Total Phenolic Content and in Vitro Analysis of Antioxidant, Antibacterial, and Alpha-Glucosidase Inhibition Properties of Chroococcus Minutus (Kützing) Nägeli (Chroococcales, Cyanobacteria)

Abstract

Objective: Cyanobacteria are novel sources of bioactive substances with important biological activities. In this study, the total phenolic content (TPC) and bioactive (antioxidant, antidiabetic, and antibacterial) properties of a freshwater cyanobacteria, Chroococcus minutus were investigated.Material and Method: Methanol extract of C. minutus were prepared and subjected to different biological assays to determine the TPC, antioxidant, and alpha-glucosidase inhibition properties. The antibacterial activity was done using microtiter plate dilution assay against various medically important bacterial pathogens.Result and Discussion: C. minutus has a TPC of 11.27 ± 0.14 mg GAE/g. Antioxidant efficiency of C. minutus are characterized by having potent DPPH scavenging activity with IC50 value of 255 μg/ml. Assessment of alpha-glucosidase inhibition property showed that C. minutus extract have potent inhibition activity with IC50 of 5.50 μg/ml as compared to acarbose (standard antidiabetic drug). In addition, C. minutus extract exhibited potent antibacterial activities against Bacillus cereus, Listeria monocytogenes, and Methicillin-resistant Staphylococcus aureus. The current investigation shows the potential of C. minutus as source of active metabolites with important use in pharmaceutical applications.

Chemical Composition and in Vitro Analysis of Antibacterial Activity of Acutodesmus Dimorphus (Turpin) P.M. Tsarenko (Scenedesmaceae, Chlorophyta)

“…Thus, confirming the potential application of this microalgae as good source of important microelements that can be use in industrial and pharmaceutical application. Other important micro-elements like calcium, iron, and potassium were also observed in high amounts showing the potential use of A. dimorphus as additives to improve food quality needed to address malnutrition that is timely and relevant to several countries in the world [1,2,14].…”

“…Green microalgae are named for their grass-green chloroplasts with cell walls that are mostly cellulosic and may exist in several forms such as unicellular, filamentous, colonial and sheet-like. These microalgae are fast growing and are known to have diverse bioactive compounds and macromolecules that are yet to be discovered for biotechnological use, and the characterization as well as isolation of microalgae with the potent bioactive compounds remain the focus of continuing research [1,2]. The capability of several microalgal strains to survive and thrive in extreme environmental stresses results in the formation of a variety of secondary bioactive metabolites, important in agriculture and food industries [1,3].…”

“…These microalgae are fast growing and are known to have diverse bioactive compounds and macromolecules that are yet to be discovered for biotechnological use, and the characterization as well as isolation of microalgae with the potent bioactive compounds remain the focus of continuing research [1,2]. The capability of several microalgal strains to survive and thrive in extreme environmental stresses results in the formation of a variety of secondary bioactive metabolites, important in agriculture and food industries [1,3]. These active metabolites are produced by these algae to maintain cell signaling pathways and constant intracellular membrane functions in response to sudden environmental changes.…”

“…; carotenoids in Nanochloropsis oculata; and bioactive peptide in green microalga, Chlorella ellipsoidea [4][5][6]. Bioactive metabolites aid microalgal cells in their ecological interactions with their environment, including cell to cell signaling and protection against competitors and predators [1,4]. Metabolites such as phenolics, fatty acids, glycolipids, diketone, terpenes, and alkaloids derived from green microalgae are reported to exhibit antibacterial activities [1,4,8].…”

“…Bioactive metabolites aid microalgal cells in their ecological interactions with their environment, including cell to cell signaling and protection against competitors and predators [1,4]. Metabolites such as phenolics, fatty acids, glycolipids, diketone, terpenes, and alkaloids derived from green microalgae are reported to exhibit antibacterial activities [1,4,8]. These metabolites have been a valuable renewable source in the development of novel pharmaceuticals, such as anti-cancer, antibiotic, and antiinflammatory drugs.…”