Tissue-/stage-dependent expression of a cloned Bombyx mandarina QM homologue

Hwang, Jae‐Sam; Goo, Tae-Won; Yun, Eun‐Young; Lee, Jeong‐Hae; Kang, Seok-Woo; Kim, Keon-Young; Kwon, O‐Yu

doi:10.1016/s1389-0344(00)00056-3

Cited by 14 publications

(18 citation statements)

References 19 publications

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“…The QM-like gene has been widely reported in organisms from plants to mammals (Chavez-Rios and Vargas-Mejia, 2000;Hwang et al, 2000;Green et al, 2000). Studies of the QM-like gene in aquatic organisms have focused on shellfishes (Oh et al, 2010), crustaceans (Xu et al, 2008;Zhou et al, 2011), and freshwater fishes (Wen et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)

Chen¹,

Su²,

Wang³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. In order to investigate the immune role of ribosomal protein L10 (RPL10/QM-like gene) in marine fish, we challenged the large yellow croaker Pseudosciaena (= Larimichthys) crocea, the most important marine fish culture species in China, by injection with a mixture of the bacteria Vibrio harveyi and V. parahaemolyticus (3:1 in volume). Microarray analysis and real-time PCR were performed 24 and 48 h post-challenge to isolate and identify the QM-like gene from the gill P. crocea (designated PcQM). The expression level of the PcQM gene did not changed significantly at 24 h post-challenge, but was significantly downregulated at 48 h post-challenge, suggesting that the gene had an immune-modulatory effect in P. crocea. Full-length PcQM cDNA and genomic sequences were obtained by rapid amplification of cDNA ends (RACE)-PCR. The sequence of the PcQM gene clustered together with those of other QM-like genes from other aquatic organisms, indicating that the QM-like gene is highly conserved in teleosts.

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Section: Discussionmentioning

confidence: 99%

Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)

Chen¹,

Su²,

Wang³

et al. 2012

Genet. Mol. Res.

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“…Likewise, the crystallographic structural similarity observed among RPL10 orthologs in eukaryotes, bacteria, and archaea (called L16) established the conservation of this universal ribosomal protein family and provided evidence of the inalterability of the ribosome during evolution (Spahn et al, 2001;Nishimura et al, 2008). Nevertheless, besides being a constituent of ribosomes and participating in protein translation, additional extraribosomal functions have been described for RPL10 (Mills et al, 1999;Hwang et al, 2000;Chávez-Rios et al, 2003;Zhang et al, 2004;Singh et al, 2009). In yeast, RPL10 is essential for viability, organizes the union site of the aminoacyl-tRNA, and its incorporation into the 60S subunit is a prerequisite for subunit joining and the initiation of translation Hofer et al, 2007).…”

mentioning

confidence: 93%

“…Since then, RPL10 has been studied in different organisms from archaea and bacteria to eukaryotes such as mammals, insects, yeast, and plants (Marty et al, 1993;Mills et al, 1999;Hwang et al, 2000;Zhang et al, 2004;Wen et al, 2005;Singh et al, 2009). A remarkable property of this protein is its high degree of amino acid conservation, suggesting fundamental and critical conserved functions of RPL10 in different organisms (Farmer et al, 1994;Eisinger et al, 1997;Hofer et al, 2007;Nishimura et al, 2008).…”

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confidence: 99%

New Evidence for Differential Roles of L10 Ribosomal Proteins from Arabidopsis

et al. 2013

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The RIBOSOMAL PROTEIN L10 (RPL10) is an integral component of the eukaryotic ribosome large subunit. Besides being a constituent of ribosomes and participating in protein translation, additional extraribosomal functions in the nucleus have been described for RPL10 in different organisms. Previously, we demonstrated that Arabidopsis (Arabidopsis thaliana) RPL10 genes are involved in development and translation under ultraviolet B (UV-B) stress. In this work, transgenic plants expressing Pro RPL10 :b-glucuronidase fusions show that, while AtRPL10A and AtRPL10B are expressed both in the female and male reproductive organs, AtRPL10C expression is restricted to pollen grains. Moreover, the characterization of double rpl10 mutants indicates that the three AtRPL10s differentially contribute to the total RPL10 activity in the male gametophyte. All three AtRPL10 proteins mainly accumulate in the cytosol but also in the nucleus, suggesting extraribosomal functions. After UV-B treatment, only AtRPL10B localization increases in the nuclei. We also here demonstrate that the three AtRPL10 genes can complement a yeast RPL10 mutant. Finally, the involvement of RPL10B and RPL10C in UV-B responses was analyzed by two-dimensional gels followed by mass spectrometry. Overall, our data provide new evidence about the nonredundant roles of RPL10 proteins in Arabidopsis.

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“…In addition, it has been demonstrated that the yeast Qm homologous genes, Grc5 and Qsr1, participate in translational control of gene expression in yeast and are required for cell growth and differentiation throughout mRNA translation; deletion of Qsr1 is lethal (Tron et al, 1995;Koller et al, 1996). In animals, the expression of Qm is higher in rapidly dividing tissues than in adult and differentiated tissues, implying that QM may regulate development and differentiation (Green et al, 2000;Hwang et al, 2000). Similar expression patterns were described in the silkworm Bombyx mandarina, where Qm displays a tissue/stagedependent expression (Hwang et al, 2000).…”

mentioning

confidence: 99%

“…In animals, the expression of Qm is higher in rapidly dividing tissues than in adult and differentiated tissues, implying that QM may regulate development and differentiation (Green et al, 2000;Hwang et al, 2000). Similar expression patterns were described in the silkworm Bombyx mandarina, where Qm displays a tissue/stagedependent expression (Hwang et al, 2000). In addition, in the plant species Nicotiana tabacum, a transcript homologous to Qm is highly expressed in young tissues and is almost undetectable in mature tissues, suggesting its contribution in cell growth beyond the role in ribosome structure (Marty et al, 1993).…”

mentioning

confidence: 99%

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

et al. 2010

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(J.B., A.L.B.) Ribosomal protein L10 (RPL10) proteins are ubiquitous in the plant kingdom. Arabidopsis (Arabidopsis thaliana) has three RPL10 genes encoding RPL10A to RPL10C proteins, while two genes are present in the maize (Zea mays) genome (rpl10-1 and rpl10-2). Maize and Arabidopsis RPL10s are tissue-specific and developmentally regulated, showing high levels of expression in tissues with active cell division. Coimmunoprecipitation experiments indicate that RPL10s in Arabidopsis associate with translation proteins, demonstrating that it is a component of the 80S ribosome. Previously, ultraviolet-B (UV-B) exposure was shown to increase the expression of a number of maize ribosomal protein genes, including rpl10. In this work, we demonstrate that maize rpl10 genes are induced by UV-B while Arabidopsis RPL10s are differentially regulated by this radiation: RPL10A is not UV-B regulated, RPL10B is down-regulated, while RPL10C is up-regulated by UV-B in all organs studied. Characterization of Arabidopsis T-DNA insertional mutants indicates that RPL10 genes are not functionally equivalent. rpl10A and rpl10B mutant plants show different phenotypes: knockout rpl10A mutants are lethal, rpl10A heterozygous plants are deficient in translation under UV-B conditions, and knockdown homozygous rpl10B mutants show abnormal growth. Based on the results described here, RPL10 genes are not redundant and participate in development and translation under UV-B stress.

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Tissue-/stage-dependent expression of a cloned Bombyx mandarina QM homologue

Cited by 14 publications

References 19 publications

Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)

Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)

New Evidence for Differential Roles of L10 Ribosomal Proteins from Arabidopsis

Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress

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