1991
DOI: 10.1128/jb.173.5.1617-1622.1991
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Abstract: that the conjugative transfer of all these elements shares a common mechanism, as the elements are derived from an ancestral element with transfer properties.Tn5253, formerly called the fl(cat tet) element (36), was originally detected as a heterologous insertion in the chromosome of the plasmid-free clinical isolate S. pneumoniae BM6001 (3, 9). By inserting the Escherichia coli vector plasmid pVA891 (22) (which is incapable of autonomous replication in streptococci) at many sites specifically within Tn5253, w… Show more

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Cited by 127 publications
(106 citation statements)
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“…De igual manera, la ausencia de la banda se asoció con aislamientos susceptibles a estos dos antibióticos (5). Este fragmento de 340 Kb contiene los genes tetM y cat (2), que confieren resistencia a tetraciclina y cloranfenicol respectivamente, y que hacen parte del transposón conjugativo Tn5253 (19). La presencia de la banda puede explicarse por la inserción de este transposón en el genoma del neumococo, evento genético que puede alterar los sitios de restricción de la enzima Sma I y generar nuevos patrones electroforéticos (13,20).…”
Section: Resultsunclassified
“…De igual manera, la ausencia de la banda se asoció con aislamientos susceptibles a estos dos antibióticos (5). Este fragmento de 340 Kb contiene los genes tetM y cat (2), que confieren resistencia a tetraciclina y cloranfenicol respectivamente, y que hacen parte del transposón conjugativo Tn5253 (19). La presencia de la banda puede explicarse por la inserción de este transposón en el genoma del neumococo, evento genético que puede alterar los sitios de restricción de la enzima Sma I y generar nuevos patrones electroforéticos (13,20).…”
Section: Resultsunclassified
“…Cat causes enzymatic modification, which is associated with chloramphenicol acetylation and the generation of inoperative O-acetoxy chloramphenicol products, further hampering its successful binding to ribosomes [84,85]. In S. pneumoniae, the cat gene is carried by Tn5253, a composite transposon identified in 1991 [74]. In 2000, a linearized version of pC194, a cat carrier plasmid originally described in Staphylococcus aureus, was found integrated into the S. pneumoniae chromosome [86].…”
Section: Chloramphenicol Resistancementioning
confidence: 99%
“…This genetic element was first named omega cat-tet or (cat-tet) [73], and was then renamed Tn5253 [74]. Tn5253 is composed of two distinct conjugative transposons, each displaying its own conjugal proprieties: (1) Tn5251 (related to the Tn916 Ala203Val murM Mosaic gene Penicillin and cephalosporin [23] family), the 18 kb tet-carrier transposon inserted within (2) Tn5252, the cat-carrier that comprises the rest of Tn5253 [74]. Moreover, Tn5251 has shown the potential for independent transfer to a variety of bacterial species after excision from Tn5253 and a capacity to combine with other resistance-determinant carriers [75].…”
Section: Tetracycline Resistancementioning
confidence: 99%
“…Due to their ability to integrate into DNA, it should not be surprising to find chimeric transposons, i.e., a transposon integrated into another transposon. However, to date, there are only two chimeric transposons in which a CTn inserted into another CTn, have been reported, a CTn in grampositive bacteria, the streptococcal Tn5253 76 ; and a CTn in gram negative bacteria, the Bacteroides CTn12256. 77 Although the 188kb CTn12256 was isolated from a clinical isolate in 1977, only recently has its chimeric nature been studied in more detail.…”
Section: Mobile Genetic Elements In the Bacteroidesmentioning
confidence: 99%