To Study the Usefulness of Cbnaat (Cartridge Based Nuclear Acid Amplification Test) in Bal (Bronchoalveolar Lavage) Samples in the Diagnosis of Smear-Negative/Non Sputum Producing Patients With Suspected Tuberculosis

Avashia, Sanjay; Choubey, Satyadeo; Kharate, Atul

doi:10.14260/jemds/2016/13

Cited by 10 publications

(10 citation statements)

References 10 publications

(10 reference statements)

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“…Most common symptom in our study was cough (66%%) followed by loss of appetite (69.4 %) and fever (54.4%). Cough was also chief presenting symptom in study done by Pierre Le Palud et al (51.9%) [11] Vivek N. Iyer et al [10] 60%, Poojan shrestha et al [13] 71.7%, Sanjay awashiya et al [9] 72.2%.…”

Section: Discussionmentioning

confidence: 94%

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To Study the Usefulness of Cartridge Based Nuclear Acid Amplification Test [CBNAAT] in Bronchoalveolar Samples in the Diagnosis of Sputum Negative Patients with Presumptive Pulmonary Tuberculosis

Dash

2019

IJOPRS

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Background: About 40-60% of patients with presumptive PTB may fail to produce sputum, or when it is available, AFB/CBNAAT may be negative on repeated smear examination. These patients can be diagnosed by flexible fibreoptic bronchoscopy.Aims: This study was carried out to know the usefulness of bronchoscopy in sputum negative suspected pulmonary tuberculosis patients. Material and method:We identified 68 consecutive cases of suspected pulmonary TB between Nov 2016 and July 2017, who had negative results on sputum smears examination by fluorescent microscopy/ZN staining on at least 2 samples or CBNAAT and did fibre optic bronchoscopy and sent bronchial aspirate for CBNAAT and AFB staining. Result:Males constituted majority of our study population. The most common age group involved in the study was less than 30 years (35.2%). Cough was the most common symptom reported by 45 patients (66.1%). The past history of PTB was present 13 patients (19.1%). 27.9% of study population had consolidation on CXR. Out of 68 clinically suspected sputum negative-PTB patients, 32 patients (47%) were finally diagnosed as having microbiologically confirmed PTB. Conclusion:CBNAAT done on bronchial aspirate can be an important adjunct to bacteriological confirmation of suspected cases who were otherwise sputum negative or not expectorating adequate sputum.

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Section: Discussionmentioning

confidence: 94%

“…Although the relative transmission rate of smear negative tuberculosis is lower than that of smear positive cases, it is still responsible for 17% of tuberculosis transmission [9]. The diagnosis of pulmonary TB in patients with negative expectorated sputum results remains challenging due to variable diagnostic yields of any single test [10].…”

Section: Discussionmentioning

confidence: 99%

To Study the Usefulness of Cartridge Based Nuclear Acid Amplification Test [CBNAAT] in Bronchoalveolar Samples in the Diagnosis of Sputum Negative Patients with Presumptive Pulmonary Tuberculosis

Dash

2019

IJOPRS

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“…8 Others were pus, lymph node and synovial fluid. Out of these 37% (111) patients were Gene Xpert MTB/RIF Assay positive and of these only 36% (40 out of 111) were ZN smear positive.…”

Section: Discussionmentioning

confidence: 99%

Comparison of diagnostic yield of GeneXpert MTB/RIF assay and ZN (Ziehl-Neelsen) staining in serosal fluids from HIV and non-HIV patients with extra-pulmonary tuberculosis

Mittal

Kumar

2017

Int J Res Med Sci

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Background: India accounts for 23% of the global TB burden and of these 15-20% are extrapulmonary tuberculosis(EPTB). EPTB has diverse clinical presentation and often is over diagnosed because of lack of standardised diagnostic means. Yield of acid fast bacilli (AFB) from EPTB settings is very low and alternate facilities are nearly non-existent routinely. GeneXpert MTB/RIF has been recommended as a diagnostic tool for sputum samples. It has been also recommended for serosal fluids from HIV patients only. This study was done to compare the yield of GeneXpert MTB/RIF assay and ZN (Ziehl Neelsen) staining among HIV and non-HIV patients with suspected serosal TB.Methods: GeneXpert MTB/RIF assay and ZN staining was done in all serosal fluid samples in which a clinical diagnosis of serosal TB had been made by conventional methods.Results: A total of 81 extra pulmonary samples (21 from HIV and 60 from non-HIV patients) were processed in this study, which included 48 CSF, 19 pleural fluids and 14 ascitic fluid. Out of these, 34.5% (28/81) patients were both GeneXpert MTB/RIF as well as ZN stain positive whereas only 13.58%(11/81) patients were ZN stain positive. 4/21 (19.05%) samples from HIV patients were ZN stain positive and 9/21(42.85%) were GeneXpert positive. 7/60 (11.66%) samples from non-HIV patients were ZN stain positive and 19/60 (31.66%) were GeneXpert positive.Conclusions: GeneXpert MTB/RIF is more sensitive than ZN staining in EPTB. The yield in both HIV and non-HIV patients is the same.

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“…Exclusion Criteria: Patients with extrapulmonary tuberculosis based on clinical findings and comorbidities with chronic inflammatory diseases 12 . Samples received without clinical history or received without consent and requisition form.…”

Section: Methodsmentioning

confidence: 99%

“…Smear Microscopy: For microscopy examination, an oval or round-shaped direct smear was fixed on a clean grease-free slide and stained by using Ziehl-Neelsen Stain (ZN-Stain). Now, stained smear focused under oil immersion objective (100x) 12 . The minimum number of acid-fast bacilli present in a smear after observing a minimum 100 fields was an indication of the severity of pulmonary tuberculosis infection of the patient.…”

Section: Specimen Collection Storage and Transportationmentioning

confidence: 99%

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2020

IJPSR

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Background: Tuberculosis (TB) is a global health burden and can cause potential infection in any system of the body. Pulmonary tuberculosis (PTB) is most common presentation, but the diagnosis of TB remains elusive because of none of biochemical or serological test is invalid. During the development of END TB strategy, the gene expert (CBNAAT) test was first endorsed by WHO. Materials and Methods: This was a prospective observational study; conducted in the

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To Study the Usefulness of Cbnaat (Cartridge Based Nuclear Acid Amplification Test) in Bal (Bronchoalveolar Lavage) Samples in the Diagnosis of Smear-Negative/Non Sputum Producing Patients With Suspected Tuberculosis

Cited by 10 publications

References 10 publications

To Study the Usefulness of Cartridge Based Nuclear Acid Amplification Test [CBNAAT] in Bronchoalveolar Samples in the Diagnosis of Sputum Negative Patients with Presumptive Pulmonary Tuberculosis

To Study the Usefulness of Cartridge Based Nuclear Acid Amplification Test [CBNAAT] in Bronchoalveolar Samples in the Diagnosis of Sputum Negative Patients with Presumptive Pulmonary Tuberculosis

Comparison of diagnostic yield of GeneXpert MTB/RIF assay and ZN (Ziehl-Neelsen) staining in serosal fluids from HIV and non-HIV patients with extra-pulmonary tuberculosis

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