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Cited by 208 publications
(127 citation statements)
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“…Ex vivo‐based scaffolds hold the biological ECM composition of native tissues: a complex site‐specific combination of biochemical and biomechanical cues challenging to synthetically reproduce. Its complexity is key for tissue regeneration as it provides a specific nanotopography as well as signaling molecules known to guide cell behavior and further tissue development (Garreta et al, ). Safe and effective decellularization techniques have been developed that allow for removal of immunogenic cellular materials while preserving the nonimmunogenic ECM architecture and bioactive factors (Damodaran & Vermette, ; Gilpin & Yang, ; Porzionato et al, ).…”
Section: Discussionmentioning
confidence: 99%
“…Ex vivo‐based scaffolds hold the biological ECM composition of native tissues: a complex site‐specific combination of biochemical and biomechanical cues challenging to synthetically reproduce. Its complexity is key for tissue regeneration as it provides a specific nanotopography as well as signaling molecules known to guide cell behavior and further tissue development (Garreta et al, ). Safe and effective decellularization techniques have been developed that allow for removal of immunogenic cellular materials while preserving the nonimmunogenic ECM architecture and bioactive factors (Damodaran & Vermette, ; Gilpin & Yang, ; Porzionato et al, ).…”
Section: Discussionmentioning
confidence: 99%
“…However, further improvements are necessary to generate mature functional nephron structures with vascularized glomeruli, and connected to a collecting duct system. In this regard, novel bioengineering approaches using decellularized matrices, synthetic materials and co-culture techniques could contribute to enhance the maturation of 3D kidney structures by providing controlled microenvironments [40][41][42].…”
Section: Discussionmentioning
confidence: 99%
“…Of note, hP-SC-derived kidney organoids may benefit from novel bioengineering approaches to re-create with fidelity the hierarchical architecture of the kidney in vitro by means of biomimetic biomaterials, micro/nanotechnology, microfluidic devices and bioreactors [40,41]. Recently, organ decellularization has opened new perspectives in tissue engineering by providing organ-specific scaffolds that may allow transplanted cells to retain their phenotypic properties, and enhance their maturation [42]. To date, the usage of acellular kidney extracellular matrix promoting differentiation of hPSCs has been reported in few studies, taking advantage of rhesus monkey kidney matrices [43,44].…”
Section: Generating Kidney Organoids From Hpscsmentioning
confidence: 99%
“…One of the most crucial aspects of 3D bioprinting is the preparation of “bioink.” Ink formation process and the detailed understanding of the physical science involving the ink jetting through extremely small diameter nozzles will someday pave the way for clog‐free 3D printing. In the human body, tissues vary remarkably in terms of rigidity ranging from 0.2–5 kPa in soft tissues such as brain to 15,000 kPa in bone (Garreta et al, ). To design patient‐specific 3D analogues, matching the mechanical compliance of host tissue largely depends upon the bioink, its composition, nature, concentration, viscosity, flowability, and several other factors.…”
Section: Bioinkmentioning
confidence: 99%