Tissue engineering of skin and cornea

Paquet, Claudie; Larouche, Danielle; Bisson, Francis; Proulx, Stéphanie; Simard-Bisson, Carolyne; Gaudreault, Manon; Robitaille, Hubert; Carrier, Patrick; Martel, I.; Duranceau, L; Auger, François A.; Fradette, Julie; Guérin, Sylvain L.; Germain, Lucie

doi:10.1111/j.1749-6632.2009.05373.x

Cited by 32 publications

(7 citation statements)

References 78 publications

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“…Studies by Luo et al showed that isolated HUCB stem cells could enhance the healing of mice skin defect wounds, and it was found that the implanted HUCB stem cells could differentiate into keratinocytes in the wound tissue [222]. Cultured ADSCs can also contribute to wound healing and can be added to engineer a trilayered skin substitute featuring the skin's deepest layer, the adipocyte-containing hypodermis, along with both dermis and epidermis [223]. ADSCs secrete various growth factors that control and regenerate damaged skin-type cells, a function that has been termed essential for the regenerative mechanisms of ADSCs [224].…”

Section: Stem Cell-scaffold Constructs For Skin Tissue Engineeringmentioning

confidence: 97%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

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Stem cells are a promising alternative for cell therapy applications because of their self-renewing capability and potential to differentiate into a wide range of specialized cell lineages, including osteoblasts, chondrocytes, cardiomyocytes, and neuronal cells. Different kinds of stem cells are considered for cell-based tissue engineering approaches, of which bone-marrow-derived mesenchymal stem cells (BM-MSCs), adipose-derived stem cells (ADSCs) and embryonic stem cells (ESCs) are frequently utilized for advancement of new tissue engineering strategies. Nanostructures created by natural, synthetic, or composite polymeric biomaterials provide artificial templates of the extracellular matrix (ECM). They possess a high surface area to volume ratio, are porous with good mechanical properties, are sufficient to serve as a biomimetic platform to attract stem cells, cause differentiation, and provide functioning of the tissues. Nanoscale features such as fibers, pits, and grooves modulate cell behavior and might even stimulate the differentiation of stem cells to specific lineages; for example, the nanotopographical cues combined with chemical cues influence the neuronal induction of MSCs, resulting in high microtubule-associated protein expressions. Scaffolds can also be impregnated with several ligands for adhesion of receptors to the cells, or with other regulatory molecules and growth M.P. Prabhakaran (*) and J. Venugopal E3-05-12, Health Care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore e-mail: nnimpp@nus.edu.sg L. Ghasemi-Mobarakeh Islamic Azad University, Najafabad Branch, Isfahan, Iran D. Kai NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore G. Jin and S. Ramakrishna Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576, Singapore factors for designed cell behavior or controlled cell differentiations. Different polymeric blends or fibers incorporated with nanoparticles (such as hydroxyapatite) in aligned or custom-made patterns can induce specific differentiation of stem cells for bone, cartilage, cardiac, nerve, and skin tissue regeneration. In this review, we will discuss the current state of the art and future perspectives on stem cells and their differentiation on nanoengineered substrates for advanced tissue regeneration.

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Section: Stem Cell-scaffold Constructs For Skin Tissue Engineeringmentioning

confidence: 97%

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Prabhakaran

Venugopal

Ghasemi‐Mobarakeh

et al. 2011

Biomedical Applications of Polymeric Nanofibers

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“…Recently, ocular toxicity tests have been increasingly performed with in vitro methods [13]. The authorities have encouraged researchers to develop in vitro studies, for example, the European legislation (Directive 63/2010/EU) is based on replacement, reduction, and refinement of animal experiments.…”

Section: Introductionmentioning

confidence: 99%

Human corneal cell culture models for drug toxicity studies

Rönkkö

Vellonen

Järvinen

et al. 2016

Drug Deliv. and Transl. Res.

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In vivo toxicity and absorption studies of topical ocular drugs are problematic, because these studies involve invasive tissue sampling and toxic effects in animal models. Therefore, different human corneal models ranging from simple monolayer cultures to three-dimensional models have been developed for toxicological prediction with in vitro models. Each system has its own set of advantages and disadvantages. Use of non-corneal cells, inadequate characterization of gene-expression profiles, and accumulation of genomic aberrations in human corneal models are typical drawbacks that decrease their reliability and predictive power. In the future, further improvements are needed for verifying comparable expression profiles and cellular properties of human corneal models with their in vivo counterparts. A rapidly expanding stem cell technology combined with tissue engineering may give future opportunities to develop new tools in drug toxicity studies. One approach may be the production of artificial miniature corneas. In addition, there is also a need to use large-scale profiling approaches such as genomics, transcriptomics, proteomics, and metabolomics for understanding of the ocular toxicity.

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“…We have previously shown that the feeder layer-dependent (i3T3) preservation of the growth characteristics of primary cultured skin keratinocytes and both corneal epithelial and endothelial cells also rely on maintaining the stability of the TFs Sp1 and NFI [14][15][16][17]. Both Sp1 and NFI are well known housekeeping transcription factors that each plays a very important role in cell proliferation [31][32][33][34]. Remarkably, i3T3 were proposed to delay terminal differentiation of human skin keratinocytes by preventing the proteosomal degradation of Sp1 and NFI, both of which play pivotal functions during cell cycle progression [14,33,35].…”

Section: Discussionmentioning

confidence: 99%

Irradiated Human Fibroblasts as a Substitute Feeder Layer to Irradiated Mouse 3T3 for the Culture of Human Corneal Epithelial Cells: Impact on the Stability of the Transcription Factors Sp1 and NFI

Le‐Bel

Ghio

Guérin

et al. 2019

IJMS

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Because of the worldwide shortage of graftable corneas, alternatives to restore visual impairments, such as the production of a functional human cornea by tissue engineering, have emerged. Self-renewal of the corneal epithelium through the maintenance of a sub-population of corneal stem cells is required to maintain the functionality of such a reconstructed cornea. We previously reported an association between stem cell differentiation and the level to which they express the transcription factors Sp1 and NFI. In this study, we investigated the impact of replacing irradiated 3T3 (i3T3) murine fibroblast feeder cells by irradiated human corneal fibroblasts (iHFL) on the expression of Sp1 and NFI and evaluated their contribution to the proliferative properties of human corneal epithelial cells (hCECs) in both monolayer cultures and human tissue engineered corneas (hTECs). hCECs co-cultured with iHFL could be maintained for up to two more passages than when they were grown with i3T3. Western Blot and electrophoretic mobility shift assays (EMSAs) revealed no significant difference in the feeder-layer dependent increase in Sp1 at both the protein and DNA binding level, respectively, between HCECs grown with either i3T3 or iHFL. On the other hand, a significant increase in the expression and DNA binding of NFI was observed at each subsequent passage when hCECs were co-cultured along with i3T3. These changes were found to result from an increased expression of the NFIA and NFIB isoforms in hCECs grown with i3T3. Exposure of hCECs to cycloheximide revealed an increased stability of NFIB that likely resulted from post-translational glycosylation of this protein when these cells were co-cultured with i3T3. In addition, iHFL were as efficient as i3T3 at preserving corneal, slow-cycling, epithelial stem cells in the basal epithelium of the reconstructed hTECs. Furthermore, we observed an increased expression of genes whose encoded products promote hCECs differentiation along several passages in hCECs co-cultured with either type of feeder layer. Therefore, the iHFL feeder layer appears to be the most effective at maintaining the proliferative properties of hCECs in culture most likely by preserving high levels of Sp1 and low levels of NFIB, which is known for its gene repressor and cell differentiation properties.

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Tissue engineering of skin and cornea

Cited by 32 publications

References 78 publications

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Stem Cells and Nanostructures for Advanced Tissue Regeneration

Human corneal cell culture models for drug toxicity studies

Irradiated Human Fibroblasts as a Substitute Feeder Layer to Irradiated Mouse 3T3 for the Culture of Human Corneal Epithelial Cells: Impact on the Stability of the Transcription Factors Sp1 and NFI

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