Topographical Micropatterning of Poly(dimethylsiloxane) Using Laminar Flows of Liquids in Capillaries

Takayama, S.; Ostuni, Emanuele; Qian, Xiangping; McDonald, J. Cooper; Jiang, Xingyu; LeDuc, Philip; Wu, Min; Ingber, Donald E.; Whitesides, George M.

doi:10.1002/1521-4095(200104)13:8<570::aid-adma570>3.0.co;2-b

Cited by 128 publications

(83 citation statements)

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“…Laminar flows form within microchannels because of low convective mixing, which limits molecular transport to diffusion. Laminarly flowing fluids have been used to pattern cells and their microenvironments (79,80). Furthermore, an individual cell can be exposed to multiple microenvironments simultaneously by being placed at the interface between two or more adjacent streams (81).…”

Section: Microfluidics For Regulating Cell-solublementioning

confidence: 99%

Microscale technologies for tissue engineering and biology

Khademhosseini

Langer

Borenstein

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,436

1,106

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Microscale technologies are emerging as powerful tools for tissue engineering and biological studies. In this review, we present an overview of these technologies in various tissue engineering applications, such as for fabricating 3D microfabricated scaffolds, as templates for cell aggregate formation, or for fabricating materials in a spatially regulated manner. In addition, we give examples of the use of microscale technologies for controlling the cellular microenvironment in vitro and for performing high-throughput assays. The use of microfluidics, surface patterning, and patterned cocultures in regulating various aspects of cellular microenvironment is discussed, as well as the application of these technologies in directing cell fate and elucidating the underlying biology. Throughout this review, we will use specific examples where available and will provide trends and future directions in the field. microfabrication ͉ review ͉ soft lithography ͉ stem cells ͉ microfluidics

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Section: Microfluidics For Regulating Cell-solublementioning

confidence: 99%

Microscale technologies for tissue engineering and biology

Khademhosseini

Langer

Borenstein

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

1,436

1,106

View full text Add to dashboard Cite

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“…In conventional laminar flow etching, convection-dominated confinement of small-molecule chemical etchants is only possible at high flow rates (Pe $ 5 Â 10 3 for HF etching of glass at u ¼ 0.1 m/s). [12][13][14] In contrast, enzymatic machining employs macromolecular species that display greatly reduced diffusivities, enabling high Pe to be maintained at low flow rates (Pe $…”

Section: Resultsmentioning

confidence: 99%

Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies

et al. 2016

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The ability to harness enzymatic activity as an etchant to precisely machine biodegradable substrates introduces new possibilities for microfabrication. This flow-based etching is straightforward to implement, enabling patterning of microchannels with topologies that incorporate variable depth along the cross-sectional dimension. Additionally, unlike conventional small-molecule formulations, the macromolecular nature of enzymatic etchants enables features to be precisely positioned. Here, we introduce a kinetic model to characterize the enzymatic machining process and its localization by co-injection of a macromolecular inhibitor species. Our model captures the interaction between enzyme, inhibitor, and substrate under laminar flow, enabling rational prediction of etched microchannel profiles so that cross-sectional topologies incorporating complex lateral variations in depth can be constructed. We also apply this approach to achieve simultaneous widening of an entire network of microchannels produced in the biodegradable polymeric substrate poly(lactic acid), laying a foundation to construct systems incorporating a broad range of internal cross-sectional dimensions by manipulating the process conditions. Published by AIP Publishing. [http://dx

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“…Cell shapes related to growth, gene expression, extracellular matrix metabolism, and differentiation have been studied by cytometric and chemical analysis of the visco-elastic behavior of the red blood cell membrane while the cells were flowing in microfluidic channels [260].…”

Section: Cell Analysismentioning

confidence: 99%

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

2012

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This paper reviews microfluidic technologies with emphasis on applications in the fields of pharmacy, biology, and tissue engineering. Design and fabrication of microfluidic systems are discussed with respect to specific biological concerns, such as biocompatibility and cell viability. Recent applications and developments on genetic analysis, cell culture, cell manipulation, biosensors, pathogen detection systems, diagnostic devices, high-throughput screening and biomaterial synthesis for tissue engineering are presented. The pros and cons of materials like polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polystyrene (PS), polycarbonate (PC), cyclic olefin copolymer (COC), glass, and silicon are discussed in terms of biocompatibility and fabrication aspects. Microfluidic devices are widely used in life sciences. Here, commercialization and research trends of microfluidics as new, easy to use, and cost-effective measurement tools at the cell/tissue level are critically reviewed.

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Topographical Micropatterning of Poly(dimethylsiloxane) Using Laminar Flows of Liquids in Capillaries

Cited by 128 publications

References 0 publications

Microscale technologies for tissue engineering and biology

Microscale technologies for tissue engineering and biology

Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies

Polymer-Based Microfluidic Devices for Pharmacy, Biology and Tissue Engineering

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