Tissue Engineering Skeletal Muscle

Kosnik, Paul E.; Dennis, Robert G.; Vandenburgh, Herman H.

doi:10.1007/0-387-21547-6_28

Cited by 20 publications

(20 citation statements)

References 100 publications

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“…2,14 Powell and others found that repetitive stretch and relaxation equal to 10% of length, six times per hour, increased differentiation into myotubes. 15 Yuge and Kataoka seeded myoblasts with magnetic microparticles and induced differentiation by placing them in a magnetic field, without adding special growth factors or any conditioned medium.…”

Section: Fieldsmentioning

confidence: 99%

“…By introducing a variety of environmental cues, these cells fuse into myotubes, which can then differentiate into myofibers. 2 The resulting myofibers may then be harvested, cooked, and consumed as meat. van Eelen, van Kooten, and Westerhof hold a Dutch patent for this general approach to producing cultured meat.…”

Section: Cultured Meat Productionmentioning

confidence: 99%

“…7 Attempts to force skeletal muscle fibers to proliferate are typically counterproductive, as most myonuclei remain postmitotic. 2 Embryonic stem cells have the drawback that despite the high proliferation and differentiation potential, considerable effort must be applied to force them to differentiate and cell yields from harvests are low. Moreover, it is not clear whether embryonic stem cells forced to commit to a skeletal muscle lineage will have the proliferative characteristics of embryonic stem cells, or become indistinguishable from myoblasts.…”

Section: Cellsmentioning

confidence: 99%

“…16 Electrical stimulation also contributes to differentiation, as well as sarcomere formation within established myotubes. 2,14 …”

Section: Fieldsmentioning

confidence: 99%

“…8 Kosnik, Dennis, and Vandenburgh refer to serum-free media developed by Allen et al, Dollenmeier et al, and Ham et al 2 Benjaminson and others succeeded in using a serum-free medium made from maitake mushroom extract that achieved higher rates of growth than fetal bovine serum. 6 And it has been shown that lipids such as sphingosine 1-phosphate can replace serum in supporting the growth and differentiation of embryonic tissue explants (W.S.…”

Section: Culture Media and Growth Factorsmentioning

confidence: 99%

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Commentary:In Vitro-Cultured Meat Production

et al. 2005

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Section: Fieldsmentioning

confidence: 99%

Section: Cultured Meat Productionmentioning

confidence: 99%

Section: Cellsmentioning

confidence: 99%

“…16 Electrical stimulation also contributes to differentiation, as well as sarcomere formation within established myotubes. 2,14 …”

Section: Fieldsmentioning

confidence: 99%

Section: Culture Media and Growth Factorsmentioning

confidence: 99%

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Commentary:In Vitro-Cultured Meat Production

et al. 2005

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Drug‐screening platform based on the contractility of tissue‐engineered muscle

et al. 2008

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A tissue-based approach to in vitro drug screening allows for determination of the cumulative positive and negative effects of a drug at the tissue rather than the cellular or subcellular level. Skeletal muscle myoblasts were tissue-engineered into three-dimensional muscle with parallel myofibers generating directed forces. When grown attached to two flexible micro-posts (mu posts) acting as artificial tendons in a 96-well plate format, the miniature bioartificial muscles (mBAMs) generated tetanic (active) forces upon electrical stimulation measured with a novel image-based motion detection system. mBAM myofiber hypertrophy and active force increased in response to insulin-like growth factor 1. In contrast, mBAM deterioration and weakness was observed with a cholesterol-lowering statin. The results described in this study demonstrate the integration of tissue engineering and biomechanical testing into a single platform for the screening of compounds affecting muscle strength.

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Synergy between myogenic and non-myogenic cells in a 3D tissue-engineered craniofacial skeletal muscle construct

Brady

Lewis

Mudera

2008

J Tissue Eng Regen Med

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In vitro skeletal muscle engineering involves the culture of isolated primary myogenic cells in an environment conducive to the formation of a three-dimensional (3D) tissue construct capable of generating force. Isolated human myogenic cells have been used to study cell-cell interactions, permitting identification of functions intrinsic to skeletal muscle in two dimensions (2D). However, the independent contribution of human myogenic and non-myogenic cell types that comprise skeletal muscle to myogenic cell differentiation, force generation and matrix remodelling has yet to be established in 3D. The objective of this study was to use isolated human myogenic and non-myogenic muscle-derived cells (MDC) seeded in 3D collagen constructs to engineer a biomimetic craniofacial skeletal construct. The aim was to purify the two subpopulations of myogenic and non-myogenic cells from human masseter muscle and quantitate myogenic cell differentiation, force generation and matrix remodelling of the 3D collagen construct. The results showed that both the heterogeneous mixture of cells and the purified myogenic cell population expressed myogenin, indicative of myogenic cell differentiation. Further, there was a synergistic effect as the heterogeneous co-culture of myogenic and non-myogenic cells generated the highest peak force and expressed the most MMP-2 mRNA compared to isolated individual cell populations.

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Tissue Engineering Skeletal Muscle

Cited by 20 publications

References 100 publications

Commentary:In Vitro-Cultured Meat Production

Commentary:In Vitro-Cultured Meat Production

Drug‐screening platform based on the contractility of tissue‐engineered muscle

Synergy between myogenic and non-myogenic cells in a 3D tissue-engineered craniofacial skeletal muscle construct

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