Tissue-specific expression and regulation of the haemolymph clottable protein of tiger shrimp (Penaeus monodon)

Yeh, Maw-Sheng; Huang, Chang‐Jen; Cheng, Jin-Hwa; Tsai, Inn-Ho

doi:10.1016/j.fsi.2006.10.007

Cited by 26 publications

(9 citation statements)

References 30 publications

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“…Blood coagulation in shrimp has been proposed to be similar to that in the crayfish, P. leniusculus [40], where under an external stimulus, such as LPS, STG is released from the hyaline hemocytes into the plasma to initiate the blood clotting by cross-linking the CPs, which are abundant proteins in the hemolymph that are mainly produced in the gill, central nervous system and the lymphoid organ [52], [53]. The result of this report here demonstrated that Pm A2M is contained in semigranular hemocytes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Shrimp Alpha-2-Macroglobulin Prevents the Bacterial Escape by Inhibiting Fibrinolysis of Blood Clots

Chaikeeratisak¹,

Somboonwiwat²,

Tassanakajon

2012

PLoS ONE

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Proteomic analysis of the hemocytic proteins of Penaeus monodon (Pm) has previously shown that alpha-2-macroglobulin (A2M) was among the proteins that showed substantially altered expression levels upon Vibrio harveyi infection. Therefore, in this study its potentially important role in the response of shrimp to bacterial infection was further characterized. The yeast two-hybrid system revealed that the receptor binding domain of PmA2M interacted with the carboxyl-terminus of one or both of the transglutaminase type II isoforms, which are key enzymes involved in the shrimp clotting system. In accord with this, PmA2M was found to be localized on the extracellular blood clots and to colocalize with clottable proteins. RNA interference (RNAi)-mediated knockdown of A2M transcript levels reduced the PmA2M transcript levels (∼94%) and significantly reduced the bacterial seizing ability of the clotting system, resulting in an up to 3.3-fold higher number of V. harveyi that systemically disseminated into the circulatory system at 5 min post-infection before subsequent clearance by the immune system. Furthermore, an appearance of PmA2M depleted clots in the presence of V. harveyi strikingly demonstrated fibrinolysis zones surrounding the bacteria. This study provides the first evidence of the vital role of PmA2M in enhancing bacterial sequestration by protecting blood clots against fibrinolysis.

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Section: Discussionmentioning

confidence: 99%

Shrimp Alpha-2-Macroglobulin Prevents the Bacterial Escape by Inhibiting Fibrinolysis of Blood Clots

Chaikeeratisak¹,

Somboonwiwat²,

Tassanakajon

2012

PLoS ONE

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“…Therefore, even though it was suggested [10] that all LS may be formed de novo from hemocytes aggregating in the hemal sinuses, we believe that the LT origin of LS formation could not be ruled out. The compositions and possible functions of the released granular materials from hemocytes could partly be enzymes that break down viruses, while the rest could be proteins that were involved in the coagulation [19][20][21][22] and walling off (encapsulating) the spheroids, thus preventing viral particles and debris from the infected apoptotic cells from spreading. To ensure that this is the case, spheroids were surrounded by a thickened capsule which was made up of argyrophilic type 2 fibers (Fig.…”

Section: Discussionmentioning

confidence: 99%

Changes in the histological organization and spheroid formation in lymphoid organ of Penaeus monodon infected with yellow head virus

Duangsuwan

Tinikul

Chotwiwatthanakun

et al. 2008

Fish & Shellfish Immunology

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“…As demonstrated by previously published literatures, shrimp plasma proteins such as coagulogen, haemocyanin and transglutaminase, were seriously affected after infection by pathogens. In addition, the expression of immune response genes in shrimp was also changed (Song, Yu, Lien, Huang & Lin 2003;Liu, Li, Wang, Dong, Zhang, Luan, Zhang & Xiang 2007;Yeh, Huang, Cheng & Tsai 2007;Yeh, Liu, Hung & Cheng 2009;Wang, Tseng, Lin, Chen, Chen, Chen, Chen & Yang 2010;Liu, Chang, Chiu, Cheng & Yeh 2011).…”

Section: Discussionmentioning

confidence: 99%

Effects ofVibrio harveyion plasma clotting protein (coagulogen) of white shrimpLitopenaeus vannamei

et al. 2015

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Blood clotting exhibits various important functions, including the prevention of body fluid loss and invasion of pathogens in shrimp. The effects of pathogenic Vibrio harveyi on plasma of white shrimp (Litopenaeus vannamei) in vitro and in vivo were investigated in this study. The clotting protein (coagulogen) in plasma of white shrimp pre‐incubated with extracellular products (ECP) of V. harveyi was found apparently decreased and fast‐migrated in crossed immunoelectrophoresis (CIE) gels. In addition, the coagulogen had been degraded to many low molecular‐weight protein bands in plasma pre‐incubated with ECP on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) gels. When pre‐challenged with bacterial cells and ECP of V. harveyi, the white shrimp began to die at about 30 and 16 h respectively. Moreover, plasma coagulogen was decreased more obvious in shrimp challenged with ECP than that with bacterial cells as visualized in CIE gels, and total plasma protein in both group of shrimp were all decreased. Haemolymph withdrawn from moribund shrimp pre‐challenged with V. harveyi or its ECP was observed unclottable. However, the addition of clotting factors (transglutaminase and/or Ca2+) to these unclottable plasma could apparently promote their re‐clotting ability as jelly‐like solid observed in microtubes. The recovery of clotting ability of plasma from moribund shrimp was due to the reformation of coagulogen (200 kDa) after adding the two clotting factors as shown on CIE and SDS‐PAGE gels. The present results suggest that the infection of V. harveyi in white shrimp may not only degrade coagulogen but also influence the presence of transglutaminase and Ca2+ ion.

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Tissue-specific expression and regulation of the haemolymph clottable protein of tiger shrimp (Penaeus monodon)

Cited by 26 publications

References 30 publications

Shrimp Alpha-2-Macroglobulin Prevents the Bacterial Escape by Inhibiting Fibrinolysis of Blood Clots

Shrimp Alpha-2-Macroglobulin Prevents the Bacterial Escape by Inhibiting Fibrinolysis of Blood Clots

Changes in the histological organization and spheroid formation in lymphoid organ of Penaeus monodon infected with yellow head virus

Effects ofVibrio harveyion plasma clotting protein (coagulogen) of white shrimpLitopenaeus vannamei

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