2020
DOI: 10.1101/2020.04.20.051425
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Time-resolved transcriptome of barley anthers and meiocytes reveals robust and largely stable gene expression changes at meiosis entry

Abstract: In flowering plants, successful germinal cell development and meiotic recombination depend upon a combination of environmental and genetic factors. To gain insights into this specialised reproductive development programme we used short- and long-read RNA-sequencing (RNA-seq) to study the temporal dynamics of transcript abundance in immuno-cytologically staged barley (Hordeum vulgare) anthers and meiocytes. We show that the most significant transcriptional changes occur at the transition from pre-meiosis to lep… Show more

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Cited by 8 publications
(8 citation statements)
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References 114 publications
(112 reference statements)
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“…Ribosomal RNA appeared the most abundant class whereas lncRNA is the less expressed class. This result is consistent with a recent study in barley in which 65% of the downregulated DEGs were lncRNAs in leptotene/zygotene vs. pre-meiosis comparison (Barakate et al, 2020).…”
Section: Rna-seq From Isolated Nuclei Of the Meiocytessupporting
confidence: 93%
“…Ribosomal RNA appeared the most abundant class whereas lncRNA is the less expressed class. This result is consistent with a recent study in barley in which 65% of the downregulated DEGs were lncRNAs in leptotene/zygotene vs. pre-meiosis comparison (Barakate et al, 2020).…”
Section: Rna-seq From Isolated Nuclei Of the Meiocytessupporting
confidence: 93%
“…Paulo Rapazote-Flores and Runxuan Zhang for their Bioinformatic help and suggestions and Mr. Philip Smith for proofreading. An earlier version of this manuscript was released as a pre-print at bioRxiv ( Barakate et al, 2020 ).…”
mentioning
confidence: 99%
“…The expression patterns of rice TDR and the barley orthologue temporally mirror each other. Since TDR is primarily expressed in the tapetum and the barley orthologue expressed in the meiocyte sample, this nding indicates that tapetal tissue was likely included in the sample (the membrane of the 'meiocyte bag') (Barakate et al 2020). Rice TDR is located on chromosome 2, in syntenic position with the barley orthologue located on chromosome 3H (Mayer et al 2011).…”
Section: Discussionmentioning
confidence: 96%