Time resolved Raman spectroscopy for depth analysis of multi‐layered mineral samples

Hooijschuur, Jan-Hein; Petterson, Ingeborg E. Iping; Davies, Gareth R.; Gooijer, C.; Ariese, Freek

doi:10.1002/jrs.4369

Cited by 25 publications

(33 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This research elaborates on our previous work on depth analysis of multilayered mineral samples utilizing TRRS (Hooijschuur et al, 2013) and detection of carotenoids and microorganisms in mineral matrices with RRS (Hooijschuur et al, 2015a). Here, we demonstrate for the first time how two species of microorganisms, D. radiodurans and the cyanobacterium Chroococcidiopsis, can be detected through different layers of translucent and transparent minerals using RRS and TRRS.…”

Section: Introductionmentioning

confidence: 94%

“…To this end, the small prism that steers the excitation beam was moved a few millimeters parallel to the sample surface. For more details and a sketch of the setup, see the work of Hooijschuur et al (2013). The reference spectra of Fig.…”

Section: Measurementsmentioning

confidence: 99%

“…NASA will equip their rover with two Raman instruments: one for the SHERLOC system with 248.6 nm excitation and one for the SuperCam system with 532 nm excitation and gated detection that can be used for stand-off measurements . Since the first proposal to equip a planetary rover with a Raman instrument (Wang et al, 1995), various Raman spectroscopic techniques like remote RS (Angel et al, 2012), resonance Raman spectroscopy (RRS) (Marshall et al, 2007), and time-resolved Raman spectroscopy (TRRS) (Blacksberg et al, 2010;Hooijschuur et al, 2013) have been applied to optimize detection of both minerals and organic compounds.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Raman Spectroscopic Techniques for Planetary Exploration: Detecting Microorganisms through Minerals

Verkaaik¹,

Hooijschuur²,

Davies³

et al. 2015

Astrobiology

Self Cite

View full text Add to dashboard Cite

Raman spectroscopy can provide highly specific chemical fingerprints of inorganic and organic materials and is therefore expected to play a significant role in interplanetary missions, especially for the search for life elsewhere in our solar system. A major challenge will be the unambiguous detection of low levels of biomarkers on a mineral background. In addition, these biomarkers may not be present at the surface but rather inside or underneath minerals. Strong scattering may prevent focusing deeper into the sample. In this paper, we report the detection of carotenoid-containing microorganisms behind mineral layers using time-resolved Raman spectroscopy (TRRS). Two extremophiles, the bacterium Deinococcus radiodurans and the cyanobacterium Chroococcidiopsis, were detected through translucent and transparent minerals using 440 nm excitation under resonance conditions to selectively enhance the detection of carotenoids. Using 3 ps laser pulses and a 250 ps gated intensified CCD camera provided depth selectivity for the subsurface microorganisms over the mineral surface layer and in addition lowered the contribution of the fluorescent background. Raman spectra of both organisms could be detected through 5 mm of translucent calcite or 20 mm of transparent halite. Multilayered mineral samples were used to further test the applied method. A separate tunable laser setup for resonance Raman and a commercial confocal Raman microscope, both with continuous (non-gated) detection, were used for comparison. This study demonstrates the capabilities of TRRS for the depth-selective analysis through scattering samples, which could be used in future planetary exploration to detect microorganisms or biomarkers within or behind minerals.

show abstract

Section: Introductionmentioning

confidence: 94%

Section: Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Raman Spectroscopic Techniques for Planetary Exploration: Detecting Microorganisms through Minerals

Verkaaik¹,

Hooijschuur²,

Davies³

et al. 2015

Astrobiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Full details of the instrumental setup including a schematic representation are given in Hooijschuur et al (2013). TRRS time delays are expressed relative to the laser pulse.…”

Section: Instrumentationmentioning

confidence: 99%

Will Raman meet bacteria on Mars? An overview of the optimal Raman spectroscopic techniques for carotenoid biomarkers detection on mineral backgrounds

Hooijschuur

Verkaaik

Davies

et al. 2015

Netherlands Journal of Geosciences

View full text Add to dashboard Cite

Raman spectroscopy appears to be an ideal technique for the initial detection of biomarkers, molecules that are potentially indicative of life on planetary bodies elsewhere in our solar system. Carotenoids are particularly useful biomarkers as they are used widely across the species, relatively resistant to breakdown and no inorganic source is known. They are used by microorganisms in their cell membranes for protection against UV radiation. In this paper we focus on the detection of carotenoids in microorganisms within a mineral matrix. We compare the Raman signatures of pure compounds with those of laboratory-made mixtures of β-carotene and minerals. Carotenoids covered by 2.5 mm of translucent calcite or 40 mm of transparent halite were detected using a conventional confocal Raman microscope. To improve sensitivity and hence detection levels, Raman measurements were successfully performed under resonant conditions. Raman analysis can be compromised by fluorescence interference. Data are presented to show how the contribution from the fluorescent background in the Raman spectra can be reduced when making use of gated detection in time-resolved Raman spectroscopy. Overall, this study demonstrates some of the potential of Raman spectroscopy as a method for the detection of (past) life signatures during future planetary missions without taking current technical limitations such as instrumental size into account as recent rapid technical developments suggest these limitations will be resolved in time.

show abstract

“…Hooijschuur and coworkers used time‐resolved Raman spectroscopy (TRRS) to carry out depth analysis of multilayered mineral samples. This study establishes the strong potential for the use of Raman spectroscopy in future planetary exploration, where TRRS could be used as a noninvasive tool for profiling the subsurface at millimeter‐depth resolution . Lima and coworkers used 2D‐IR spectroscopy as an additional dimension to investigate ultrafast structural dynamics.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in linear and nonlinear Raman spectroscopy. Part VIII

Nafie

2014

J Raman Spectroscopy

View full text Add to dashboard Cite

The goal of this annual review is to provide an overview of advances in the field of Raman spectroscopy as reflected in articles published each year in the Journal of Raman Spectroscopy as well as in trends across related journals that have published papers in the broad field of Raman spectroscopy. The content is obtained from statistical data on article counts obtained from Thomson Reuters ISI Web of Knowledge by year and by subfield of Raman spectroscopy. Additional information is gleaned from presentations at the XXIV International Conference on Raman Spectroscopy in Germany in August 2014 and those featuring Raman scattering at SCIX 2014 organized by the Federation of Analytical Chemistry and Spectroscopy Societies in Reno, Nevada, USA in October 2014. Coverage is also provided for topics from the conference GeoRaman 2014 held in June in St. Louis, Missouri and ECONOS 2014 held in May in Dole, France. Finally, papers published in JRS in 2013 are highlighted and arranged by topics at the frontier of Raman spectroscopy. From these various perspectives, it is clear that Raman spectroscopy is a rapidly expanding field that provides sensitive photonic information of matter at the molecular level in an ever‐widening sphere of novel applications. Copyright © 2014 John Wiley & Sons, Ltd.

show abstract

Time resolved Raman spectroscopy for depth analysis of multi‐layered mineral samples

Cited by 25 publications

References 35 publications

Raman Spectroscopic Techniques for Planetary Exploration: Detecting Microorganisms through Minerals

Raman Spectroscopic Techniques for Planetary Exploration: Detecting Microorganisms through Minerals

Will Raman meet bacteria on Mars? An overview of the optimal Raman spectroscopic techniques for carotenoid biomarkers detection on mineral backgrounds

Recent advances in linear and nonlinear Raman spectroscopy. Part VIII

Contact Info

Product

Resources

About