Tissue-specific alternative splicing of Shaker potassium channel transcripts results from distinct modes of regulating 3? splice choice

Iverson, Linda E.; Mottes, John R.; Yeager, Stephanie A.; Germeraad, Susan E.

doi:10.1002/(sici)1097-4695(199705)32:5<457::aid-neu2>3.0.co;2-4

Cited by 12 publications

(5 citation statements)

References 33 publications

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“…The differential ability of CD8‐GFP‐Sh to localize to the prothoracic NMJs raises the possibility that different isoforms of Shaker and/or DLG may be present at the NMJ of muscle 27 as compared to other cervical and coxal muscles. It is well established that Shaker is alternatively spliced to yield a number of isoforms (Kamb et al, 1988; Pongs et al, 1988; Schwarz et al, 1988; Mottes and Iverson, 1995) and that different Shaker isoforms are expressed in the larval bodywall and one set of adult muscles, the dorsolongitudinal flight muscles (Iverson et al, 1997). Whether similar or different Shaker isoforms are present at other adult muscles is not known.…”

Section: Discussionmentioning

confidence: 99%

Morphology and molecular organization of the adult neuromuscular junction of Drosophila

Rivlin

Clair

Vilinsky

et al. 2003

J of Comparative Neurology

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While the larval neuromuscular junction (NMJ) of Drosophila has emerged as a model system to study synaptic function and development, little attention has been given to the study of the adult NMJ. Here we report an immunocytochemical and morphological characterization of an adult NMJ preparation of the prothorax. All muscles examined were innervated by small, uniform type II terminals (0.5-1.5 microm), a subset of which contained octopamine. Terminals classified as type I varied in their morphology across different muscles, ranging from strings or clusters of boutons (0.8-5.5 microm) to an elongate terminal (80-100 microm long) with few branches and contiguous swellings (3-15 microm) along its length. Analysis of the molecular composition of the NMJs during the first 5 days after eclosion revealed four major findings: 1) type I boutons increase in size during early adulthood; 2) Fasciclin II-immunoreactivity is not detectable at type I terminals, while DLG-immunoreactivity is observed at the synapse; 3) a Shaker-GFP fusion protein that localizes to all type I boutons in the larva is differentially localized at adult prothoracic NMJs; and 4) while all type I terminals contain glutamate, the glutamate receptor subunits, DGluRIIA and DGluRIIB, are expressed and clustered in only a subset of muscles. These findings suggest that maturation of the adult NMJ occurs during early adulthood and that muscle-specific properties may play a role in organizing synaptic components in the adult. Furthermore, these results demonstrate that there are major differences in the molecular organization of the adult and larval NMJs.

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Section: Discussionmentioning

confidence: 99%

Morphology and molecular organization of the adult neuromuscular junction of Drosophila

Rivlin

Clair

Vilinsky

et al. 2003

J of Comparative Neurology

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“…In addition, channel heterogeneity resulting from alternative splicing of α‐subunit has been described [30]. N‐ and C‐terminal are known to play important roles for the formation of homo‐ and heteromultimers [31, 32].…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

et al. 1997

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We describe here (1) the heterogeneous expression of Ca 2+ -independent transient (A-type) K + channel K K-subunits (Kv1.4, Kv3.3, Kv3.4, Kv4.2 and Kv4.3) in rat smooth muscle, heart and brain, (2) the molecular cloning and tissue distribution of a novel alternatively spliced variant of an A-type K + channel K K-subunit, Kv4.3, and (3) The longer splice variant is very weakly expressed in brain, but is the major product in heart. z 1997 Federation of European Biochemical Societies.

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“…For example, Calcitonin has been shown to be differentially spliced in the thyroid and the brain, producing a hormone precursor in the former and a neuropeptide in the latter (Rosenfeld et al, 1983). In flies, the Shaker potassium channel has also been shown to produce tissue-specific transcripts with distinct physiological properties (Iverson et al, 1997;Mottes and Iverson, 1995). In comparison, reports of cell-specific alternative splicing have been less common, probably due to the technical difficulties involved in visualizing these splicing events.…”

Section: Neuronmentioning

confidence: 99%

Cell-Specific Alternative Splicing of Drosophila Dscam2 Is Crucial for Proper Neuronal Wiring

Lah

Millard

2014

Neuron

102

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How a finite number of genes specify a seemingly infinite number of neuronal connections is a central question in neurobiology. Alternative splicing has been proposed to increase proteome diversity in the brain. Here we show that cell-specific alternative splicing of a cell-surface protein is crucial for neuronal wiring. Down syndrome cell adhesion molecule 2 (Dscam2) is a conserved homophilic binding protein that can induce repulsion between opposing neurons. In the fly visual system, L1 and L2 neurons both require Dscam2 repulsion, but paradoxically, they also physically contact each other. We found that the cell-specific expression of two biochemically distinct alternative isoforms of Dscam2 prevents these cells from repelling each other. Phenotypes were observed in the axon terminals of L1 and L2 when they expressed the incorrect isoform, demonstrating a requirement for distinct isoforms. We conclude that cell-specific alternative splicing is a mechanism for achieving proper connectivity between neurons.

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Tissue-specific alternative splicing of Shaker potassium channel transcripts results from distinct modes of regulating 3? splice choice

Cited by 12 publications

References 33 publications

Morphology and molecular organization of the adult neuromuscular junction of Drosophila

Morphology and molecular organization of the adult neuromuscular junction of Drosophila

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat

Cell-Specific Alternative Splicing of Drosophila Dscam2 Is Crucial for Proper Neuronal Wiring

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Tissue-specific alternative splicing of Shaker potassium channel transcripts results from distinct modes of regulating 3? splice choice

Cited by 12 publications

References 33 publications

Morphology and molecular organization of the adult neuromuscular junction of Drosophila

Morphology and molecular organization of the adult neuromuscular junction of Drosophila

Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K+ channel α‐subunit, Kv4.3 in the rat

Cell-Specific Alternative Splicing of Drosophila Dscam2 Is Crucial for Proper Neuronal Wiring

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Molecular cloning and tissue distribution of an alternatively spliced variant of an A‐type K⁺ channel α‐subunit, Kv4.3 in the rat