Tissue microarrays compared with whole sections and biochemical analyses. A subgroup analysis of DBCG 82 b&amp;c.

Kyndi, Marianne; Sørensen, Flemming Brandt; Knudsen, Helle; Overgaard, Marie; Nielsen, Hanne Melgaard; Andersen, Jeremiah J.; Overgaard, Jens

doi:10.1080/02841860701851871

Cited by 73 publications

(57 citation statements)

References 21 publications

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“…From the 532 FFPE samples with sufficient tumor tissue, RNA extraction were performed and expression of ESR1, PGR, ERBB2 and MKI67 were quantified with quantitative real time polymerase chain reaction (described in detail in Supplementary Material, available online at http://www.informahealthcare.com [13,[25][26][27]). If the quantification failed, data from medical records and immunohistochemistry (IHC) was used to determinate ER-, PR-and HER2-status.…”

Section: Methodsmentioning

confidence: 99%

Impact of age, intrinsic subtype and local treatment on long-term local-regional recurrence and breast cancer mortality among low-risk breast cancer patients

et al. 2016

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Section: Methodsmentioning

confidence: 99%

Impact of age, intrinsic subtype and local treatment on long-term local-regional recurrence and breast cancer mortality among low-risk breast cancer patients

et al. 2016

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“…With multiplex cores, several reports have indicated that there is reasonable representation of tissue heterogeneity. 14,15 LeBaron et al 16 also have suggested a method for creating TAs without coring the blocks. With this method, the spot for each case is a tiny square; thus, the advantages of the Spiral Array technique described above cannot be achieved.…”

Section: Commentmentioning

confidence: 99%

Spiral Array: A New High-Throughput Technology Covers Tissue Heterogeneity

Fukuoka¹,

Hofer²,

Hori³

et al. 2012

Archives of Pathology &Amp; Laboratory Medicine

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Context.—Tissue array is a well-established technique that connects basic research with clinical applications and allows for the validation of many pathobiologic events from gene expression dysregulation to genomic aberrations. However, conventional tissue array has several limitations such as poor representation of tissue heterogeneity, destruction of donor tissue blocks due to coring, and usage of particular specimens that have limited evaluable material (tissue from thin specimens or needle biopsies). Objective.—To show the noninferiority and superiority of the new technique named Spiral Array—which allows for improved representation of the donor tissue while keeping the architectural details of the donor block intact—to that of the conventional tissue array. We compared the morphologic features of both methods. Design.—We created both Spiral Array and conventional tissue array for 25 lung adenocarcinomas and 50 multiple tumors of various organs. The degree of coverage of tissue heterogeneity was examined by observing the range of the staining intensity differences in immunohistochemistry, using cytokeratin 7 and epidermal growth factor receptor (EGFR); the degree of morphologic preservation was tested by level of accurate prediction among 3 pathologists of the histopathologic diagnosis and organ type. Results.—The Spiral Array showed better representations of the range of staining intensity for EGFR (P = .01). The level of accuracy for predicting organ type was significantly higher in Spiral Array than conventional tissue array (P = .047), whereas it was not significantly different between the 2 techniques for the histologic diagnosis. Conclusion.—Our data indicate that Spiral Array has benefits for covering tissue heterogeneity and preserving better morphology.

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“…Kidney tissue was placed in the first core of the upper left and right corner of the TMA block to ensure correct orientation when examining the slides. All analyses were performed with the use of TMAs, allowing semi-quantitative analysis [21,22].…”

Section: Preparation Of Tissue Microarraysmentioning

confidence: 99%

“…A suitable method for evaluation of marker proteins is immunohistochemistry (IHC) with the use of Tissue Micro Arrays (TMA). TMAs allow rapid, cost-effective and standardized analyses with the use of only a small amount of paraffin embedded tumor tissue and it has made immunohistochemical characterization of protein expression in large series of tumors easier and less laborious [21]. Furthermore, TMAs can be used for semi-quantitative analysis of various potential predictive marker proteins [22].…”

mentioning

confidence: 99%

An ER activity profile including ER, PR, Bcl-2 and IGF-IR may have potential as selection criterion for letrozole or tamoxifen treatment of patients with advanced breast cancer

Henriksen

Rasmussen²,

Lykkesfeldt

et al. 2009

Acta Oncologica

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Tissue microarrays compared with whole sections and biochemical analyses. A subgroup analysis of DBCG 82 b&c.

Abstract: (2008) Tissue microarrays compared with whole sections and biochemical analyses. A subgroup analysis of DBCG 82 b&c., Acta Oncologica, 47:4, 591-599,

Cited by 73 publications

References 21 publications

Impact of age, intrinsic subtype and local treatment on long-term local-regional recurrence and breast cancer mortality among low-risk breast cancer patients

Impact of age, intrinsic subtype and local treatment on long-term local-regional recurrence and breast cancer mortality among low-risk breast cancer patients

Spiral Array: A New High-Throughput Technology Covers Tissue Heterogeneity

An ER activity profile including ER, PR, Bcl-2 and IGF-IR may have potential as selection criterion for letrozole or tamoxifen treatment of patients with advanced breast cancer

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