Tissue engineering strategies for nervous system repair

Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering

“…Guidance by substratum contours has been widely investigated due to the importance of topographical guidance in the tissue development, including establishing precursor migratory patterns and guiding axonal trajectories in the developing nervous system [3,7,18,19]. The results of this investigation showed that the aligned nanofibers (300 nm) could improve the NSC differentiation and support the neurite outgrowth as compared to other electrospun fibers.…”

Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering

“…Guidance by substratum contours has been widely investigated due to the importance of topographical guidance in the tissue development, including establishing precursor migratory patterns and guiding axonal trajectories in the developing nervous system [3,7,18,19]. The results of this investigation showed that the aligned nanofibers (300 nm) could improve the NSC differentiation and support the neurite outgrowth as compared to other electrospun fibers.…”

Enhancement of biocompatibility on aligned electrospun poly(3‐hydroxybutyrate) scaffold immobilized with laminin towards murine neuroblastoma Neuro2a cell line and rat brain‐derived neural stem cells (mNSCs)

“…After consecutive spinning for 8 hours, the thickness of the fibrous scaffold was 364 ± 10 μm. As mentioned, the sole use of scaffolding materials may not be sufficient for regeneration process due to its hydrophobicity surface, likely as a result of the lack of chemical cues that help to promote the cellular ingrowth . One approach to solve this problem, polymer surface modification may potentially be used to enhance the cell‐scaffold interaction.…”

“…As mentioned, the sole use of scaffolding materials may not be sufficient for regeneration process due to its hydrophobicity surface, likely as a result of the lack of chemical cues that help to promote the cellular ingrowth. [3][4][5][6] One approach to solve this problem, polymer surface modification may potentially be used to enhance the cell-scaffold interaction. In the present work, the potential use of surface modified electrospun PHB fibrous scaffold in neural tissue engineering was preliminarily evaluated in vitro towards murine neuroblastoma Neuro2a cell line (ATCC, CCL-131).…”

PC12 neuron-like cell response to electrospun poly( 3-hydroxybutyrate) substrates

“…The limited self‐regenerative capability of both central and peripheral nervous tissue has required the elaboration of different strategies with the purpose of recovering structure and function of damaged nerves (Tresco, ). Some highly promising strategies consist in the transplantation of dopamine secreting cells to improve parkinsonian symptoms and the application of polymeric conduits to direct neurite outgrowth in transected nerves (Cao et al ., ; Yoshida et al ., ).…”

Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering

“…Guidance by substratum contours has been widely investigated due to the importance of topographical guidance in the tissue development, including establishing precursor migratory patterns and guiding axonal trajectories in the developing nervous system [3,7,18,19]. The results of this investigation showed that the aligned nanofibers (300 nm) could improve the NSC differentiation and support the neurite outgrowth as compared to other electrospun fibers.…”

Enhancement of biocompatibility on aligned electrospun poly(3‐hydroxybutyrate) scaffold immobilized with laminin towards murine neuroblastoma Neuro2a cell line and rat brain‐derived neural stem cells (mNSCs)

“…After consecutive spinning for 8 hours, the thickness of the fibrous scaffold was 364 ± 10 μm. As mentioned, the sole use of scaffolding materials may not be sufficient for regeneration process due to its hydrophobicity surface, likely as a result of the lack of chemical cues that help to promote the cellular ingrowth . One approach to solve this problem, polymer surface modification may potentially be used to enhance the cell‐scaffold interaction.…”

“…As mentioned, the sole use of scaffolding materials may not be sufficient for regeneration process due to its hydrophobicity surface, likely as a result of the lack of chemical cues that help to promote the cellular ingrowth. [3][4][5][6] One approach to solve this problem, polymer surface modification may potentially be used to enhance the cell-scaffold interaction. In the present work, the potential use of surface modified electrospun PHB fibrous scaffold in neural tissue engineering was preliminarily evaluated in vitro towards murine neuroblastoma Neuro2a cell line (ATCC, CCL-131).…”

PC12 neuron-like cell response to electrospun poly( 3-hydroxybutyrate) substrates

“…The limited self‐regenerative capability of both central and peripheral nervous tissue has required the elaboration of different strategies with the purpose of recovering structure and function of damaged nerves (Tresco, ). Some highly promising strategies consist in the transplantation of dopamine secreting cells to improve parkinsonian symptoms and the application of polymeric conduits to direct neurite outgrowth in transected nerves (Cao et al ., ; Yoshida et al ., ).…”

Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering

“…Guidance by substratum contours has been widely investigated due to the importance of topographical guidance in the tissue development, including establishing precursor migratory patterns and guiding axonal trajectories in the developing nervous system [3,7,18,19]. The results of this investigation showed that the aligned nanofibers (300 nm) could improve the NSC differentiation and support the neurite outgrowth as compared to other electrospun fibers.…”

Enhancement of biocompatibility on aligned electrospun poly(3‐hydroxybutyrate) scaffold immobilized with laminin towards murine neuroblastoma Neuro2a cell line and rat brain‐derived neural stem cells (mNSCs)

“…After consecutive spinning for 8 hours, the thickness of the fibrous scaffold was 364 ± 10 μm. As mentioned, the sole use of scaffolding materials may not be sufficient for regeneration process due to its hydrophobicity surface, likely as a result of the lack of chemical cues that help to promote the cellular ingrowth . One approach to solve this problem, polymer surface modification may potentially be used to enhance the cell‐scaffold interaction.…”

“…As mentioned, the sole use of scaffolding materials may not be sufficient for regeneration process due to its hydrophobicity surface, likely as a result of the lack of chemical cues that help to promote the cellular ingrowth. [3][4][5][6] One approach to solve this problem, polymer surface modification may potentially be used to enhance the cell-scaffold interaction. In the present work, the potential use of surface modified electrospun PHB fibrous scaffold in neural tissue engineering was preliminarily evaluated in vitro towards murine neuroblastoma Neuro2a cell line (ATCC, CCL-131).…”

PC12 neuron-like cell response to electrospun poly( 3-hydroxybutyrate) substrates

“…The limited self‐regenerative capability of both central and peripheral nervous tissue has required the elaboration of different strategies with the purpose of recovering structure and function of damaged nerves (Tresco, ). Some highly promising strategies consist in the transplantation of dopamine secreting cells to improve parkinsonian symptoms and the application of polymeric conduits to direct neurite outgrowth in transected nerves (Cao et al ., ; Yoshida et al ., ).…”