Tobacco Dust induced genotoxicity as an occupational hazard in workers of bidi making cottage industry of central India

Abstract

Context:To explore genotoxicity in bidi rollers occupationally exposed to bidi tobacco dust.Aims:To assess the extent of genotoxicity of tobacco dust to bidi rollers of Jabalpur, Madhya Pradesh, India and cytotoxicity of bidi tobacco extract.Settings and Design:Blood samples from 31 bidi rollers and 30 controls taken after written informed consent were analyzed for chromosome aberrations (CA) and comet assay.Materials and Methods:Genotoxicity was studied by CA in cultured peripheral blood lymphocytes of bidi rollers and the deoxyribonucleic acid (DNA) damage studies were done by comet assay of their blood. The toxicity of bidi tobacco extract to normal human lymphocytes was studied by MMT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay as drop in viability.Statistical Analysis Used:Student's t-test and DMRT.Results:There is a general trend of increase in CA% of both in exposed and control groups with age, but in every group the bidi rollers have a significantly higher CA% than the controls. The CA % is also directly related to exposure. The comet assay findings reveal that the mean comet length and tail length increases with exposure time. The toxicity of bidi tobacco extract (TE) to normal human lymphocytes was tested in vitro by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay at 2 h of incubation. The trend of drop in viability with increasing concentrations of TE was clearly evident from the data from four donors in spite of their individual differences in viability.Conclusions:The results obtained in this investigation indicate that bidi rollers seem to be facing the occupational hazard of genotoxicity due to handling bidi tobacco and inhalation of tobacco dust. They should be advised to work under well-ventilated conditions.

Toxicity of Imidacloprid on Peripheral Blood Lymphocytes by MTT Assay and the Ameliorative Effect of Extract of Tinospora cordifolia (Gilloe) Extract

“…Separation of lymphocyte from blood was done by standard method explained elsewhere (15). A preinformed, willing female donor in good health was requested to donate 3 ml of the blood for the investigation.…”

Tobacco dust, genotoxicity, and bidi-making cottage industry

“…[ 2 ]” Mahimkar and Bhisey reported for “a significant increase in deletion fragments and chromatid gaps in the exposed group. [ 2 ]” However, based on the present report by Khanna et al .,[ 1 ] it can confirm only that there is genotoxicity; however, the relationship to exposure might not be concluded. In fact, to confirm the exposure, biomarker monitoring is required.…”

“…[1] Khanna et al . noted that “bidi rollers seem to be facing the occupational hazard of genotoxicity due to handling bidi tobacco and inhalation of tobacco dust.…”

“…The report on “tobacco Dust induced genotoxicity as an occupational hazard in workers of bidi making cottage industry of central India” is very interesting. [ 1 ] Khanna et al . noted that “bidi rollers seem to be facing the occupational hazard of genotoxicity due to handling bidi tobacco and inhalation of tobacco dust.…”

“…noted that “bidi rollers seem to be facing the occupational hazard of genotoxicity due to handling bidi tobacco and inhalation of tobacco dust. [ 1 ]” Indeed, this topic is not a new thing. Previously, Mahimkar and Bhisey reported that “occupational exposure to bidi tobacco increases chromosomal aberrations in tobacco processors.…”