Tombusviridae

Sit, Tim L.; Lommel, Steven A.

doi:10.1002/9780470015902.a0000756.pub3

Cited by 5 publications

(4 citation statements)

References 57 publications

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“…We identified a high diversity and abundance of novel Tombusviridae, a family of single-strand positive-sense RNA viruses that infect plants (Sit and Lommel 2015). Of the viruses identified 18 fell into the subfamily Procedovirinae , found in all land-use types.…”

Section: Resultsmentioning

confidence: 99%

Human land-use impacts viral diversity and abundance in a New Zealand river

French

Charon

Lay

et al. 2022

Preprint

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Although water borne viruses have important implications for the health of humans and other animals, little is known about the impact of human land use on viral diversity and evolution in water systems such as rivers. We used metagenomic next generation sequencing to compare the diversity and abundance of viruses at sampling sites along a single river in New Zealand that differed in human land use impact, ranging from pristine to urban. From this we identified 504 putative virus species, of which 97% were novel. Many of the novel viruses were highly divergent, and likely included a new subfamily within the Parvoviridae. We identified at least 63 virus species that may infect vertebrates, most likely fish and water birds, from the Astroviridae, Birnaviridae, Parvoviridae and Picornaviridae. No putative human viruses were detected. Importantly, we observed differences in the composition of viral communities at sites impacted by human land use (farming and urban) compared to native forest sites (pristine). At the viral species level, the urban sites had higher diversity (327 virus species) than the farming (n=150) and pristine sites (n=119), and more viruses were shared between the urban and farming sites (n=76) than between the pristine and farming or urban sites (n=24). The two farming sites had a lower viral abundance across all host types, while the pristine sites had a higher abundance of viruses associated with animals, plants and fungi. We also identified viruses linked to agriculture and human impact at the river sampling sites in farming and urban areas that were not present at the native forest sites. Overall, our study shows that human land use can impact viral communities in rivers, such that further work is needed to reduce the impact of intensive farming and urbanization on water systems.

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Section: Resultsmentioning

confidence: 99%

Human land-use impacts viral diversity and abundance in a New Zealand river

French

Charon

Lay

et al. 2022

Preprint

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“…As stated previously (Pérez-Cañamás and Hernández 2015), the NoLS-containing region of p37 is also involved in other relevant properties of the protein such as the capacity to bind small RNAs, which is essential for its VSR function. In addition, it forms very likely part of the so-called RNA binding domain that has been proposed to directly interact with viral ssRNA for virion formation in related CPs (Sit and Lommel 2015;Rao et al 2006). These observations emphasize once more the high overlap of motifs involved in different functional traits of p37, as we stressed in a previous study (Pérez-Cañamás and Hernández 2015).…”

Section: Discussionmentioning

confidence: 99%

New Insights into the Nucleolar Localization of a Plant RNA Virus-Encoded Protein That Acts in Both RNA Packaging and RNA Silencing Suppression: Involvement of Importins Alpha and Relevance for Viral Infection

Pérez-Cañamás

Hernández

2018

MPMI

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Despite the fact that replication of plus-strand RNA viruses takes place in the cytoplasm of host cells, different proteins encoded by these infectious agents have been shown to localize in the nucleus, with high accumulation at the nucleolus. In most cases, the molecular determinants or biological significance of such subcellular localization remains elusive. Recently, we reported that protein p37 encoded by Pelargonium line pattern virus (family Tombusviridae) acts in both RNA packaging and RNA silencing suppression. Consistently with these functions, p37 was detected in the cytoplasm of plant cells, although it was also present in the nucleus and, particularly, in the nucleolus. Here, we searched for further insights into factors influencing p37 nucleolar localization and into its potential relevance for viral infection. Besides mapping the protein region containing the nucleolar localization signal, we have found that p37 interacts with distinct members of the importin alpha family-main cellular transporters for nucleo-cytoplasmic traffic of proteins-and that these interactions are crucial for nucleolar targeting of p37. Impairment of p37 nucleolar localization through downregulation of importin alpha expression resulted in a reduction of viral accumulation, suggesting that sorting of the protein to the major subnuclear compartment is advantageous for the infection process.

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“…Tombusviridae is a large plant virus family whose members contain (+) RNA genomes [45]. All members of this family are non-segmented, except for those in the genus Dianthovirus .…”

Section: Dianthovirusesmentioning

confidence: 99%

Trans-Acting RNA–RNA Interactions in Segmented RNA Viruses

Newburn

White

2019

Viruses

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RNA viruses represent a large and important group of pathogens that infect a broad range of hosts. Segmented RNA viruses are a subclass of this group that encode their genomes in two or more molecules and package all of their RNA segments in a single virus particle. These divided genomes come in different forms, including double-stranded RNA, coding-sense single-stranded RNA, and noncoding single-stranded RNA. Genera that possess these genome types include, respectively, Orbivirus (e.g., Bluetongue virus), Dianthovirus (e.g., Red clover necrotic mosaic virus) and Alphainfluenzavirus (e.g., Influenza A virus). Despite their distinct genomic features and diverse host ranges (i.e., animals, plants, and humans, respectively) each of these viruses uses trans-acting RNA–RNA interactions (tRRIs) to facilitate co-packaging of their segmented genome. The tRRIs occur between different viral genome segments and direct the selective packaging of a complete genome complement. Here we explore the current state of understanding of tRRI-mediated co-packaging in the abovementioned viruses and examine other known and potential functions for this class of RNA–RNA interaction.

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Tombusviridae

Cited by 5 publications

References 57 publications

Human land-use impacts viral diversity and abundance in a New Zealand river

Human land-use impacts viral diversity and abundance in a New Zealand river

New Insights into the Nucleolar Localization of a Plant RNA Virus-Encoded Protein That Acts in Both RNA Packaging and RNA Silencing Suppression: Involvement of Importins Alpha and Relevance for Viral Infection

Trans-Acting RNA–RNA Interactions in Segmented RNA Viruses

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