TOL plasmid carriage enhances biofilm formation and increases extracellular DNA content in Pseudomonas putida KT2440

D’Alvise, Paul; Sjøholm, Ole Rüdiger; Yankelevich, Tatiana; Jin, Yujie; Wuertz, Stefan; Smets, Barth F.

doi:10.1111/j.1574-6968.2010.02105.x

Cited by 38 publications

(30 citation statements)

References 47 publications

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“…Many of the aforementioned examples of biofilm‐enabling plasmids originate from E. coli , but biofilm priming encoded on plasmids has been identified in both Gram‐negative and Gram‐positive bacteria such as the Pseudomonas putida TOL plasmid (D'Alvise et al ., ), Lactococcus lactis pAMβ1 (Lou et al ., ), Azospirillum brasilense plasmids (Pentrova et al ., ) and the Enterococcus faecialis pBEE99. Similar to E. coli , E. faecialis commonly occupy a commensal niche in the gastrointestinal tract but also appear as opportunistic pathogens.…”

Section: The Role Of Plasmids In Biofilm Formation and Stabilizationmentioning

confidence: 99%

The interconnection between biofilm formation and horizontal gene transfer

Madsen

Burmølle

Hansen

et al. 2012

FEMS Immunol Med Microbiol

523

377

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Recent research has revealed that horizontal gene transfer and biofilm formation are connected processes. Although published research investigating this interconnectedness is still limited, we will review this subject in order to highlight the potential of these observations because of their believed importance in the understanding of the adaptation and subsequent evolution of social traits in bacteria. Here, we discuss current evidence for such interconnectedness centred on plasmids. Horizontal transfer rates are typically higher in biofilm communities compared with those in planktonic states. Biofilms, furthermore, promote plasmid stability and may enhance the host range of mobile genetic elements that are transferred horizontally. Plasmids, on the other hand, are very well suited to promote the evolution of social traits such as biofilm formation. This, essentially, transpires because plasmids are independent replicons that enhance their own success by promoting inter‐bacterial interactions. They typically also carry genes that heighten their hosts’ direct fitness. Furthermore, current research shows that the so‐called mafia traits encoded on mobile genetic elements can enforce bacteria to maintain stable social interactions. It also indicates that horizontal gene transfer ultimately enhances the relatedness of bacteria carrying the mobile genetic elements of the same origin. The perspective of this review extends to an overall interconnectedness between horizontal gene transfer, mobile genetic elements and social evolution of bacteria.

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Section: The Role Of Plasmids In Biofilm Formation and Stabilizationmentioning

confidence: 99%

The interconnection between biofilm formation and horizontal gene transfer

Madsen

Burmølle

Hansen

et al. 2012

FEMS Immunol Med Microbiol

523

377

View full text Add to dashboard Cite

show abstract

“…In addition, a structural function has been suggested, for instance for Staphylococcus aureus where it is linking beta toxin in the presence of eDNA, resulting in skeletal framework as basis for biofilm formation [61]. For P. putida the presence of the TOL-plasmid (responsible for toluene and xylene mineralization) increases the amount of eDNA and results in the formation of pellicles and thick biofilms [62]. These effects are likely to be mediated by the physical properties of eDNA, which increases the cell surface hydrophobicity and this also increases adhesion to hydrophobic surfaces for Streptococcus mutans [63].…”

Section: Extracellular Dnamentioning

confidence: 99%

EPS—Then and Now

2016

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“Slime” played a brief and spectacular role in the 19th century founded by the theory of primordial slime by Ernst Haeckel. However, that substance was never found and eventually abandoned. Further scientific attention slowly began in the 1930s referring to slime as a microbial product and then was inspired by “How bacteria stick” by Costerton et al. in 1978, and the matrix material was considered to be polysaccharides. Later, it turned out that proteins, nucleic acids and lipids were major other constituents of the extracellular polymeric substances (EPS), an acronym which was highly discussed. The role of the EPS matrix turns out to be fundamental for biofilms, in terms of keeping cells in proximity and allowing for extended interaction, resource capture, mechanical strength and other properties, which emerge from the life of biofilm organisms, including enhanced tolerance to antimicrobials and other stress. The EPS components are extremely complex and dynamic and fulfil many functional roles, turning biofilms into the most ubiquitous and successful form of life on Earth.

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“…Such structured bacterial communities attached to a surface or to each other are called biofilms (16). The presence of transferrable plasmids and conjugation events has been shown to positively affect biofilm formation (10,21,29,50,54,57,69). Since bacterial cells in biofilms stay in close contact, it is believed that gene exchange by conjugation is favored by this "biofilm mode" of growth.…”

mentioning

confidence: 99%

Increased Transfer of a Multidrug Resistance Plasmid in Escherichia coli Biofilms at the Air-Liquid Interface

Król

Nguyen

Rogers

et al. 2011

Appl Environ Microbiol

102

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Although biofilms represent a common bacterial lifestyle in clinically and environmentally important habitats, there is scant information on the extent of gene transfer in these spatially structured populations. The objective of this study was to gain insight into factors that affect transfer of the promiscuous multidrug resistance plasmid pB10 in Escherichia coli biofilms. Biofilms were grown in different experimental settings, and plasmid transfer was monitored using laser scanning confocal microscopy and plate counting. In closed flow cells, plasmid transfer in surface-attached submerged biofilms was negligible. In contrast, a high plasmid transfer efficiency was observed in a biofilm floating at the air-liquid interface in an open flow cell with low flow rates. A vertical flow cell and a batch culture biofilm reactor were then used to detect plasmid transfer at different depths away from the air-liquid interface. Extensive plasmid transfer occurred only in a narrow zone near that interface. The much lower transfer frequencies in the lower zones coincided with rapidly decreasing oxygen concentrations. However, when an E. coli csrA mutant was used as the recipient, a thick biofilm was obtained at all depths, and plasmid transfer occurred at similar frequencies throughout. These results and data from separate aerobic and anaerobic matings suggest that oxygen can affect IncP-1 plasmid transfer efficiency, not only directly but also indirectly, through influencing population densities and therefore colocalization of donors and recipients. In conclusion, the air-liquid interface can be a hot spot for plasmid-mediated gene transfer due to high densities of juxtaposed donor and recipient cells.

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TOL plasmid carriage enhances biofilm formation and increases extracellular DNA content in Pseudomonas putida KT2440

Cited by 38 publications

References 47 publications

The interconnection between biofilm formation and horizontal gene transfer

The interconnection between biofilm formation and horizontal gene transfer

EPS—Then and Now

Increased Transfer of a Multidrug Resistance Plasmid in Escherichia coli Biofilms at the Air-Liquid Interface

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