Tissue response to a new type of biomaterial implanted subcutaneously in rats

Boennelycke, Marie; Christensen, Lise; Nielsen, Lene Rostgaard; Everland, Hanne; Lose, Gunnar

doi:10.1007/s00192-010-1257-3

Cited by 16 publications

(12 citation statements)

References 28 publications

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“…By using an abdominal wall defect model in rats, we confirmed our previous findings [11] that the MPEG-PLGA scaffold is very biocompatible and disappears completely in 8 weeks. Desmin-positive muscle cells that were separated from the ordinary muscle layers of the abdominal wall were observed, and fluorescence-positive cells could be traced after 8 weeks, indicating survival and proliferation of cells originating from the MFF.…”

Section: Discussionsupporting

confidence: 75%

“…The MPEG-PLGA scaffold is a freeze-dried combination of glycolic acid and lactic acid, which makes the structure porous and spongy as previously described [11].…”

Section: Scaffoldsmentioning

confidence: 99%

“…Methoxypolyethyleneglycol-polylactic-co-glycolic acid (MPEG-PLGA) is a synthetic, biodegradable scaffold with high biocompatibility [11], which has been used successfully as a carrier for MFF [9].…”

Section: Introductionmentioning

confidence: 99%

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Muscle fragments on a scaffold in rats: a potential regenerative strategy in urogynecology

et al. 2015

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Section: Discussionsupporting

confidence: 75%

“…The MPEG-PLGA scaffold is a freeze-dried combination of glycolic acid and lactic acid, which makes the structure porous and spongy as previously described [11].…”

Section: Scaffoldsmentioning

confidence: 99%

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Muscle fragments on a scaffold in rats: a potential regenerative strategy in urogynecology

et al. 2015

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“…10 At 3 weeks postimplantation, inflammatory scores were qualitatively assessed as 3 or moderate and by 8 weeks had decreased to 1 or minimal. 10,33 However, our inflammatory response was more similar to the acellular skeletal muscle scaffolds implanted into the skeletal muscle of mice reported by Perniconi et al They found that there was an overt inflammatory response 2 weeks postimplantation, but by 4 weeks it had decreased toward control levels. 9 Longer studies are necessary to determine whether the inflammation caused by the implantation of the scaffold continues to decrease over time.…”

Section: Discussionsupporting

confidence: 79%

In Vivo Skeletal Muscle Biocompatibility of Composite, Coaxial Electrospun, and Microfibrous Scaffolds

Fischer

Rossmeisl

Whittington

et al. 2014

Tissue Engineering Part A

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One weakness with currently researched skeletal muscle tissue replacement is the lack of contraction and relaxation during the regenerative process. A biocompatible scaffold that can act similar to the muscle would be a pivotal innovation. Coaxial electrospun scaffolds, capable of movement with electrical stimulation, were created using poly(e-caprolactone) (PCL), multiwalled carbon nanotubes (MWCNT), and a (83/17 or 40/60) poly(acrylic acid)/poly(vinyl alcohol) (PAA/PVA) hydrogel. The two scaffolds were implanted into SpragueDawley rat vastus lateralis muscle and compared with a phosphate-buffered saline injection sham surgery and an unoperated control. No complications or adverse effects were observed. Rats were sacrificed on days 7, 14, 21, and 28 postimplantation and biocompatibility assessed using enzymatic activity, fibrosis formation, inflammation, scaffold cellular infiltration, and neovascularization. Serum creatine kinase and lactate dehydrogenase levels were significantly higher in scaffold-implanted rats compared with the control on day 7, but returned to baseline by day 14. Day 7 scaffolds showed significant inflammation and fibrosis that decreased over time. Fibroblasts infiltrated the scaffolds early, but decreased with time, while myogenic cell numbers increased. Neovascularization of both scaffolds occurred as early as day 7. We conclude that the PCL-MWCNT-PAA/PVA scaffolds are biocompatible and suitable for muscle regeneration as myogenic cell growth was supported.

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“…Apart from the fact that different parts of the meshes are studied for histology making comparisons difficult [26, 27] more relevant is the problem of subjectivity during the scoring process. In the current study, a software detected the positive staining on each section on the same set of mesh filaments and calculated the percentage stained area.…”

Section: Discussionmentioning

confidence: 99%

A preclinical evaluation of polypropylene/polylacticacid hybrid meshes for fascial defect repair using a rat abdominal hernia model

et al. 2017

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ObjectivesSynthetic mesh surgery for both abdominal and urogenital hernia repair is often unsatisfactory in the long-term due to postoperative complications. We hypothesized that a semi-degradable mesh hybrid may provide more appropriate biocompatibility with comparable mechanical properties. The aim was to compare its in vivo biocompatibility with a commercial polypropylene (PP) mesh.Methods72 rats were randomly allocated to either our new composite mesh (monofilament PP mesh knitted with polylactic-acid-fibers (PLA)) or to a commercially available PP mesh that was used as a control. 15, 90, and 180 days after implantation into the rat abdomen mesh tissue complexes were analysed for erosion, contraction, foreign body reaction, tissue integration and biomechanical properties.ResultsNo differences were seen in regard to clinical parameters including erosion, contraction or infection rates between the two groups. Biomechanical properties including breaking load, stiffness and deformation did not show any significant differences between the different materials at any timepoint. Macrophage staining did not reveal any significant differences between the two groups or between timepoints either. In regard to collagen I there was significantly less collagen I in the PP group compared to the PP/ PLA group at day 180. Collagen III did not show any significant differences at any timepoint between the two groups.ConclusionA PP/PLA hybrid mesh, leaving a low amount of PP after PLA degradation seems to have comparable biomechanical properties like PP at 180 days due to enhanced collagen production without significant differences in erosion, contraction, herniation or infection rates.

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Tissue response to a new type of biomaterial implanted subcutaneously in rats

Abstract: The MPEG-PLGA is highly biocompatible, degrades quickly, and seems inert in the process of tissue regeneration. Thus, it is hardly a candidate per se in reinforcement of pelvic reconstruction, but it could have a future role as carrier for stem cells.

Cited by 16 publications

References 28 publications

Muscle fragments on a scaffold in rats: a potential regenerative strategy in urogynecology

Muscle fragments on a scaffold in rats: a potential regenerative strategy in urogynecology

In Vivo Skeletal Muscle Biocompatibility of Composite, Coaxial Electrospun, and Microfibrous Scaffolds

A preclinical evaluation of polypropylene/polylacticacid hybrid meshes for fascial defect repair using a rat abdominal hernia model

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