Total phenolic, flavonoid content and metabolite profiling of methanol extract of date (Phoenix dactylifera) seeds by LC-QTOF-MS

Warnasih, Siti; Salam, Supriatno; Hasanah, Uswah; Ambarsari, Laksmi; Sugita, Purwantiningsih

doi:10.1063/5.0001436

Cited by 6 publications

(12 citation statements)

References 33 publications

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“…Phoenix dactylifera has antihyperglycemic, anticancer, anti-hypertensive, anti-hyperlipidemic, antimicrobial, antioxidant, anti-inflammatory, neuroprotective fertilityenhancing, gastroprotective, immunoprotective, and hepatoprotective properties, as well as antiviral properties [4,5]. Cholesterol Sterol [10,11]…”

Section: Resultsmentioning

confidence: 99%

“…Phenolic acid [11,12] Gallic Acid Phenolic acid [13,14] Ferulic Acid Phenolic acid [11,[13][14][15] Chlorogenic Acid Phenolic acid [13][14][15] p-Hidroxybenzoic Acid Phenolic acid [13][14][15] Caffeic acid Phenolic acid [11][12][13]15] Proanthocyanidin B1 Polyphenol [12] Proanthocyanidin B2 Polyphenol [12] Protocatechuic acid Phenolic acid [12,13] Compound Chemical Structure Type Ref.…”

Section: -O-caffeoylshikimic Acidmentioning

confidence: 99%

“…Phenolic acid [13] Hydrocaffeic acid Phenolic acid [12] p-coumaric acid Phenolic acid [11,[13][14][15] Quercetin Flavonoid [12][13][14][15] Chelidonic acid Phenolic acid [15] Pyrogallol Phenolic [14]…”

Section: Syringic Acidmentioning

confidence: 99%

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Molecular Docking of Secondary Metabolites from Phoenix dactylifera L. Targeting SARS-CoV-2 ACE-2 Receptor

Warnasih

2023

J. Kartika Kimia

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Dates (Phoenix dactylifera L.) are one of the most useful plants. According to previous research, those dates contain bioactive compounds with antioxidant, antimicrobial, and anti-inflammatory properties. Furthermore, dates can be used as an antiviral, but the potential of dates as an antiviral, particularly the SARS-CoV-2 antiviral, has received little attention. As a result, the goal of this study was to see if date palm bioactive compounds could be used as SARS-CoV-2 antivirals with molecular docking against the Angiotensin-Converting Enzyme-2 (ACE-2) receptor. The study was carried out using molecular docking methods with Autodock Vina, where 21 chemical compounds found in date palms as ligands were attached to the ACE-2 receptor, and four chemical compounds used as SARS-CoV-2 antiviral medicines, namely chloroquine, favipiravir, plitidepsin, and remdesivir, were used as a control ligand. The results showed that proanthocyanidin B1, B2, and quercetin ligands had the highest interaction and stability with Gibbs free energy values of -9.20 kcal/mol, -9.10 kcal/mol, and -8.20 kcal/mol, respectively. This value is known to be higher than control ligands plitidepsin -9.02 kcal/mol, remdesivir -7.88 kcal/mol, chloroquine -5.65 kcal/mol, and favipiravir -5.38 kcal/mol. As a result, dates can be used as a SARS-CoV-2 antiviral candidate.

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Section: Resultsmentioning

confidence: 99%

Section: -O-caffeoylshikimic Acidmentioning

confidence: 99%

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Molecular Docking of Secondary Metabolites from Phoenix dactylifera L. Targeting SARS-CoV-2 ACE-2 Receptor

Warnasih

2023

J. Kartika Kimia

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“…These values are still far below the total phenolic values obtained in the present work, which are above 245 mg/100 g. Liu et al [ 10 ] improved phenol extraction after grinding date seeds using a supercritical fluid extraction of up to 441.57 mg of total phenolics/100 g fresh weight. Nevertheless, other authors quantify higher amounts of total phenols and flavonoids, improving not only the specific surface area by grinding below 1.5 mm, but also by making sequential extractions, up to four times, with solvents such as water, ethanol, methanol, acetone, or their mixtures [ 7 , 11 ], where the maximum amounts of total phenols and flavonoids were between 11 and 3 g/100 g of date seed, respectively, although the total phenolic acid content did not exceed 194 mg/100 g. While some of these values are much higher than those obtained in this work, applying a thermal treatment with steam without grinding the seed seems a more easily scalable solution at the industrial level, and more so considering a subsequent concentration step through adsorption resins. This makes it easier and cheaper to obtain an extract that is rich in phenols and has high antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

“…The extraction of oil is an important starting point for its industrialization [ 6 ] followed by the extraction of bioactive components such as phenols. In this regard, mainly acid phenols and flavonoids have been identified, which have been extracted with different solvents after a grinding phase, proving their antioxidant [ 5 , 7 , 8 , 9 , 10 , 11 ] and anti-inflammatory activities [ 12 ], among others.…”

Section: Introductionmentioning

confidence: 99%

Date Seed: Rich Source of Antioxidant Phenolics Obtained by Hydrothermal Treatments

Mrabet

Jiménez-Araujo²,

Fernández-Prior³

et al. 2022

Antioxidants

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The growing interest in natural compounds is helping to improve the management of agro-industrial by-products such as the date seed as sources of such compounds. In this work, the application of a hydrothermal treatment at 160 and 180 °C for 60 min was studied to achieve the solubilization of its phenolic components and sugars in order to obtain biologically active extracts. The percentage of phenols and total sugars in the final extracts were very similar, at 45 and 25% for the 160 and 180 °C treatments, respectively. The treatment at a higher temperature allowed greater solubilization of other components. The antioxidant activity was measured as free-radical scavenging capacity. For the DPPH• method, expressed as EC50, the results were 0.34 and 0.37 mg/L, the TEAC values for the ABTS• method were 6.61 and 3.28 mg/g dried extract, and the values obtained by the ORAC method were 12.82 and 9.91 mmol Trolox/g dried extract, for 160 and 180 °C, respectively. All these values are higher than those of other plant extracts and extracts obtained using the whole date. Therefore, the date seed is a very important source of phenols, and through thermal and chromatographic processes, it is possible to obtain extracts with high antioxidant activity.

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Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds

Elsherif,

Safhi,

Khalifa

et al. 2024

BMC Plant Biol

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Background Plants are considered the primary source of many principal bioactive compounds that have been utilized in a wide range of applications including the pharmaceutical and biotechnological industries. Therefore, there is an imperative need to modulate the production of natural bioactive components. The present study aimed to determine the importance of dried and pulverized date palm seeds (DPS) as a natural elicitor for the synthesis of secondary metabolites in Lotus arabicus L. Results The presence of various antioxidant compounds, simple sugars, amino acids, fatty acids and reasonable mineral contents was distinct in the phytochemical characterization of DPS. The major components detected in DPS analysis were the 5-(hydroxymethyl) furfural and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyranone. The induced callus of L. arabicus (seven weeks old) was supplemented with DPS at different concentrations (0, 2, 4, 8 and 10 g/l) in culture media. Treatment with 8 g/l DPS induced the highest antioxidant capacity, ascorbic acid content and secondary metabolites (total phenolics and flavonoids) in the produced callus. Stress biomarkers (hydrogen peroxide and malondialdehyde) were found in the control ranges except at 10 g/l DPS. The expression patterns of key genes involoved in secondary metabolism modulation, such as phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavonol synthase (FLS) and deoxyxylulose phosphate reductoisomerase (DXR), were triggered after DPS treatments. Moreover, the quantitative profiling of phenolic and flavonoid compounds showed that supplementation with DPS, especially at 8 g/l, led to pronounced increases in most of the measured compounds. Conclusion The marked upregulation of eliciting-responsive genes and overproduction of secondary metabolites provide molecular-based evidence for intensifying the principal pathways of phenylpropanoid, flavonoid and terpenoid biosynthesis. Overall, the present in vitro study highlights the stimulating capacity of DPS utilization to improve the bioactive components of L. arabicus at the physiological and molecular levels, enhancing its potential as a medicinal herb.

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Total phenolic, flavonoid content and metabolite profiling of methanol extract of date (Phoenix dactylifera) seeds by LC-QTOF-MS

Cited by 6 publications

References 33 publications

Molecular Docking of Secondary Metabolites from Phoenix dactylifera L. Targeting SARS-CoV-2 ACE-2 Receptor

Molecular Docking of Secondary Metabolites from Phoenix dactylifera L. Targeting SARS-CoV-2 ACE-2 Receptor

Date Seed: Rich Source of Antioxidant Phenolics Obtained by Hydrothermal Treatments

Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds

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