Tissue distribution and excretion of the five components of Portulaca oleracea L. extract in rat assessed by UHPLC

Zhang, Wenjie; Li, Di; Leng, Aijing; Ai, Junjun; Du, Yunchen; Yuan, Meng; Gao, Yucong; Ying, Xixiang

doi:10.1590/s1984-82502015000300016

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…Caffeic acid and ferulic acids levels in the brain were significantly increased after injection of an intravenous bolus of these compounds to rats (Gasperotti et al 2015). Ferulic acid but not caffeic acid was also found in rat brains after oral administration of Portulaca oleracea extract, which contains both compounds (Zhang et al 2015). The detection of these CQAs and metabolites in the brain supports their potential to penetrate the blood brain barrier after administration of C. asiatica , and suggests that in vitro effects seen for some of these compounds on neuronal cultures may be recapitulated in vivo .…”

Section: Absorption Distribution Metabolism and Elimination (Adme) mentioning

confidence: 99%

Centella asiatica: phytochemistry and mechanisms of neuroprotection and cognitive enhancement

et al. 2017

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This review describes in detail the phytochemistry and neurological effects of the medicinal herb Centella asiatica (L.) Urban. C. asiatica is a small perennial plant that grows in moist, tropical and sub-tropical regions throughout the world. Phytochemicals identified from C. asiatica to date include isoprenoids (sesquiterpenes, plant sterols, pentacyclic triterpenoids and saponins) and phenylpropanoid derivatives (eugenol derivatives, caffeoylquinic acids, and flavonoids). Contemporary methods for fingerprinting and characterization of compounds in C. asiatica extracts include liquid chromatography and/or ion mobility spectrometry in conjunction with high-resolution mass spectrometry. Multiple studies in rodent models, and a limited number of human studies support C. asiatica’s traditional reputation as a cognitive enhancer, as well as its anxiolytic and anticonvulsant effects. Neuroprotective effects of C.asiatica are seen in several in vitro models, for example against beta amyloid toxicity, and appear to be associated with increased mitochondrial activity, improved antioxidant status, and/or inhibition of the pro-inflammatory enzyme, phospholipase A2. Neurotropic effects of C. asiatica include increased dendritic arborization and synaptogenesis, and may be due to modulations of signal transduction pathways such as ERK1/2 and Akt. Many of these neurotropic and neuroprotective properties of C.asiatica have been associated with the triterpene compounds asiatic acid, asiaticoside and madecassoside. More recently, caffeoylquinic acids are emerging as a second important group of active compounds in C. asiatica, with the potential of enhancing the Nrf2-antioxidant response pathway. The absorption, distribution, metabolism and excretion of the triterpenes, caffeoylquinic acids and flavonoids found in C. asiatica have been studied in humans and animal models, and the compounds or their metabolites found in the brain. This review highlights the remarkable potential for C. asiatica extracts and derivatives to be used in the treatment of neurological conditions, and considers the further research needed to actualize this possibility.

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Section: Absorption Distribution Metabolism and Elimination (Adme) mentioning

confidence: 99%

Centella asiatica: phytochemistry and mechanisms of neuroprotection and cognitive enhancement

et al. 2017

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“…The triterpenes asiatic acid and madecassic acid (found in C. asiatica ) were detected in mouse brain following their administration as pure compounds ( Yin et al, 2012 ) and their corresponding glycosides asiaticoside and madecassoside were observed in rat brain following administration of a C. asiatica extract or the pure compounds ( Anukunwithaya et al, 2016 ; Hengjumrut et al, 2018 ). Mono and di-CQAs ( Su et al, 2014 ), and their potential metabolites caffeic and ferulic acid have been detected in rat brain after administration of the pure compounds or botanical sources ( Su et al, 2014 ; Gasperotti et al, 2015 ; Zhang et al, 2015 ). The regional distribution of these compounds within the brain has not been reported.…”

Section: Discussionmentioning

confidence: 99%

Amelioration of age-related cognitive decline and anxiety in mice by Centella asiatica extract varies by sex, dose and mode of administration

Gray,

Hack,

Brandes

et al. 2024

Front. Aging

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Background: A water extract (CAW) of the Ayurvedic plant Centella asiatica administered in drinking water has been shown to improve cognitive deficits in mouse models of aging and neurodegenerative diseases. Here the effects of CAW administered in drinking water or the diet on cognition, measures of anxiety and depression-like behavior in healthy aged mice are compared.Methods: Three- and eighteen-month-old male and female C57BL6 mice were administered rodent AIN-93M diet containing CAW (0, 0.2, 0.5 or 1% w/w) to provide 0, 200 mg/kg/d, 500 mg/kg/d or 1,000 mg/kg/d CAW for a total of 5 weeks. An additional group of eighteen-month-old mice were treated with CAW (10 mg/mL) in their drinking water CAW for a total of 5 weeks to deliver the same exposure of CAW as the highest dietary dose (1,000 mg/kg/d). CAW doses delivered were calculated based on food and water consumption measured in previous experiments. In the fourth and fifth weeks, mice underwent behavioral testing of cognition, anxiety and depression (n = 12 of each sex per treatment group in each test).Results: Aged mice of both sexes showed cognitive deficits relative to young mice while only female aged mice showed increased anxiety compared to the young female mice and no differences in depression were observed between the different ages. CAW (1,000 mg/kg/d) in the drinking water improved deficits in aged mice in learning, executive function and recognition memory in both sexes and attenuated the increased measures of anxiety observed in the aged female mice. However, CAW in the diet only improved executive function in aged mice at the highest dose (1,000 mg/kg/d) in both sexes and did so less robustly than when given in the water. There were no effects of CAW on depression-like behavior in aged animals regardless of whether it was administered in the diet or the water.Conclusions: These results suggest that CAW can ameliorate age-related changes in measures of anxiety and cognition and that the mode of administration is important for the effects of CAW on resilience to these age-related changes.

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“…Pharmacological studies demonstrated that the plant possessed many activities including the neuroprotective effect (Ahmed, Moneim, 2013), anti-tumor (Zhao et al, 2013), anti-inflammatory (Meng et al, 2016), woundhealing effect (Rashed et al, 2003), antibacterial (Chan et al, 2015) and hypocholesterolemic (Movahedian, Ghannadi, Vashirnia, 2007).With its popularity, more and more scholars thus pay more attention to its bioactive components, such as terpenes (Xin et al, 2008), phenolic acids and coumarins (Awad, 1994), flavonoids (Xu, Yu, Chen, 2006) and alkaloids (Tian et al, 2014). Also, the pharmacokinetics of some of the bioactive components of P. oleracea L. (Cheng et al, 2012;Zhang et al, 2015) was reported recently.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract

Tao

Gao

et al. 2017

Braz. J. Pharm. Sci.

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Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra-and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%.

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Tissue distribution and excretion of the five components of Portulaca oleracea L. extract in rat assessed by UHPLC

Cited by 3 publications

References 6 publications

Centella asiatica: phytochemistry and mechanisms of neuroprotection and cognitive enhancement

Centella asiatica: phytochemistry and mechanisms of neuroprotection and cognitive enhancement

Amelioration of age-related cognitive decline and anxiety in mice by Centella asiatica extract varies by sex, dose and mode of administration

Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract

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