Topical interleukin-8 antibody attracts leukocytes in a piglet lavage model

Ankermann, Tobias; Wiemann, Tina; Reisner, Anja; Orlowska-Volk, Marzenna; Köhler, Heike; Krause, Martín

doi:10.1007/s00134-004-2464-5

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…Depending on the kind of acute lung injury the increasing pre-/post-injury factor varies largely between 1:2 and 1: 60 (Table 10). IL-8 concentrations rose from 51 ± 34 pg/ml to 429 ± 259 in a lavage model (Ankermann et al, 2005b), and from 406 ± 364 pg/ml to 4,837 ± 1,951 in an meconium aspiration model (Angert et al, 2007), whereas IL-6 from BALF came up from 0.4 ± 1.0 U/ml to 29 ± 28 following repeated airway lavage (Krause et al, 2005). LTB 4 as an important chemokine in the inflamed lung and increased from 2.6 ± 1.9 pg/ml to 9.3 ± 7.8 in a newborn lavage model (Ankermann et al, 2005a).…”

Section: Resultsmentioning

confidence: 99%

“…In this context it is surprising that many aspects of dampening the innate immune system in the overwhelming response of the neonatal lung secondary to repeated airway lavage have not been studied yet (Table 12). While the early studies considering immunologic aspects measured protein content in BALF, histopathology scores, and the concentrations of TNF-α, LTB 4 , IL-1β, and MPO as a surrogate parameter for neutrophil infiltration (Sood et al, 1996a; Merz et al, 2002; van Kaam et al, 2005), more recent studies put light on the selective inhibition of NF-κB (von Bismarck et al, 2007), IL-1β metabolism (Chada et al, 2008), general immune suppression by dexamethasone or budenoside (von Bismarck et al, 2009; Yang et al, 2010), eicosanoid suppression (Ankermann et al, 2006), and blockade of IL-8 (Ankermann et al, 2005b). The impact of the ceramide metabolism in nARDS has been investigated (Barnes, 2004; von Bismarck et al, 2008; Spengler et al, 2018), and the important role of NLRP3 (nucleotide-binding domain, leucine-rich repeat-containing protein-3) in a triple-hit lung injury model has been studied (Dos Santos et al, 2012; Spengler et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Limiting damage and repair of lung tissue by the newborn organism's innate immunity without completely uncoupling the means of defense—especially in case of infectious pathogens—seems to be the distinguished task for future research by the use of the piglet lavage/meconium aspiration model. Considering the complexity of the innate immunity as very shortly outlined above, many approaches are possible but should probably tackle major anti-inflammatory pathways instead of single—even important—molecules to overcome the phenomenon of redundant activation of e.g., many cytokines [such as blocking IL-8 by specific antibodies may upregulate IL-8 production in experimental NARDS (Ankermann et al, 2005b)].…”

Section: Resultsmentioning

confidence: 99%

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An Unsettled Promise: The Newborn Piglet Model of Neonatal Acute Respiratory Distress Syndrome (NARDS). Physiologic Data and Systematic Review

2019

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Despite great advances in mechanical ventilation and surfactant administration for the newborn infant with life-threatening respiratory failure no specific therapies are currently established to tackle major pro-inflammatory pathways. The susceptibility of the newborn infant with neonatal acute respiratory distress syndrome (NARDS) to exogenous surfactant is linked with a suppression of most of the immunologic responses by the innate immune system, however, additional corticosteroids applied in any severe pediatric lung disease with inflammatory background do not reduce morbidity or mortality and may even cause harm. Thus, the neonatal piglet model of acute lung injury serves as an excellent model to study respiratory failure and is the preferred animal model for reasons of availability, body size, similarities of porcine and human lung, robustness, and costs. In addition, similarities to the human toll-like receptor 4, the existence of intraalveolar macrophages, the sensitivity to lipopolysaccharide, and the production of nitric oxide make the piglet indispensable in anti-inflammatory research. Here we present the physiologic and immunologic data of newborn piglets from three trials involving acute lung injury secondary to repeated airway lavage (and others), mechanical ventilation, and a specific anti-inflammatory intervention via the intratracheal route using surfactant as a carrier substance. The physiologic data from many organ systems of the newborn piglet—but with preference on the lung—are presented here differentiating between baseline data from the uninjured piglet, the impact of acute lung injury on various parameters (24 h), and the follow up data after 72 h of mechanical ventilation. Data from the control group and the intervention groups are listed separately or combined. A systematic review of the newborn piglet meconium aspiration model and the repeated airway lavage model is finally presented. While many studies assessed lung injury scores, leukocyte infiltration, and protein/cytokine concentrations in bronchoalveolar fluid, a systematic approach to tackle major upstream pro-inflammatory pathways of the innate immune system is still in the fledgling stages. For the sake of newborn infants with life-threatening NARDS the newborn piglet model still is an unsettled promise offering many options to conquer neonatal physiology/immunology and to establish potent treatment modalities.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

An Unsettled Promise: The Newborn Piglet Model of Neonatal Acute Respiratory Distress Syndrome (NARDS). Physiologic Data and Systematic Review

2019

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“…One of the key events of lung infl ammation is the migration of polymorphonuclear leukocytes (PMNL), which are known to release a variety of enzymes in the lung and to induce damage to type I and II pneumocytes, functional compromise of the surfactant, lung edema, and epithelial necrosis. Among the mediators of chemotactic attraction for PMNL, interleukin (IL)-8 is probably be the most potent chemoattractant factor, and elevation of IL-8 was demonstrated in lung injuries induced by both LAV [17] and oleic acid [18].…”

Section: Introductionmentioning

confidence: 99%