TiO2 nanoparticles and bulk material stimulate human peripheral blood mononuclear cells

Becker, Kathrin; Schroecksnadel, Sebastian; Geisler, Simon; Carrière, Marie; Gostner, Johanna M.; Schennach, Harald; Herlin, N.; Fuchs, Dietmar

doi:10.1016/j.fct.2013.12.018

Cited by 40 publications

(23 citation statements)

References 77 publications

(60 reference statements)

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“…The cell culture medium normally contains inorganic ions, which can cause the change of radicals on the surface of particles resulting in reduction of the ζ potential (Bhattacharya et al ., ; Miura et al ., ). The decrease in the ζ potential of TiO 2 ‐NPs dispersed in media was also reported in work published previously (Andersson‐Willman et al ., ; Becker et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Cytotoxic and inflammatory responses of TiO₂ nanoparticles on human peripheral blood mononuclear cells

Kongseng

Yoovathaworn

Wongprasert

et al. 2016

J of Applied Toxicology

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Titanium dioxide nanoparticles (TiO2 -NPs) have been widely used in many applications. Owing to their nanoscale size, interactions between cells and NPs have been expansively investigated. With the health concerns raised regarding the adverse effects of these interactions, closer examination of whether TiO2 -NPs can induce toxicity towards human cells is greatly needed. Therefore, in this study, we investigated the cytotoxicity of TiO2 -NPs towards human blood cells (peripheral blood mononuclear cells [PBMCs]) in serum-free medium, for which there is little information regarding the cytotoxic effects of TiO2 -NPs. Our results provide evidence that PBMCs treated with TiO2 -NPs (at concentrations ≥25 μg ml(-1) ) for 24 h significantly reduced cell viability and significantly increased production of toxic mediators such as reactive oxygen species and inflammatory response cytokines such as interleukin-6 and tumor necrosis factor-α (P < 0.05). Cell apoptosis induction also occurred at these concentrations. Significant expressions of cyclooxygenase-2 and interleukin-1β were also observed in PBMCs treated with TiO2 -NPs at concentrations ≥125 μg ml(-1) . Our data presented here clearly indicate that the concentration of TiO2 -NPs (at size ~26.4 ± 1.2 nm) applied to human blood cells has a strong impact on cytotoxic induction. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Resultsmentioning

confidence: 99%

Cytotoxic and inflammatory responses of TiO₂ nanoparticles on human peripheral blood mononuclear cells

Kongseng

Yoovathaworn

Wongprasert

et al. 2016

J of Applied Toxicology

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“…The effect of some nanoparticles on humans is summarized in Table 6. [457][458][459][460][461][462][463][464][465][466] Now, researchers are focusing not only on the new synthesis method of nanoparticles but also showing their concern regarding the fate of nanoparticles and their effects on humans. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Table 6 5.…”

Section: Toxicity Of Cerium Oxide Nanoparticles (Ceo 2 )mentioning

confidence: 99%

“…Recent studies on toxicity of nanoparticles to humans [457][458][460][461][462][463][464][465][466][467] Induced changes in the expression levels of adhesion molecules and The c-x-c chemokine receptor type 4 (cxcr4) in these cells, T-cell proliferation upon cell exposure to TiO 2 and Al 2 O 3 nps…”

mentioning

confidence: 99%

Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles

Srivastava

Gusain

Sharma

2015

Ind. Eng. Chem. Res.

238

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With tremendous increase in development of nanotechnology, there is a developing enthusiasm towards the application of nanoparticles in diverse areas. Carbon nanotubes, fullerenes, quantum dots, dendrimers, iron oxide, silica, gold and silver nanoparticles are frequently used in different applications such as drug delivery, as ceramic materials, semiconductors, electronics, in medicine, cosmetics, etc. Some of these nanoparticles have shown major toxic effects on fauna, flora and human beings like inflammation, cytotoxicity, tissue ulceration and reduction of cell viability. SWCNT and MWCNT can induce oxidative stress and fibrosis in the lungs of rat and mice. SWCNTs can also induce oxidative stress to the nervous system in human beings. Inflammatory injury and respiratory distress can be observed due to TiO 2 nanoparticles with small diameter. Nanoparticles can also pose detrimental effects on plants such as decreased growth rate, genomic and proteomic changes, etc. Toxicity of nanoparticles arises because of their specific characteristics such as greater 'surface area to volume ratio' compared with bulk particles of the same chemistry. The objective of this review is to critically evaluate the current literature on the toxicity of nanoparticles.

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“…Studies of the effects of NP on immune cells are generally scant (Becker et al 2014;Hussain et al 2012). Hence, there remains a pressing need to evaluate effects of various types of NP on immune cell function (Dobrovolskaia & McNeil 2007;Dobrovolskaia et al 2008;Elsabahy & Wooley 2013;Zolnik et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Immunotoxic effects of gold and silver nanoparticles: Inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytesin vitro

Devanabanda

Latheef

Ramanadham

2016

Journal of Immunotoxicology

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Understanding the effects of nanoparticles (NP) on immune cell functions is essential in designing safe and effective NP-based in vivo drug delivery systems. The immunomodulatory potential of gold nanoparticles (GNP) and silver nanoparticles (SNP) was investigated in vitro using murine splenic and human peripheral blood lymphocytes (PBL) in terms of effects on viability and mitogen-induced proliferation. Hydrodynamic size and number of NP were characterized using NP tracking analysis (NTA); modal diameters of GNP and SNP were 28 (±1.5) and 66 (± 2.7) nm, respectively, with a unimodal distribution. Lymphocytes were incubated with GNP or SNP in the presence/absence of B-or T-cell mitogens and proliferative responses then determined using [3 H]-thymidine incorporation. Concanavalin A (T-cell-specific) and lipopolysaccharide-(B-cell-specific) stimulated responses of murine splenic lymphocytes, as well as phytohemagglutinin (T-cell-specific) and pokeweed mitogen-(B-and T-cell specific) induced responses of human lymphocytes, were significantly inhibited by GNP (25-200 lg/ml) and SNP (12.5-50 lg/ml). However, [3 H]-thymidine incorporation by unstimulated lymphocytes was unaffected in the presence of GNP or SNP. Viability of lymphocytes was determined using trypan blue dye exclusion and was significantly inhibited only at 200 lg GNP/ml and 25 or 50 lg SNP/ml. As mitogen responses are most useful to provide supportive mechanistic information on primary immunotoxicologic functional observations, and so far more comprehensive data (in vivo and in vitro) is still needed, the results nevertheless suggest to us that GNP and SNP might potentially be able to modulate immune responses by impacting on lymphocyte activation. ARTICLE HISTORY

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TiO2 nanoparticles and bulk material stimulate human peripheral blood mononuclear cells

Cited by 40 publications

References 77 publications

Cytotoxic and inflammatory responses of TiO₂ nanoparticles on human peripheral blood mononuclear cells

Cytotoxic and inflammatory responses of TiO₂ nanoparticles on human peripheral blood mononuclear cells

Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles

Immunotoxic effects of gold and silver nanoparticles: Inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytesin vitro

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TiO2 nanoparticles and bulk material stimulate human peripheral blood mononuclear cells

Cited by 40 publications

References 77 publications

Cytotoxic and inflammatory responses of TiO2 nanoparticles on human peripheral blood mononuclear cells

Cytotoxic and inflammatory responses of TiO2 nanoparticles on human peripheral blood mononuclear cells

Critical Review on the Toxicity of Some Widely Used Engineered Nanoparticles

Immunotoxic effects of gold and silver nanoparticles: Inhibition of mitogen-induced proliferative responses and viability of human and murine lymphocytesin vitro

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Cytotoxic and inflammatory responses of TiO₂ nanoparticles on human peripheral blood mononuclear cells

Cytotoxic and inflammatory responses of TiO₂ nanoparticles on human peripheral blood mononuclear cells