Tissue processing of oral biopsy specimens: An adjunct to diagnosis

Sathyakumar, M; Premkumar, Jeyanthi; Magesh, Thiyagarajan Karuppur; Martin, Yakob; Thirumlaisamy, Elangovan

doi:10.4103/2278-9588.113592

Cited by 3 publications

(1 citation statement)

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“…The incisional biopsies were obtained from the most representative area of the lesion. The biopsied tissue was then fixed in 10% neutral buffered formalin and sent to the laboratory of the Oral Pathology department, Faculty of Dentistry, Alexandria University, where it was processed, embedded in paraffin wax and stained with Hematoxylin and Eosin for histologic grading of the dysplastic lesions (27).…”

Section: Methodsmentioning

confidence: 99%

Chemopreventive Effect of Topical Application of S-Allylcysteine in the Management of Oral Dysplastic Potentially Malignant Disorders

Meshri

Zaki

Raslan

et al. 2017

Alexandria Dental Journal

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INTRODUCTION:Oral potentially malignant disorders (OPMDs) describe mucosal disorders with an increased risk of malignant transformation (MT) to oral squamous cell carcinoma (OSCC). Natural products like garlic represent a promising group of chemopreventive agents. Aged garlic extract (AGE) is one of the most commonly used garlic preparations as it contains more stable organosulfur compounds (OSCs); S-allylcysteine (SAC) is the most abundant organosulfur compound in AGE. SAC has been found to retard the growth of chemically induced and transplantable tumors in several animal models. OBJECTIVES: To evaluate the cancer chemopreventive effect of topically applied S-Allylcysteine in the management of oral dysplastic potentially malignant disorders. MATERIALS AND METHODS:10 subjects with oral dysplastic potentially malignant disorders, as proven clinically and histopathologically, were recruited for this study. They received topical S-Allylcysteine for 1 month, and then the lesions were evaluated both clinically and histopathologically after termination of therapy to assess any alterations in the lesions' size, pain score and mucosal dysplasia. RESULTS: S-allylcysteine was well tolerated by all the patients. After termination of the therapeutic phase (after one month), S-Allylcysteine was found to decrease the pain score in all symptomatic patients. The size of the lesions was also decreased although it was not statistically significant; however, histological improvement was remarkable. Complete histological response was observed in four leukoplakia patients and two lichen planus patients where the mild and moderate dysplastic changes showed histologic remission of dysplasia. However, two leukoplakia cases showed progression in the grade of dysplasia from mild to moderate. CONCLUSIONS: Topical application of S-Allylcysteine is beneficial for the management of dysplasia associated with oral potentially malignant disorders.

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Section: Methodsmentioning

confidence: 99%

Chemopreventive Effect of Topical Application of S-Allylcysteine in the Management of Oral Dysplastic Potentially Malignant Disorders

Meshri

Zaki

Raslan

et al. 2017

Alexandria Dental Journal

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Conventional paraffin embedding technique and double-embedding technique for minute oral biopsies and delicate pulp tissue — a comparative study

Sundararajan,

Muthusamy,

Prasad

et al. 2024

Rus. J. Arch. Patol.

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Comparison Between Xylene And Coconut Oil In Tissue Processing

Chandraker¹,

Rathod²,

Chandraker³

et al. 2019

Mod Med Lab J

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Background: Clearing is an important step in the preparation of histological sections, that removes alcohol and other dehydrants from tissues prior to infiltration of the embedding material (usually paraffin wax).Different types of clearing agents are chloroform, Xylene, Toluene, Paraffin, Methyl benzoate and methyl salicylate & Citrus fruit oils.The commonly used clearing agent is xylene that is miscible with both alcohol & parrafin wax. Xylene is supposed to be highly toxic and carcinogenic. As previous research studies have shown the effectiveness of different vegetable oils as clearants, this study was designed to evaluate the efficacy of coconut oil. Materials and Methods: Two equal halves of 25 oral soft tissue specimens were processed simultaneously in xylene and coconut oil as clearing agents. The Xylenetreated specimens (XY-S) and Coconut oil-treated specimens (CO-S) were checked for gross and histological features and comparison was done between the two groups. Results: Significant shrinkage was noted in XY-S compared to that in COS. No difference was found in either of the sections when checked for cellular details and staining quality. Morphometrically, there was significant reduction in the mean cell area in XY-S compared to that in COS. Conclusion: Coconut oil may be substituted for the highly hazardous xylene as a clearing agent without compromising the quality of histological details.

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Tissue processing of oral biopsy specimens: An adjunct to diagnosis

Cited by 3 publications

References 0 publications

Chemopreventive Effect of Topical Application of S-Allylcysteine in the Management of Oral Dysplastic Potentially Malignant Disorders

Chemopreventive Effect of Topical Application of S-Allylcysteine in the Management of Oral Dysplastic Potentially Malignant Disorders

Conventional paraffin embedding technique and double-embedding technique for minute oral biopsies and delicate pulp tissue — a comparative study

Comparison Between Xylene And Coconut Oil In Tissue Processing

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