Tissue engineering and cell therapy of cartilage and bone

Cancedda, Ranieri; Dozin, Béatrice; Giannoni, Paolo; Quarto, Rodolfo

doi:10.1016/s0945-053x(03)00012-x

Cited by 452 publications

(293 citation statements)

References 65 publications

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“…The microenvironment of biomaterial scaffolds is very important for cellular functions, stem cell differentiation, tissue formation and eventually regeneration of injured tissues [3,4]. Scaffolds for tissue engineering should be biocompatible, biodegradable and highly permeable to facilitate mass transport.…”

Section: Introductionmentioning

confidence: 99%

The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes

et al. 2010

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Section: Introductionmentioning

confidence: 99%

The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes

et al. 2010

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“…These differentiated MSC's can then be seeded into a 3-D scaffold made from natural or man-made materials such as collagen, fibrin hydrogels, PLGA/PLLA copolymers 4 , chitosan 5 , silk 6 and others.…”

Section: Introductionmentioning

confidence: 99%

A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

Matcher

2009

Journal of Applied Physics

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This article reviews recent developments in the optical imaging of articular cartilage using polarized-light methods, with an emphasis on tools that could be of use in tissue engineering approaches to treatment. Both second harmonic generation microscopy and polarizationsensitive optical coherence tomography are described and their potential role in the treatment of cartilage disorders such as osteoarthritis is suggested. Key results are reviewed and future developments discussed.2

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“…However, cartilage repair and regeneration are major challenges despite recent progress in orthopedic surgery, because the lesions do not spontaneously heal and their consequences have a significant social and economic impact (Brooks et al, 2002;Sellards et al, 2002). The tissue engineering strategy provides a promising therapy to repair cartilage defects and restore joint functions (Stoddart et al, 2009;Ochi et al, 2001;Cancedda et al, 2003;Tuli et al, 2003). A three-dimensional (3D) scaffold is employed for delivery or recruitment of reparative cells in an organized manner to bridge voids within cartilage defects, offering considerable promise as repair strategies (Solchaga et al, 2001;Grande et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

Liu²,

Guan³

et al. 2009

eCM

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Nano-fibrous scaffolds which could potentially mimic the architecture of extracellular matrix (ECM) have been considered a good candidate matrix for cell delivery in tissue engineering applications. In the present study, a semicrystalline diblock copolymer, poly(ε-caprolactone)-block-poly(L-lactide) (PCL-b-PLLA), was synthesized and utilized to fabricate nano-fibrous scaffolds via a thermally induced phase separation process. Uniform nano-fibrous networks were created by quenching a PCL-b-PLLA/THF homogenous solution to -20ºC or below, followed by further gelation for 2 hours due to the presence of PLLA and PCL microcrystals. However, knot-like structures as well as continuously smooth pellicles appeared among the nanofibrous network with increasing gelation temperature. DSC analysis indicated that the crystallization of PCL segments was interrupted by rigid PLLA segments, resulting in an amorphous phase at high gelation temperatures. Combining TIPS (thermally induced phase separation) with saltleaching methods, nano-fibrous architecture and interconnected pore structures (144±36 μm in diameter) with a high porosity were created for in vitro culture of chondrocytes. Specific surface area and protein adsorption on the surface of the nano-fibrous scaffold were three times higher than on the surface of the solid-walled scaffold. Chondrocytes cultured on the nano-fibrous scaffold exhibited a spherical condrocyte-like phenotype and secreted more cartilage-like extracellular matrix (ECM) than those cultured on the solid-walled scaffold. Moreover, the protein and DNA contents of cells cultured on the nanofibrous scaffold were 1.2-1.4 times higher than those on the solid-walled scaffold. Higher expression levels of collagen II and aggrecan mRNA were induced on the nanofibrous scaffold compared to on the solid-walled scaffold. These findings demonstrated that scaffolds with a nanofibrous architecture could serve as superior scaffolds for cartilage tissue engineering.

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Tissue engineering and cell therapy of cartilage and bone

Cited by 452 publications

References 65 publications

The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes

The chondrogenic differentiation of mesenchymal stem cells on an extracellular matrix scaffold derived from porcine chondrocytes

A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

Microstructure and properties of nano-fibrous PCL-b-PLLA scaffolds for cartilage tissue engineering

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