Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation

Bralley, Eve E; Greenspan, Phillip; Hargrove, James L.; Wicker, Louise; Hartle, Diane K.

doi:10.1186/1476-9255-5-1

Cited by 104 publications

(87 citation statements)

References 44 publications

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“…Medicinal plants have been identified as a safe and effective alternative for the treatment of dermatological inflammatory diseases, replacing conventional drugs such as corticosteroids and nonsteroidal anti-inflammatory drugs (Bralley et al 2008;Maldini et al 2009;Cesca et al 2012). In this study, we identified polar compounds in S. trilobata hydroethanolic extract and also developed a new topical phytomedicine based on KA content with significant anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 98%

Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

Fucina¹,

Rocha²,

Silva³

et al. 2016

Pharmaceutical Biology

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Context: The aerial parts of Sphagneticola trilobata (L.) Pruski (Asteraceae) are popularly used to treat topical inflammation, but have not been fully investigated. Objective: To identify polar compounds in S. trilobata extracts and develop a new topical phytomedicine based on the kaurenoic acid (KA) content while monitoring and demonstrating its topical anti-inflammatory activity. Materials and methods: Ethanol spray-dried extract of S. trilobata was analysed by LC-MS while the KA content from semisolid was analysed by LC-UV. The extent of ear edema induced by applying 20 lL of croton oil (2.5%), arachidonic acid (AA; 2 mg/ear) and decanoylphorbol-13-acetate (TPA; 2.5 mg/ear) in mice was used to evaluate the biological activity of the semisolids, which were applied 30 min before the phlogistic agents. Results: Eight phenylpropanoids and four oleanane-type triterpenoid saponins were identified, majority of them reported for the first time in this species, in addition to KA. The semisolid containing 1.0% of dried extract reduced the ear edema induced by croton oil [77.2 6 4.5%; ID50 ¼ 0.49 (0.28-0.87%)], TPA (81.5 6 2.4%) and AA (39.1 6 6.9%), with decreasing effect at higher KA concentrations. This was accompanied by neutrophil migration inhibition as investigated by biochemical and histological assays. Discussion and conclusion: The anti-inflammatory effects were (at least in part) due to the interference in protein kinase C (PKC) activation, AA-cascade products and neutrophil migration inhibition, demonstrating the efficacy of the folk topical usage of this plant. The results support the development of a novel topical anti-inflammatory phytomedicine properly standardized to treat inflammatory dermatological diseases. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 98%

Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

Fucina¹,

Rocha²,

Silva³

et al. 2016

Pharmaceutical Biology

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“…For example, Japanese knotweed (Polygonum cuspidatum) is used as an analgesic, antipyretic, diuretic and expectorant remedy in traditional Chinese medicine; anti-inflammatory properties, such as the inhibition of NF-B, have also been reported for this plant [31]. Indeed, interest in Japanese knotweed has increased due to the high concentration of resveratrol and its glycosides in the roots.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Study of the Anti-Inflammatory Activity of Some Medicinal and Edible Plants Growing in Russia

2013

JCPRM

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The effects of the ethanolic extracts of 105 plants used in Russian traditional medicine, 26 vegetables and fruits and 2 mushrooms on the release of tumor necrosis factor (TNF-) and prostaglandin E 2 (PGE 2 ) in lipopolysaccharide (LPS)-stimulated differentiated human acute monocytic leukemia THP-1 cells were studied using TNF-and PGE 2 assays, respectively. We found that 16 plant extracts inhibited TNF-production and 15 extracts decreased PGE 2 release in the cells in a concentration-dependent manner. Guilder rose, tansy, shrubby cinquefoil, wintergreen and prince's pine, the last two of which belong to the Pyrolaceae family, notably inhibited the levels of both of the inflammatory mediators.

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“…33 Bralley et al reported that grape phenolic components inhibit inflammation, edema, and white blood infiltration in mice induced with 12-O-tetra decanoylphorbol 13-acetate. 34 Additionally, this component shows comparable effects to indomethacin, a widely used medication for degenerative joint diseases. 35 These observations demonstrate that resveratrol has clear anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Inhibits Inflammation Induced by Heat-Killed Listeria monocytogenes

Park

Kim

Chin

et al. 2012

Journal of Medicinal Food

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Resveratrol is a polyphenolic compound in red wine that has antioxidant and cardioprotective effects in animal models. Listeria monocytogenes is a pathogen that mainly affects immunocompromised individuals and is initially detected at the cell surface or in phagosomes by toll-like receptor 2. Many antioxidants also exert anti-inflammatory activities; therefore, we evaluated the anti-inflammatory properties of resveratrol by studying the various inflammatory responses induced by heatkilled L. monocytogenes (HKLM). Resveratrol strongly blocked HKLM-induced NADPH oxidase-1 mRNA and reactive oxygen species production by macrophages. Resveratrol also suppressed monocyte chemotactic protein-1 expression, cyclooxygenase-2 expression, prostaglandin production, inducible nitric oxide (NO) synthase expression, and NO production induced by HKLM. We investigated the signaling pathway involved in the resveratrol effect. HKLM stimulated glycogen synthase kinase 3b (GSK3b) and extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. The involvement of GSK3b and ERK1/2 was tested using inhibitors. While the GSK3b inhibitor LiCl potentiated the effect of HKLM, the MEK inhibitor U0126 blocked these responses. Additionally, pretreatment with resveratrol blocked phosphorylation of both kinases induced by HKLM. These results suggest that HKLM is strong inducer of inflammatory mediators, and that the inhibitory effect of resveratrol may be mediated by the GSK3b and ERK1/2 pathways.KEY WORDS: COX-2 HKLM iNOS MCP-1 NADPH oxidase 1 resveratrol

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Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation

Abstract: Background: This study tested the ability of a characterized extract of Polygonum cuspidatum (PCE) to inhibit mouse ear inflammation in response to topical application of 12-Otetradecanoylphorbol-13-acetate (TPA).

Cited by 104 publications

References 44 publications

Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

Topical anti-inflammatory phytomedicine based onSphagneticola trilobatadried extracts

In Vitro Study of the Anti-Inflammatory Activity of Some Medicinal and Edible Plants Growing in Russia

Resveratrol Inhibits Inflammation Induced by Heat-Killed Listeria monocytogenes

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