Tong-fu-li-fei decoction exerts a protective effect on intestinal barrier of sepsis in rats through upregulating ZO-1/occludin/claudin-1 expression

Chen, Li; Li, Lan; Han, Ying‐Hao; Lv, Bo; Zou, Suzhao; Yu, Qian

doi:10.1016/j.jphs.2020.02.009

Cited by 37 publications

(22 citation statements)

References 9 publications

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“…Improved expression of three crucial proteins can enhance the intestinal barrier function for decreasing permeability of the intestinal wall [45]. Several studies have found that traditional Chinese medicine can alter intestinal permeability dependent on tight junctions protein changes [46,47]. Our results also demonstrated that ZO-1 and occludin mRNA expression in jejunum and ileum were increased in piglets fed MCR (8000 mg/kg feed) diet.…”

Section: Discussionsupporting

confidence: 66%

Dietary Moutan Cortex Radicis Alters Serum Antioxidant Capacity, Intestinal Immunity and Colonic Microbiota in Weaned Piglets

Bai

Liu

Wang

et al. 2020

Preprint

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Background: Moutan cortex radicis (MCR), as a common traditional Chinese medicine, has been widely used as antipyretic, antiseptic and anti-inflammatory agent in China. However, few studies have evaluated the positive effects of MCR, as a new feed additives, on alleviating weaning stress and improving intestinal health and microbiom in pigs. This study aimed to investigate the effect of dietary MCR supplementation on serum antioxidant capacity, intestinal morphology, anti-inflammatory mechanism, and microbiota in weaned piglets.Results: Supplemental 2000 mg/kg and 4000 mg/kg MCR increased (P < 0.05) the final body weight, ADG and ADFI of weaned piglets, and 2000 mg/kg MCR diet significantly decreased (P < 0.05) the F/G ratio and increased (P < 0.05) serum catalase activity compared with CON group. Also, the villus height and crypt depth in the ileum and the concentrations of total SCFA, acetic acid, butyric acid and valeric acid in the colonic contents were higher (P < 0.05) in the 2000 mg/kg and 4000 mg/kg MCR diets than CON group. Dietary MCR supplementation at 4000 mg/kg MCR significantly increased (P < 0.05) total antioxidative capability and the crypt depth in the jejunum but decreased (P < 0.05) the mRNA expression levels of Interferon γ, tumor necrosis factor-α, interleukin-1β, inhibiting kappa B kinase β (IKKβ), inhibiting nuclear factor kappa-B (IκBα) and nuclear factor kappa-B (NF-κB) in the jejunum and ileum. Supplemental 8000 mg/kg MCR had the higher total antioxidative capability and catalase activity in the serum but decreased (P < 0.05) the villus height and crypt depth in the jejunum compared with the CON group. MCR addition reduced (P < 0.05) serum malondialdehyde content, and tended to increase the mRNA expression of zonula occludens-1 in the ileum (P = 0.066) compared to the CON group. Microbiota sequencing identified the microbial richness indices (Chao1, ACE, and observed species), the relative abundances of Firmicutes and Lactobacillus were increased (P < 0.05), and the relative abundances of Bacteroides, Parabacteroides, unidentified_Lachnospiraceae and Enterococcus were reduced (P < 0.05) by MCR supplemented. Microbial metabolic phenotypes analysis also showed that the richness of aerobic bacteria and facultative anaerobic bacteria, oxidative stress tolerance, and biofilm forming were significantly increased (P < 0.05), and the richness of anaerobic bacteria and pathogenic potential of gut microbiota were reduced (P < 0.05) by MCR treatment. Conclusions: In antibiotic-free diets, MCR supplementation improved growth performance and serum antioxidant capacity, alleviated intestinal inflammatory by inhibiting IKKβ/IκBα/NF-κB signaling pathway and affecting intestinal microbiota in weaned piglets.

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Section: Discussionsupporting

confidence: 66%

Dietary Moutan Cortex Radicis Alters Serum Antioxidant Capacity, Intestinal Immunity and Colonic Microbiota in Weaned Piglets

Bai

Liu

Wang

et al. 2020

Preprint

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“…Enteroviruses, such as poliovirus, coxsackievirus, and echovirus, reproduce in the intestine and can invade intestinal epithelial cells [48]. The inflammatory response to host cell death can decrease the expression of the intestinal barrier proteins ZO-1, occludin or claudin 3, which disrupts the integrity of the intestinal barrier [49,50]. Besides, the inflammatory response can also trigger intestinal microbiota disorder, which aggregates the damage of the intestinal mucosa barrier structure [51].…”

Section: Digestive Tract Routementioning

confidence: 99%

Neurological manifestations of patients with COVID-19: potential routes of SARS-CoV-2 neuroinvasion from the periphery to the brain

et al. 2020

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Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused a global pandemic in only 3 months. In addition to major respiratory distress, characteristic neurological manifestations are also described, indicating that SARS-CoV-2 may be an underestimated opportunistic pathogen of the brain. Based on previous studies of neuroinvasive human respiratory coronaviruses, it is proposed that after physical contact with the nasal mucosa, laryngopharynx, trachea, lower respiratory tract, alveoli epithelium, or gastrointestinal mucosa, SARS-CoV-2 can induce intrinsic and innate immune responses in the host involving increased cytokine release, tissue damage, and high neurosusceptibility to COVID-19, especially in the hypoxic conditions caused by lung injury. In some immunecompromised individuals, the virus may invade the brain through multiple routes, such as the vasculature and peripheral nerves. Therefore, in addition to drug treatments, such as pharmaceuticals and traditional Chinese medicine, non-pharmaceutical precautions, including facemasks and hand hygiene, are critically important.

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“…53 Li C et al found that upregulating ZO-1/occludin/claudin-1 expression mitigates the in ammatory response and maintains intestinal barrier function in sepsis, providing a good experimental basis for its clinical treatment of sepsis. 54 And Lee TJ et al found that ZO-1 on the exotoxin LPS of P. aeruginosa-induced diseases could be critical in the development of novel therapeutics. 55 It is interesting that, Na, K-ATPase β1 promotes the expression of key proteins such as ZO-1, ZO-2, occludin and claudin-18 in tight junction complex and reduces the production of lung water.…”

Section: Discussionmentioning

confidence: 99%

Identification of Different Proteins Binding to Na, K-ATPase α1 in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome Cell Model by Proteomic Analysis

Wen

Zhang

Huang

et al. 2021

Preprint

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Acute respiratory distress syndrome (ARDS) is characterized by refractory hypoxemia caused by accumulation of pulmonary fluid, which is related to inflammatory cell infiltration, impaired tight junction of pulmonary epithelium and impaired Na, K-ATPase function, especially Na, K-ATPase α1 subunit. Up until now, the pathogenic mechanism at the level of protein during lipopolysaccharide- (LPS-) induced ARDS remains unclear. Using an unbiased, discovery and quantitative proteomic approach, the discovery of differentially expressed proteins binding to Na, K-ATPase α1 between LPS-induced A549 cell and control-A549 group is of particular interest for the current study. These proteins may help the clinical diagnosis and facilitate the personalized treatment of ARDS. We screened these Na, K-ATPase α1 interacting proteins, carried out the related Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, and found evident phenomena of ubiquitination and deubiquitination, as well as the pathways related to autophagy. We also chose some of the differentiated expressing proteins with significant performance for further verification by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among proteins with rich abundance, there were several intriguing ones, including the deubiquitinase (OTUB1), the tight junction protein zonula occludens-1 (ZO-1), the scaffold protein in CUL4B-RING ubiquitin ligase (CRL4B) complexes (CUL4B) and the autophagy-related protein sequestosome-1 (SQSTM1). Protein-protein interaction network showed that there were 244 significantly enriched co-expression among 60 proteins in the group control-A549. while the group LPS-A549 showed 43 significant enriched interactions among 29 proteins. In conclusion, our quantitative discovery-based proteomic approach identified commonalities, and revealed targets related to the occurrence and development of ARDS, being the first study to investigate significant differences in Na, K-ATPase α1 interacting proteins between LPS-induced ARDS cell model and control-A549 cell.

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Tong-fu-li-fei decoction exerts a protective effect on intestinal barrier of sepsis in rats through upregulating ZO-1/occludin/claudin-1 expression

Cited by 37 publications

References 9 publications

Dietary Moutan Cortex Radicis Alters Serum Antioxidant Capacity, Intestinal Immunity and Colonic Microbiota in Weaned Piglets

Dietary Moutan Cortex Radicis Alters Serum Antioxidant Capacity, Intestinal Immunity and Colonic Microbiota in Weaned Piglets

Neurological manifestations of patients with COVID-19: potential routes of SARS-CoV-2 neuroinvasion from the periphery to the brain

Identification of Different Proteins Binding to Na, K-ATPase α1 in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome Cell Model by Proteomic Analysis

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