Tissue distribution and species difference of the brain type glucose transporter (GLUT3)

Yano, Hideki; Seino, Yutaka; Inagaki, Nobuya; Hinokio, Yoshinori; Yamamoto, T.; Yasuda, Koichiro; Masuda, Kouji; Someya, Yoshimichi; Imura, Hiroo

doi:10.1016/0006-291x(91)91440-n

Cited by 90 publications

(44 citation statements)

References 22 publications

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“…Glut1 is almost invariably the major glucose transporter isoform expressed in cultured cells, and as such is responsible for basal glucose transport. Glut3 in turn is the most prominent isoform in the brain (33)(34)(35)(36), but has also been detected in the testis (32,37) and placenta (38). Like Glut1, Glut3 is characterized by a highaffinity dissociation constant (K m ) for glucose (39).…”

Section: Discussionmentioning

confidence: 99%

The midcycle increase in ovarian glucose uptake is associated with enhanced expression of glucose transporter 3. Possible role for interleukin-1, a putative intermediary in the ovulatory process.

et al. 1997

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This study characterizes the rat ovary as a site of hormonally dependent glucose transporter (Glut) expression, and explores the potential role of interleukin (IL)-1, a putative intermediary in the ovulatory process, in this regard. Molecular probing throughout a simulated estrous cycle revealed a significant surge in ovarian Glut3 (but not Glut1) expression at the time of ovulation. Treatment of cultured whole ovarian dispersates from immature rats with IL-1 ␤ resulted in upregulation of the relative abundance of the Glut1 (4.5-fold) and Glut3 (3.5-fold) proteins as determined by Western blot analysis. Other members of the Glut family (i.e., Gluts 2, 4, and 5) remained undetectable. The ability of IL-1 to upregulate Glut1 and Glut3 transcripts proved time-, dose-, nitric oxide-, and protein biosynthesis-dependent but glucose independent. Other ovarian agonists (i.e., TNF ␣ , IGF-I, interferon-␥ , and insulin) were without effect. Taken together, our findings establish the mammalian ovary as a site of cyclically determined Glut1 and Glut3 expression, and disclose the ability of IL-1 to induce the ovarian expression as well as translation of Glut1 and Glut3 (but not of Gluts 2, 4, or 5). Our observations also establish IL-1 as the first known regulator of Glut3, the most efficient Glut known to date. In so doing, IL-1, a putative component of the ovulatory process, may be acting to meet the increased metabolic demands imposed on the growing follicle and the ovulated cumulus-enclosed oocyte. ( J. Clin. Invest. 1997. 99:2274-2283.)

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Section: Discussionmentioning

confidence: 99%

The midcycle increase in ovarian glucose uptake is associated with enhanced expression of glucose transporter 3. Possible role for interleukin-1, a putative intermediary in the ovulatory process.

et al. 1997

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“…Since the nucleotide sequence used for the Northern blot analysis was obtained from the brain transporter cDNA identical with the Glut 1 type, only the 2.8 kb transcript of this particular transporter was monitored, leaving other possible brain glucose transporter transcripts undetected. Human fetal glucose transporter (Glut 3) is reported to be highly expressed in rodent brains (Yano et al, 1991) with transcripts at 4.1 kb and 3.2 kb. Recently, Sadiq et al…”

Section: Discussionmentioning

confidence: 99%

Pattern of glucose transporter (Glut 1) expression in embryonic brains is related to maturation of bood‐brain barrier tightness

Dermietzel

Krause

Kremer

et al. 1992

Developmental Dynamics

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A constant supply of bloodborne glucose is vital to cerebral metabolism. Although transport of glucose into the nervous tissue, effectively separated from the blood by a functional barrier (the blood-brain barrier, BBB), is one of the essential properties of the cerebral endothelium, little is known about its metabolic regulation and developmental expression in the BBB. In this study we provide evidence by immunocytochemistry that the pattern of the brain endothelial glucose transporter in rat brains (BBB-GT), immunologically homologous with the human hepatoma (G2), human erythrocyte transporter (Glut l), changes with BBB maturation. While the neuroepithelium at embryonic days 12 and 13 shows a high incidence of immuno-detectable BBB-GT, vascularisation of the cerebral anlage and subsequent development of vascular tightness, as evidenced by intravascularly applied horseradish peroxidase and fluorescinated dextrans, is accompanied by a significant reduction of BBB-GT expression in neuroepithelial cells and confinement of BBB-GT expression to the cerebral endothelium. Immunoblots and Northern blots of embryonic brain homogenates corroborate this change in BBB-GT expression in the brain anlage at the time of BBB maturation. However, low molecular weight glucose transporters, presumed to be of non-endothelial origin, are less dramatically reduced. The development of BBB tightness, therefore, seems to play a pivotal role in the pattern of BBB-GT expression during brain differentiation.

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“…15 It has 64.4% and 51.6% identity with GLUT1 and GLUT2, respectively, and is expressed mostly in the brain, in particular in the frontal lobe of the cerebrum. 15,[17][18][19] Its primary substrate is also glucose. 15 GLUT14 was identified by Wu and Freeze as a duplicon of GLUT3 with 94.5% identity.…”

Section: Molecular Characterization Of Class I Ii and Iii Glutsmentioning

confidence: 99%

Structure of, and functional insight into the GLUT family of membrane transporters

Long¹,

Cheeseman²

2015

CHC

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This review examines the development of structure and function of the human GLUT proteins, gene family hSLC2A. These proteins are essential for moving the key metabolites, glucose, galactose, and fructose in and out of cells, as well as a number of other important substrates. Despite over five decades of research, it is still not fully understood how they work at the molecular level, although the recent publication of a crystal structure of GLUT1 sug-

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Tissue distribution and species difference of the brain type glucose transporter (GLUT3)

Cited by 90 publications

References 22 publications

The midcycle increase in ovarian glucose uptake is associated with enhanced expression of glucose transporter 3. Possible role for interleukin-1, a putative intermediary in the ovulatory process.

The midcycle increase in ovarian glucose uptake is associated with enhanced expression of glucose transporter 3. Possible role for interleukin-1, a putative intermediary in the ovulatory process.

Pattern of glucose transporter (Glut 1) expression in embryonic brains is related to maturation of bood‐brain barrier tightness

Structure of, and functional insight into the GLUT family of membrane transporters

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