated and can now produce genes greater than 1000 base pairs in length. Tailor‐made synthetic genes also permit the synthesis of altered or even novel proteins (de novo protein design) by gene‐technological methods. Together with modern methods of gene isolation, sequencing, and expression, gene synthesis has played a major part in the enormous advances achieved in gene technology.

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Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 5. Synthesis of the deoxyribopolynucleotide segments representing the nucleotide sequence 71-103.

Cited by 23 publications

References 11 publications

Studies on gene control regions. 4. Synthesis and biological activity of a λ pseudo operator

Studies on gene control regions. 4. Synthesis and biological activity of a λ pseudo operator

Gensynthese

Gene Synthesis [New Synthetic Methods (77)]

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