Tissue-Specific Gene Expression of Head and Neck Squamous Cell Carcinoma In Vivo by Complementary DNA Microarray Analysis

Abstract

Complementary DNA microarray analysis of human HNSCCAs has produced a preliminary, comprehensive database of tumor-specific gene expression profiles and provided important insights into modeling gene expression changes implicated in carcinogenesis. A large-scale analysis of gene expression carries the future potential of identifying sensitive molecular markers for early tumor detection, prognosis, and novel targets for interceptive therapeutics.

Gene expression profiling of head and neck squamous cell carcinoma using cDNA microarrays

“…In our study we could identify 22 differentially expressed genes out of 408 genes investigated. This result of 5.4% is within limits and close to the mean value of other reported studies with a range from 0.3 to 37.8% differentially expressed genes (mean value 9.2%) (4)(5)(6)(7)(8)(9)(10)(11)(12)(36)(37)(38). However, the meaning of this observation remains unclear, since the amount of differentially expressed genes depends on various factors, such as the array type used.…”

“…These results are supported by the results of our study, while CAPN7 was upregulated in our study as well. All other transcripts reported in this study, have not been described to show differences comparing HNSCC tumor tissue to healthy mucosal tissue by previous publications (4)(5)(6)(7)(8)(9)(10)(11)(12)(36)(37)(38) and therefore represent new findings in the field of HNSCC research.…”

Tumor matrix protein collagen XIα1 in cancer

“…Similar to the above findings, in a microarray study of head and neck squamous cell carcinoma (HNSCC), colXIα1 was one of two genes of 12,000 studied found to be highly upregulated (by an average of 476-fold) in all HNSCC samples studied, and had no expression in normal tissue, making it an ideal marker for HNSCC 39 . This finding was confirmed by another study in which colXIα1 was upregulated by an average of 6.63 fold in HNSCC tumors versus normal adjacent tissue 40 .…”

Tumor‐specific genetic expression profile of metastatic oral squamous cell carcinoma

“…Interestingly, Schmalbach et al also identified significant TGM3 downregulation in metastatic OSCC tumors when compared with both nonmetastatic tumors and unmatched normal oral mucosa 24. The finding of significant TGM3 mRNA downregulation in studies of OSCC metastasis is consistent with results of a number of studies of HNSCC,14–22 as well as a couple studies of esophageal SCC,25, 26 suggesting that a decrease in TGM3 may be important in squamous cell carcinoma tumorigenesis and/or metastasis.…”