Time-resolved nanomechanics of a single cell under the depolymerization of the cytoskeleton

García, Pablo D.; Guerrero, Carlos R.; García, Ricardo García

doi:10.1039/c7nr03419a

Cited by 36 publications

(53 citation statements)

References 57 publications

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“…Several FDC experiments have revealed that the approach and retraction sections of force-distance curves performed on mammalian cells do not overlap. 3,41,50 The hysteresis observed in a FDC is either a consequence the existence of energy-dissipation processes inside a material 59,60 or produced by the breaking of the plasma membrane. Force-distance curves acquired sequentially on a single cell are very reproducible, 3,41 therefore, we conclude that the hysteresis is dominated by dissipative processes occurring inside the cell.…”

Section: Mechanical Model Of a Living Cellmentioning

confidence: 99%

“…31,32 Living cells show a variety of viscoelastic phenomena which remain insufficiently understood. [33][34][35][36][37][38][39][40][41][42][43][44][45][46][47] This lack of understanding has fundamental and practical implications. The accuracy of Young's modulus measurements determined without considering viscoelastic effects is highly questionable.…”

Section: Introductionmentioning

confidence: 99%

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Nanorheology of living cells measured by AFM-based force–distance curves

2020

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Mechanobiology aims to establish functional relationships between the mechanical state of a living a cell and its physiology. The acquisition of force-distance curves with an AFM is by far the dominant method to characterize the nanomechanical properties of living cells. However, theoretical simulations have shown that the contact mechanics models used to determine the Young's modulus from a force-distance curve could be off by a factor 5 from its expected value. The semi-quantitative character arises from the lack of a theory that integrates the AFM data, a realistic viscoelastic model of a cell and its finitethickness. Here, we develop a method to determine the mechanical response of a cell from a force-distance curve. The method incorporates bottom-effect corrections, a power-law rheology model and the deformation history of the cell. It transforms the experimental data into viscoelastic parameters of the cell as a function of the indentation frequency. The quantitative agreement obtained between the experiments performed on living fibroblast cells and the analytical theory supports the use of force-distance curves to measure the nanorheological properties of cells. † Electronic supplementary information (ESI) available: Complete derivation of the analytical expressions for PLR and KV models, coefficients for a conical tip, correspondence between velocities and equivalent indentation frequencies, statistical analysis of the experiments performed on NIH 3T3 fibroblasts. See

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Section: Mechanical Model Of a Living Cellmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanorheology of living cells measured by AFM-based force–distance curves

2020

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“…12,13 In mechanobiology, there is some evidence that supports the influence of the cell's viscoelastic processes in its physiology. [14][15][16][17] However, the interpretation of the AFM data in terms of the viscoelastic parameters of the sample is quite challenging. Among other issues, there is no established and generally accepted framework to measure the viscoelastic properties from AFM experiments.…”

Section: Introductionmentioning

confidence: 99%

Fast, quantitative and high resolution mapping of viscoelastic properties with bimodal AFM

2019

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“…This viscosity is mostly related to the compressive viscoelastic coefficient (also known as the Trouton coefficient) because indentation measurements involve application of compressive forces normal to the sample surface [30]. The analytical solution (eq.…”

Section: Simplest Spring-dashpot Combinationsmentioning

confidence: 99%

Viscoelasticity in simple indentation-cycle experiments: a computational study

Efremov

Kotova

Timashev

2020

Preprint

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Instrumented indentation has become an indispensable tool for quantitative analysis of the mechanical properties of soft polymers and biological samples at different length scales. These types of samples are known for their prominent viscoelastic behavior, and attempts to calculate such properties from the indentation data are constantly made. The simplest indentation experiment presents a cycle of approach (deepening into the sample) and retraction of the indenter, with the output of the force and indentation depth as functions of time and a force versus indentation dependency (force curve). The linear viscoelastic theory based on the elastic-viscoelastic correspondence principle might predict the shape of force curves based on the experimental conditions and underlying relaxation function of the sample. Here, we conducted a computational analysis based on this theory and studied how the force curves were affected by the indenter geometry, type of indentation (triangular or sinusoidal ramp), and the relaxation functions. The relaxation functions of both traditional and fractional viscoelastic models were considered. The curves obtained from the analytical solutions, numerical algorithm and finite element simulations matched each other well. Common trends for the curve-related parameters (apparent Young's modulus, normalized hysteresis area, and curve exponent) were revealed. Importantly, the apparent Young's modulus, obtained by fitting the approach curve to the elastic model, demonstrated a direct relation to the relaxation function for all the tested cases. The study will help researchers to verify which model is more appropriate for the sample description without extensive calculations from the basic curve parameters and their dependency on the indentation rate.

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Time-resolved nanomechanics of a single cell under the depolymerization of the cytoskeleton

Cited by 36 publications

References 57 publications

Nanorheology of living cells measured by AFM-based force–distance curves

Nanorheology of living cells measured by AFM-based force–distance curves

Fast, quantitative and high resolution mapping of viscoelastic properties with bimodal AFM

Viscoelasticity in simple indentation-cycle experiments: a computational study

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