Títulos de anticorpos aglutinantes induzidos por vacinas comerciais contra leptospirose bovina

Arduino, Gabriela de Godoy Cravo; Gírio, Raul José Silva; Magajevski, Fernanda Senter; Pereira, Gener Tadeu

doi:10.1590/s0100-736x2009000700013

Cited by 13 publications

(7 citation statements)

References 8 publications

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“…As vacinas anti-leptospira produzidas com antígeno inativado são imunógenos fracos, gerando resposta imune apenas do tipo humoral, em muitas ocasiões com baixo tí-tulo de anticorpos e que persistem por um curto período de tempo (Bolin et al 1989, Arduino et al 2009). Em estudo recente avaliou-se bacterinas polivalentes produzidas comercialmente para bovinos e uma vacina monovalente produzida com a sorovariedade Hardjo.…”

Section: Discussionunclassified

Controle da leptospirose em bovinos de leite com vacina autógena em Santo Antônio do Monte, Minas Gerais

et al. 2012

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Um surto de leptospirose foi observado em bovinos leiteiros em Santo Antônio do Monte, Minas Gerais. O rebanho apresentava reações positivas anti-leptospira sorovar Hardjo no teste de microaglutinação (MAT) e havia sido vacinado anteriormente com vacina experimental contendo a sorovariedade Hardjo. O MAT revelou 48,06% dos bovinos positivos para sorovariedade Hardjo genótipo Hardjobovis, 36,82% para sorovariedade Hardjo genótipo Hardjoprajitno. Os animais apresentavam aborto e mastite com presença de sangue no leite. A presente pesquisa teve como objetivos isolar as sorovariedades existentes a partir da urina de vacas sorologicamente positivas, elaborar uma vacina experimental com as sorovariedades isoladas no rebanho, avaliar a eficiência do programa de vacinação por um período de dois anos por meio da sorologia do rebanho. Foi isolada Leptospira spp. a partir da urina de duas vacas com sinais sugestivos da doença. As amostras isoladas foram identificadas pela sorologia com anticorpos monoclonais e seqüenciamento do gene 16S rRNA como pertencentes à espécie Leptospira interrogans, sorogrupo Sejroe, sorovariedade Hardjo e genótipo Hardjoprajitno. O uso da vacina autógena foi eficaz no controle da leptospirose no rebanho no período de dois anos. Os resultados da sorologia revelaram ausência de animais positivos na última prova realizada no rebanho.

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Section: Discussionunclassified

Controle da leptospirose em bovinos de leite com vacina autógena em Santo Antônio do Monte, Minas Gerais

et al. 2012

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“…For this reason, it is important to apply control measures to avoid the introduction of animals in the herd without quarantine, avoid the presence of wild animals that can serve as disease reservoirs, avoid the slaughter of animals on the property, avoid the use of shared pastures, and immunize the herd using vaccines containing the serovars that are most common in the territory (OLIVEIRA et al, 2010;ARDUINO et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Seroepidemiology of leptospirosis in dairy cattle in Ipameri, state of Goiás, Brazil

Paim

Ciuffa

Gomes

et al. 2016

SCA

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Leptospirosis is a disease with worldwide distribution and with risk to human health. In addition, it affects farm animals, and consequently, causes economic losses to farmers. Therefore, understanding the epidemiological profile of the disease in each geographical region is essential. This study was conducted in the rural properties of the municipality of Ipameri, state of Goiás, Brazil, where leptospirosis has not been reported in dairy farming. The objective of this study was to determine the seroprevalence of leptospirosis in dairy cattle, identify the serovars present, and correlate these results with factors that could favor the appearance of the disease. For this purpose, 285 blood samples were collected from dairy cows and microscopic agglutination tests were conducted to identify the 15 serovars of Leptospira interrogans. A structured questionnaire was applied during the visits to the properties to assess the factors associated with leptospirosis. The prevalence was found to be 18.9%, and the serovars present in the region included Hardjo, Wolffi, Canicola, Hebdomadis, Australis, and Icterohaemorrhagiae. The variables associated with leptospirosis were the presence of reproductive changes in the herd, presence of rodents, increased daily production of milk, hired labor, and lower level of education of the producers. Key words: Leptospira. Reproduction. Microscopic agglutination test. Production systems. ResumoA leptospirose é uma doença de distribuição mundial, que oferece riscos à saúde humana e acomete os animais de produção, gerando perdas econômicas ao produtor rural, sendo importante conhecer o perfil epidemiológico da doença em cada região. Esse estudo foi realizado em propriedades rurais do município de Ipameri, estado de Goiás, onde não existia nenhum relato sobre a leptospirose na pecuária leiteira. O objetivo desta pesquisa foi determinar a soroprevalência de leptospirose nos bovinos leiteiros, os soravares presentes e correlacionar os resultados com variáveis que podem favorecer o aparecimento da doença. Para isso foram coletadas 285 amostras de sangue de vacas em lactação e realizado o teste diagnóstico de soroaglutinação microscópica em campo escuro (SAM) contra 15 sorovares de Leptospira interrogans. Aplicou-se um questionário estruturado durante a visita para avaliar os fatores associados à ocorrência da leptospirose. A prevalência encontrada foi de 18,9%, e os

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“…For sera screening, a 1:100 dilution was used, and the samples were considered reactive when they showed at least 50% of agglutination on the microscope field. This dilution was used as a cutoff point, as stated by Nardi Júnior et al (2006) and Arduino et al (2009). The reactive sera were titrated with increasing dilutions (1:200, 1:400, 1:800, 1:1600, 1:3200).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of response to anti-leptospira bacterin vaccination in pregnant ewes and the passive transfer of antibodies to their offspring

et al. 2020

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Leptospirosis in sheep is often underestimated, and leads to great economic losses for the sheep farming industry. The aim of this study was to evaluate the humoral immune response in pregnant ewes, after the injection of a commercial polyvalent vaccine for leptospirosis, and to observe the transmission of anti-Leptospira antibodies through the colostrum to the offspring. For this, 24 pregnant ewes were vaccinated for leptospirosis. Blood samples were collected prior to vaccination and then 7, 14, 21, 28, 35, 42 and 49 days after vaccination. In order to evaluate passive immunity transfer, blood samples of 32 lambs were collected during the first 48 hours after birth, and another collection was performed 10 to 21 days after birth. The lambs were placed into 2 groups: Group A (n=16): singleton lambs; and group B (n=16): twins. The sera samples were submitted to the Microscopic Agglutination Test (MAT), in which 21 Leptospira serovars were tested. The results were analyzed in a descriptive form. The number of sheep reactive to MAT gradually increased until 21 days after vaccination, and decreased right after. Of all the serovars contained in the vaccine, the largest proportion of animals were seroconverted to Hardjoprajtino serovar, Serjoe serogroup. Anti-Leptospira antibodies transferred through colostrum to lambs were detected by MAT in the serum collected 24-48 hours after birth. It was observed that 65.6% (21 out of 32) of the lambs were reactive. In the subsequent collections that occurred from 10 to 21 days after birth, a decrease in the number of animals reactive to the MAT was detected. There was no significant statistical difference for the passive transfer of antibodies between single or twin lambs.

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Títulos de anticorpos aglutinantes induzidos por vacinas comerciais contra leptospirose bovina

Cited by 13 publications

References 8 publications

Controle da leptospirose em bovinos de leite com vacina autógena em Santo Antônio do Monte, Minas Gerais

Controle da leptospirose em bovinos de leite com vacina autógena em Santo Antônio do Monte, Minas Gerais

Seroepidemiology of leptospirosis in dairy cattle in Ipameri, state of Goiás, Brazil

Evaluation of response to anti-leptospira bacterin vaccination in pregnant ewes and the passive transfer of antibodies to their offspring

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