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Matthews, R.E.F.

doi:10.1159/000149278

Cited by 581 publications

(51 citation statements)

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“…distinguish it from all other known viruses. Structurally, the virion is very large with a symmetry that is unique and is best characterized as being of the complex type (9). The shape of the virion, which is determined by the inner particle, is essentially allantoid, although in thin sections the inner particle or mature virion can appear oval, reniform, elliptical, or naviculate, depending on the plane of section through the inner particle or enveloped virion.…”

Section: Resultsmentioning

confidence: 99%

Enveloped double-stranded DNA insect virus with novel structure and cytopathology

Federici

1983

Proc. Natl. Acad. Sci. U.S.A.

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An unusual type of virus has been isolated from larvae of the cabbage looper, Trichoplusia ni (Lepidoptera; Noctuidae). The virus infects a variety of tissues, including fat body, epidermis, and tracheal matrix, causing a chronic, fatal disease. Viral replication begins in the nucleus and is accompanied by invagination of the nuclear envelope and extensive nuclear and cellular hypertrophy. The nuclear envelope eventually ruptures and fragments, after which viral-induced membranes are assembled along planes through the cell and around its periphery. Subsequently, these membranes coalesce, partitioning most of the cell, including viroplasms and virions in various stages of assembly, among a cluster of vesicles. The vesicles dissociate and are liberated into the hemolymph where they accumulate in large numbers (>108 vesicles per ml), causing the blood to become opaque white. The virus has been isolated from T. ni and transmitted per os and by injection to T. ni and several other species of the family Noctuidae. The virions produced by this virus are large (ca. 130 X 400 nm), enveloped, and allantoid in shape with complex symmetry and contain apparently linear, double-stranded DNA of M, of 1.00 x 108. The envelope contains subunits arranged in a hexagonal pattern that impart a distinctive reticular appearance to virions in negatively stained preparations. The unique structural and developmental properties of this virus indicate that it is a member of a group of enveloped, double-stranded DNA viruses not observed previously.An unusual type of virus not observed previously has been found causing a chronic but ultimately fatal disease in larvae of the noctuid moths Scotogramma trifolii (1), Trichoplusia ni (2), and Heliothis zea (3) (order, Lepidoptera; family, Noctuidae). The most unusual feature of this virus is that it induces the host cell to cleave into a cluster of virion-containing vesicles. These vesicles are highly refractile, typically range in diameter from 2 to 12 gm and, after cleavage of the host cell, dissociate and are liberated into the hemolymph where they accumulate in large numbers (>108 vesicles per ml) as the disease progresses. An additional unusual feature of this virus is the unique structure of the virion, which is a large enveloped particle, essentially allantoid in shape, with an envelope that exhibits a distinctive hexagonal pattern in negatively stained preparations.In two initial reports on the disease caused by this virus, the identity of the pathogen was not confirmed (1, 2). It was suggested that the causative agent was either a new type of virus or, because of its large size, shape, and occurrence in membrane-bound vesicles, a peculiar type of Rickettsia. A more definitive characterization of the disease in S. trifolii was not undertaken because attempts to transmit the virus in the laboratory were unsuccessful. However, the occurrence of the virus in populations of T. ni throughout the year permitted the collection of diseased insects on a regular basis and enabled the devel...

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Section: Resultsmentioning

confidence: 99%

Enveloped double-stranded DNA insect virus with novel structure and cytopathology

Federici

1983

Proc. Natl. Acad. Sci. U.S.A.

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“…These open reading frames were evaluated using published mRNA mapping data (20,23,24), by analysis of codon usage compared with known HSV genes (25), and by comparisons with corresponding sequences from the genomes of varicella-zoster virus (VZV) and Epstein-Barr virus (EBV). VZV is, like HSV, a member of the alphaherpesvirinae sub-family (26) (26,27).…”

Section: Methodsmentioning

confidence: 99%

DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

1986

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“…The Rhabdovirideae represent one of only two virus families with members that infect and cause serious diseases in both plants and animals (1). The members of the family infect hosts that are widely distributed throughout both kingdoms and have been isolated from many species of plants, mammals, fish, insects, and other invertebrates (2, 3).…”

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confidence: 99%

Physical map of the genome of sonchus yellow net virus, a plant rhabdovirus with six genes and conserved gene junction sequences.

Heaton

Hillman

Hunter

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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We provide evidence that a plant rhabdovirus, sonchus yellow net virus (SYNV), is similar to most animal rhabdoviruses in the order of structural genes and in the nucleotide sequences at the gene junctions but that it differs in the presence and location of a putative nonstructural gene. From the patterns of hybridization of a library of recombinant DNA clones, we have shown that the SYNV genome is transcribed into a short 3'-terminal "leader RNA" and six mRNAs. The proteins encoded by the SYNV mRNAs, in order of the appearance of their genes in the SYNV genome, are designated 3'-N-

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Cited by 581 publications

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Enveloped double-stranded DNA insect virus with novel structure and cytopathology

Enveloped double-stranded DNA insect virus with novel structure and cytopathology

DNA sequence of the region in the genome of herpes simplex virus type 1 containing the exonuclease gene and neighbouring genes

Physical map of the genome of sonchus yellow net virus, a plant rhabdovirus with six genes and conserved gene junction sequences.

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