Time-related changes in expression of collagen types I and III and of tenascin-C in rat bone mesenchymal stem cells under co-culture with ligament fibroblasts or uniaxial stretching

Zhang, Lei; Tran, Nguyen; Chen, Huaiqing; Kahn, Cyril J.F.; Marchal, Sophie; Groubatch, Frédérique; Wang, Xiong

doi:10.1007/s00441-007-0564-6

Cited by 36 publications

(21 citation statements)

References 33 publications

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“…Cell adhesion to ECM proteins through physical association with integrins is associated with intracellular signaling events and is critical for successful tissue regeneration (Grzesiak et al 1997). Mechanical force increases the gene expression for collagen I and III in cycle-stretched cells obtained from ligament, such as anterior cruciate ligament cells (Kim et al 2002) and bone-marrow-derived mesenchymal stroma cells (Zhang et al 2008). ECM also plays important functional roles in interacting with numerous growth factors and signaling molecules to regulate cellular events such as cell adhesion, proliferation, migration, survival, and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts

et al. 2011

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We have found that a water-soluble alkaline-digested form of eggshell membrane (ASESM) can provide an extracellular matrix (ECM) environment for human dermal fibroblast cells (HDF) in vitro. Avian eggshell membrane (ESM) has a fibrous-meshwork structure and has long been utilized as a Chinese medicine for recovery from burn injuries and wounds in Asian countries. Therefore, ESM is expected to provide an excellent natural material for biomedical use. However, such applications have been hampered by the insolubility of ESM proteins. We have used a recently developed artificial cell membrane biointerface, 2-methacryloyloxyethyl phosphorylcholine polymer (PMBN) to immobilize ASESM proteins. The surface shows a fibrous structure under the atomic force microscope, and adhesion of HDF to ASESM is ASESM-dose-dependent. Quantitative mRNA analysis has revealed that the expression of type III collagen, matrix metalloproteinase-2, and decorin mRNAs is more than two-fold higher when HDF come into contact with a lower dose ASESM proteins immobilized on PMBN surface. A particle-exclusion assay with fixed erythrocytes has visualized secreted water-binding molecules around the cells. Thus, HDF seems to possess an ECM environment on the newly designed PMBN-ASESM surface, and future applications of the ASESM-PMBN system for biomedical use should be of great interest.

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Section: Introductionmentioning

confidence: 99%

Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts

et al. 2011

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“…Mechanical stretch of tendons, tenocytes, and mesenchymal stem cells increases expression of scleraxis, as well as type I and III collagens (9,40,41,48). Mechanical stretch of cardiac fibroblasts induces increased expression of type I and III collagens, possibly via a mechanism that requires protein kinase C or tyrosine kinase activity, but the effect on scleraxis expression was not determined (6,8,28).…”

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confidence: 99%

Role of scleraxis in mechanical stretch-mediated regulation of cardiac myofibroblast phenotype

Roché

Nagalingam

Bagchi

et al. 2016

American Journal of Physiology-Cell Physiology

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-The phenotype conversion of fibroblasts to myofibroblasts plays a key role in the pathogenesis of cardiac fibrosis. Numerous triggers of this conversion process have been identified, including plating of cells on solid substrates, cytokines such as transforming growth factor-␤, and mechanical stretch; however, the underlying mechanisms remain incompletely defined. Recent studies from our laboratory revealed that the transcription factor scleraxis is a key regulator of cardiac fibroblast phenotype and extracellular matrix expression. Here we report that mechanical stretch induces type I collagen expression and morphological changes indicative of cardiac myofibroblast conversion, as well as scleraxis expression via activation of the scleraxis promoter. Scleraxis causes phenotypic changes similar to stretch, and the effect of stretch is attenuated in scleraxis null cells. Scleraxis was also sufficient to upregulate expression of vinculin and F-actin, to induce stress fiber and focal adhesion formation, and to attenuate both cell migration and proliferation, further evidence of scleraxis-mediated regulation of fibroblast to myofibroblast conversion. Together, these data confirm that scleraxis is sufficient to promote the myofibroblast phenotype and is a required effector of stretch-mediated conversion. Scleraxis may thus represent a potential target for the development of novel antifibrotic therapies aimed at inhibiting myofibroblast formation. transcription factor; cardiac fibroblast; stretch; migration; proliferation MYOFIBROBLASTS, AS THE ACTIVATED form of fibroblasts, are major mediators of tissue fibrosis in the heart, lungs, dermis, kidneys, and gastrointestinal tract (17,26,29,36,42). Excess deposition of fibrillar collagens in the extracellular matrix (ECM) of these tissues imparts increased stiffness and reduced organ function. Cardiac fibrosis entails a poor prognosis, negatively impacting both systolic and diastolic function and eventually leading to heart failure and death (43). There currently exists no treatment for the arrest or reversal of cardiac fibrosis (38). However, alteration of the myofibroblast phenotype may provide a novel means for the treatment and even reversal of fibrotic lesions in various tissue types (20,21,23,26,34,37,49).In response to myocardial injury, the release of damage factors [such as profibrotic transforming growth factor-␤ (TGF-␤)] and mechanical strain resulting from the loss of ECM integrity induce activation of cardiac fibroblasts and subsequent conversion to the myofibroblast phenotype (11,14,22,25,32). Cardiac myofibroblasts are characterized by hypersynthesis of fibrillar collagens type I and III, increased expression of ␣-smooth muscle actin (␣-SMA), increased adhesions and cell size, reduced proliferation and migration, and the formation of stress fibers (12,18,39,44,46). The morphological and functional changes that cardiac fibroblasts undergo during their conversion to myofibroblasts are critical to the wound healing process following myocardial injury. The loca...

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“…To date, the majority of current co-culture models can be categorized into two types: those which examine the effects of direct cell–cell contact on co-cultured cell populations (7–12) and those which focus on paracrine signaling and response to soluble signaling factors (13–17). These studies can be conducted at relatively macro- (18, 20, 21) or microscales (22–25).…”

Section: Introductionmentioning

confidence: 99%

Multifunction Co-culture Model for Evaluating Cell–Cell Interactions

Bogdanowicz

2013

Methods in Molecular Biology

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Interactions within the same cell population (homotypic) and between different cell types (heterotypic) are essential for tissue development, repair, and homeostasis. To elucidate the underlying mechanisms of these cellular interactions, co-culture models have been used extensively to investigate the role of cell–cell physical contact, autocrine and/or paracrine interactions on cell function, as well as stem cell differentiation. Specifically, the mixed co-culture model is often optimal for interpreting the effects of cell–cell contact on cellular behavior in vitro, while indirect co-culture can be used to study the effects of paracrine signaling on cell reactions. Additionally, cell–cell contact can be controlled by establishing physical barriers, which are used to regulate spatial and temporal cell distribution patterns in co-culture. In this chapter, we describe a method for forming a removable permeable divider for temporally and spatially controlling cellular interactions. This model can be used to study the impact of both cell–cell contact and paracrine signaling on the behavior of the mixed population as a whole and on the response of each subpopulation of cells in co-culture.

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Time-related changes in expression of collagen types I and III and of tenascin-C in rat bone mesenchymal stem cells under co-culture with ligament fibroblasts or uniaxial stretching

Cited by 36 publications

References 33 publications

Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts

Hydrolyzed eggshell membrane immobilized on phosphorylcholine polymer supplies extracellular matrix environment for human dermal fibroblasts

Role of scleraxis in mechanical stretch-mediated regulation of cardiac myofibroblast phenotype

Multifunction Co-culture Model for Evaluating Cell–Cell Interactions

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