Tissue Engineering of Lips and Muco-Cutaneous Junctions: <i>In Vitro</i> Development of Tissue Engineered Constructs of Oral Mucosa and Skin for Lip Reconstruction

Peramo, Antonio; Marcelo, Cynthia L.; Feinberg, Stephen E.

doi:10.1089/ten.tec.2011.0406

Cited by 31 publications

(15 citation statements)

References 34 publications

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“…Based on the in vitro behaviour of the keratinocytes, Zajicek et al [ 38 ] suggested that the ADM promotes wound healing through supporting the growth of patient's own keratinocytes from the adnexa remnants in the wound by providing optimal conditions for their attachment, proliferation, and migration. Peramo et al [ 39 ] proved that Alloderm could also permit the differentiation and stratification of nonkeratinized, buccal mucosa in vitro.…”

Section: Discussionmentioning

confidence: 99%

Naturally Occurring Extracellular Matrix Scaffolds for Dermal Regeneration: Do They Really Need Cells?

Eweida

Marei

2015

BioMed Research International

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The pronounced effect of extracellular matrix (ECM) scaffolds in supporting tissue regeneration is related mainly to their maintained 3D structure and their bioactive components. These decellularized matrix scaffolds could be revitalized before grafting via adding stem cells, fibroblasts, or keratinocytes to promote wound healing. We reviewed the online published literature in the last five years for the studies that performed ECM revitalization and discussed the results of these studies and the related literature. Eighteen articles met the search criteria. Twelve studies included adding cells to acellular dermal matrix (ADM), 3 studies were on small intestinal mucosa (SIS), one study was on urinary bladder matrix (UBM), one study was on amniotic membrane, and one study included both SIS and ADM loaded constructs. We believe that, in chronic and difficult-to-heal wounds, revitalizing the ECM scaffolds would be beneficial to overcome the defective host tissue interaction. This belief still has to be verified by high quality randomised clinical trials, which are still lacking in literature.

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Section: Discussionmentioning

confidence: 99%

Naturally Occurring Extracellular Matrix Scaffolds for Dermal Regeneration: Do They Really Need Cells?

Eweida

Marei

2015

BioMed Research International

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“…Para ello Peramo et al; desarrolló una técnica en donde logró que células epiteliales cutáneas y orales sembradas sobre un andamio de AlloDerm migraran y se encontraran para así conformar ésta estructura (10). El análisis de los tejidos obtenidos no mostró expresión de citoqueratina (CK) 4 (marcador de la mucosa labial), pero si mostró una marcada expresión de CK 10 del lado cutáneo y de CK 19 solamente en la capa basal del lado labial.…”

Section: Uniones Muco-cutáneas Y Reconstrucción Labialunclassified

New challenges on the production of oral mucosa using Tissue Engineering Techniques

Bastidas¹,

Bello²

2016

Actual. Med.

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En los últimos años, la fabricación de tejidos artificiales mediante técnicas de Ingeniería Tisular ha sido de gran interés en el área de las ciencias de la salud. En concreto, en el campo odontológico y maxilofacial, la fabricación de una mucosa oral artificial completamente funcional ha sido un reto constante para la Ingeniería Tisular. En este sentido, aunque se han desarrollado diversos modelos de mucosa oral humana, aún existen carencias en lo que se refiere al mimetismo de los tejidos artificiales y los tejidos orales nativos. Dentro de dichas carencias destacan los tiempos prolongados del cultivo de queratinocitos, su baja capacidad de proliferación, la obtención de epitelios delgados y sin crestas epiteliales, la falta de vascularización en la zona implantada y la obtención de tejidos con una unión muco-cutánea para la reconstrucción del labio. El objetivo de esta revisión es mostrar los avances que hasta el momento se han obtenido en materia de la Ingeniería Tisular de la mucosa oral, así como discutir los retos por superar y sus posibles soluciones. AbstractIn recent years, the production of artificial tissues by Tissue Engineering techniques has been of great interest in the health sciences area. Specifically, in the dental and maxillofacial field. In this context, to develop a fully functional artificial oral mucosa is a constant challenge for Tissue Engineering. In this regard, several human oral mucosa models have been developed. However, prolonged periods of keratinocyte culture, low proliferation capacity, absence of rete ridges, and the lack of vascularization are the major shortcomings in this area. The objective of this review is to show the progress that so far have been obtained in the field of Tissue Engineering of the oral mucosa, as well as discuss the challenges ahead and possible solutions. Departamento de Histología, Universidad de GranadaEnviado : 17-09-2016 Revisado: 30-10-2016 Aceptado: 26-12-2016 Juliana Girón Bastidas Teléfono: 57 (1) Actual. Med. 2016; 101: (799): 176-182 INTRODUCCIÓNA pesar de que se han logrado grandes avances en la Ingeniería Tisular (IT) de la mucosa oral, hasta el momento no ha sido posible elaborar una mucosa oral generada por Ingeniería Tisular (MOIT) con todas las características funcionales propias de la mucosa oral humana, con un epitelio organizado, bien diferenciado y con una adecuada vascularización. En este contexto, se han detectado diferentes problemas en torno a la producción de una mucosa oral artificial, como por ejemplo los largos tiempos de cultivo para obtener queratinocitos, la baja capacidad de proliferación de las células epiteliales, la obtención de epitelios delgados y sin formación de crestas epiteliales, la falta de vascularización que conlleva al fracaso del implante y la obtención de tejidos con una unión mucocutánea para ser empleados en la reconstrucción del labio. El objetivo de esta revisión es mostrar los avances que hasta el momento se han obtenido en materia de la Ingeniería Tisular de la mucosa oral, así como discu...

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“…Typically, such junctions involve transitioning of epithelium to epidermis, lamina propia to dermis and smooth muscle to skeletal muscle. An in vitro tissue engineered human lip-like element reproducing the transitional morphology of human lip including epidermal skin, vermillion and oral mucosa has recently been reported (Peramo et al, 2012). This observation notwithstanding, it should be appreciated that the regeneration of a muco-cutaneous junction at the skin/conduit interface of a synthetic neo-organ such as the NUC has never before existed in nature.…”

Section: Regeneration Of Muco-cutaneous Region At Skin-conduit Junctionmentioning

confidence: 99%

An Organ Regeneration Platform for Industrial Production of Hollow Neo-Organs

Basu¹

2014

Cells and Biomaterials in Regenerative Medicine

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Tissue Engineering of Lips and Muco-Cutaneous Junctions: In Vitro Development of Tissue Engineered Constructs of Oral Mucosa and Skin for Lip Reconstruction

Cited by 31 publications

References 34 publications

Naturally Occurring Extracellular Matrix Scaffolds for Dermal Regeneration: Do They Really Need Cells?

Naturally Occurring Extracellular Matrix Scaffolds for Dermal Regeneration: Do They Really Need Cells?

New challenges on the production of oral mucosa using Tissue Engineering Techniques

An Organ Regeneration Platform for Industrial Production of Hollow Neo-Organs

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