Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: The role of the C- and N-terminal extensions

Bando, Toshikazu; Ruksana, Razia; Anokye‐Danso, Frederick; Takashima, Yasuo; Sakube, Yasuji; Kagawa, Hiroaki

doi:10.1016/j.bbapap.2007.01.003

Cited by 8 publications

(9 citation statements)

References 31 publications

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“…The in vivo rescue experiments (Fig. 3) confirm the results from the previous in vitro molecular interaction analysis (Ruksana et al, 2005;Amin et al, 2007). Briefly, TNI-3 and TNI-4 did not rescue animal motility, indicating that TNI-3 is a primitive body-wall type derived from the pharyngeal muscle type TNI-4.…”

Section: Relations Between Cell Fate and Genetic Lineagesupporting

confidence: 84%

“…Comparison between tni-2 and tni-3 clearly show that the head enhancer CBF1/ Su(H)/LAG-1-binding sequence occurs in tni-3 but not tni-2. These differences come from the gene duplication pro- Assessments of the functional complementation among three body-wall TNIs by recovery of motility in unc-27(e155) null mutant worms The TNI-1, TNI-2 and TNI-3 isoforms are classified as body-wall muscle type by their expression pattern, amino acid sequence homology and cross-reactivity to other troponin components (Ruksana et al, 2005;Kagawa et al, 2007;Amin et al, 2007). However, their expression patterns are fairly different as was observed above (Fig.…”

Section: Expression Patterns Of Three Isoforms Of Body-wall Tni Genesmentioning

confidence: 62%

“…Since tni-3 is highly expressed in the body-wall muscles of the head region, TNI-3 may also function for the periodic movement of head, which awaits future studies. Pharyngeal muscle type tni-4 did not rescue motility because TNI-4 does not interact with other body-wall muscle components including TNC-1, TNT-1 and TMI (Ruksana et al, 2005;Amin et al, 2007). The in vivo rescue experiments (Fig.…”

Section: Relations Between Cell Fate and Genetic Lineagementioning

confidence: 92%

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Expression profiles and unc-27 mutation rescue of the striated muscle type troponin I isoform-3 in Caenorhabditis elegans

et al. 2012

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Transcription control of multiple genes in tissue-and stage-specific patterns is still of major interest. We show here that troponin I (TNI) is expressed under the control of upstream non-coding sequences and had functions as an isoform of intermediate type between pharynx and body-wall of the gene. In Caenorhabditis elegans, three striated muscle TNIs are expressed in body-wall muscles and a cardiac isoform is expressed in the pharynx. We have analyzed the gene expression mechanisms of tni-3 gene and motility function of its protein product. Promoter deletion analysis of the tni-3 gene identified muscle enhancers including the head enhancer. The CBF1/Su(H)/LAG-1-binding motif was included in the head enhancer. Yeast one-hybrid screening isolated the lag-1 clone in five candidates. Functional differences between the three striated muscle TNIs were investigated by the expression of promoter-fusion genes into tni-2/unc-27(e155) null mutant animals. The results suggest that the cis-elements in the promoters of the three genes are important for their tissue-specific expression and that from the function of TNI-3, the tni-3 gene would be an intermediate in the evolution of these genes by gene duplication. Mechanisms of tni-3 expression and its molecular function may contribute to our understanding of gene evolution and developmental programs.

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Section: Relations Between Cell Fate and Genetic Lineagesupporting

confidence: 84%

Section: Expression Patterns Of Three Isoforms Of Body-wall Tni Genesmentioning

confidence: 62%

Section: Relations Between Cell Fate and Genetic Lineagementioning

confidence: 92%

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Expression profiles and unc-27 mutation rescue of the striated muscle type troponin I isoform-3 in Caenorhabditis elegans

et al. 2012

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“…25,26 In Fig plasmids showed widespread activity of lacZ in almost all cells when heat-shocked (Fig. 8B).…”

Section: Elt-2 and Pha-4 Directly Interact In Vitromentioning

confidence: 91%

“…25,26 Worm extract was prepared by collecting mixed-stage worms and sonicated in PBS (pH=7.4). Equal volume of 2x Laemmli was added and boiled at 95°C for 2 minutes.…”

Section: C G T G a T A T C A T G G A T A A T A A C T A C A A T G A mentioning

confidence: 99%

Transcription Factors GATA/ELT-2 and Forkhead/HNF-3/PHA-4 Regulate the Tropomyosin Gene Expression in the Pharynx and Intestine of Caenorhabditis elegans

Anokye‐Danso

Anyanful

Sakube

et al. 2008

Journal of Molecular Biology

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SummaryGene regulation during development is an important biological activity that leads to synthesis of biomolecules at specific locations and specific times. The single tropomyosin gene of Caenorhabditis elegans, tmy-1/lev-11 produces four isoforms of protein: two from the external promoter and two from the internal promoter. We investigated the internal promoter of tropomyosin to identify sequences that regulate expression of tmy-1 in the pharynx and intestine. By promoter deletion of tmy-1 reporters as well as by database analyses, a 100 bp fragment was identified that contained binding sequences for a GATA factor, for a chicken CdxA homolog and for a forkhead factor. Both the forkhead and CdxA binding sequences contributed to pharyngeal and intestinal expression. In addition, the GATA site also influenced intestinal expression of tmy-1 reporter. We showed that ELT-2 and PHA-4 proteins interact directly with the GATA and forkhead binding sequences, respectively, in gel mobility shift assays. RNAi knockdown of elt-2 diminished tmy-1::gfp expression in the intestine. In contrast to RNAi knockdown of pha-4, expression of tmy-1::gfp in pha-4;smg-1 mutants was slightly weaker to that of the wild type. Ectopic expression of PHA-4 and ELT-2 by heat shock were sufficient to elicit widespread expression of tmy-1::lacZ reporter in embryos. We found no indication of a synergistic relation between ELT-2 and PHA-4. Based on our data, PHA-4 and CdxA function as general transcription factors for pharyngeal and intestinal regulation of tmy-1. We present models by which ELT-2, PHA-4 and CdxA orchestrate expression from the internal promoter of tmy-1.3

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Regulation of Structure and Function of Sarcomeric Actin Filaments in Striated Muscle of the Nematode Caenorhabditis elegans

Ono

2014

The Anatomical Record

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The nematode Caenorhabditis elegans has been used as a valuable system to study structure and function of striated muscle. The body wall muscle of C. elegans is obliquely striated muscle with highly organized sarcomeric assembly of actin, myosin, and other accessary proteins. Genetic and molecular biological studies in C. elegans have identified a number of genes encoding structural and regulatory components for the muscle contractile apparatuses, and many of them have counterparts in mammalian cardiac and skeletal muscles or striated muscles in other invertebrates. Applicability of genetics, cell biology, and biochemistry has made C. elegans an excellent system to study mechanisms of muscle contractility and assembly and maintenance of myofibrils. This review focuses on the regulatory mechanisms of structure and function of actin filaments in the C. elegans body wall muscle. Sarcomeric actin filaments in C. elegans muscle are associated with the troponin-tropomyosin system that regulates the actin-myosin interaction. Proteins that bind to the side and ends of actin filaments support ordered assembly of thin filaments. Furthermore, regulators of actin dynamics play important roles in initial assembly, growth, and maintenance of sarcomeres. The knowledge acquired in C. elegans can serve as bases to understand the basic mechanisms of muscle structure and function.

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Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans: The role of the C- and N-terminal extensions

Cited by 8 publications

References 31 publications

Expression profiles and <i>unc-27</i> mutation rescue of the striated muscle type troponin I isoform-3 in <i>Caenorhabditis elegans</i>

Expression profiles and <i>unc-27</i> mutation rescue of the striated muscle type troponin I isoform-3 in <i>Caenorhabditis elegans</i>

Transcription Factors GATA/ELT-2 and Forkhead/HNF-3/PHA-4 Regulate the Tropomyosin Gene Expression in the Pharynx and Intestine of Caenorhabditis elegans

Regulation of Structure and Function of Sarcomeric Actin Filaments in Striated Muscle of the Nematode Caenorhabditis elegans

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