Time of flight-secondary ion mass spectrometry analysis of protein adsorption on a polyvinylidene difluoride surface modified by ion irradiation

Okuji, S.; Kitazawa, Hideaki; Takeda, Yoshihiko

doi:10.1016/j.colsurfb.2016.08.029

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…In order to overcome this drawback, various strategies for the modification of membranes resulting in the hindrance of protein adsorption have been proposed. Among the common modification approaches are: the formation of organic coatings [ 10 ], ion irradiation [ 11 ], polydopamine-mediated atom transfer radical polymerization [ 12 ], and composite membrane fabrication [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

DLC-Coated Ferroelectric Membranes as Vascular Patches: Physico-Chemical Properties and Biocompatibility

et al. 2021

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In this paper, the results on the fabrication of ferroelectric membranes as vascular patches with modified surfaces are presented. For the modification of a membrane surface contacting blood, DLC coating was deposited using the pulsed vacuum arc deposition technique. The physico-chemical properties and cytotoxicity of the membranes modified under various conditions were studied. It was found that DLC coatings do not affect membrane microstructure, preserving its crystal structure as well as its high strength and elongation. It was revealed that an increase in the capacitor storage voltage results in the rise in sp2- and sp-hybridized carbon concentration, which makes it possible to control the chemical structure and surface energy of the modified surface. The experiments with 3T3L1 fibroblasts showed no toxic effects of the materials extracts.

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Section: Introductionmentioning

confidence: 99%

DLC-Coated Ferroelectric Membranes as Vascular Patches: Physico-Chemical Properties and Biocompatibility

et al. 2021

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“…More recently, there have been noteworthy reports about polar subsurface modifications that significantly affect the amount of adsorbed proteins 10 – 12 . A notably reduced adsorption of albumin was recently also found to occur above an amphiphilic subsurface gradient, i.e.…”

Section: Introductionmentioning

confidence: 99%

Nanoconfined water can orient and cause long-range dipolar interactions with biomolecules

Hegemann

Hocquard

Heuberger

2017

Sci Rep

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Surface properties are generally determined by the top most surface layer also defining how molecules adsorb onto it. By exploring effects due to interactions with deeper subsurface layers, however, long-range interaction forces were found to also significantly contribute to molecular adsorption, in which hydration of the subsurface region is the key factor. Water molecules confined to a subsurface amphiphilic gradient are confirmed to cause these long-range dipolar interactions by preferential orientation, thus significantly changing the way how a protein interacts with the surface. These findings imply future exploitation of an additional factor to modulate adsorption processes.

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“…Polyvinylidene difluoride (PVDF) membrane, a fluoropolymer, has a high mechanical strength and hydrophobicity, and has been widely adopted in biomedical applications, such as western blotting . Because of hydrophobicity, PVDF membrane usually need soak in carbinol for 30 s to become hydrophilic before transfer.…”

mentioning

confidence: 99%

Checking transfer efficiency and equal loading via qualitative optical way in western blotting

Gong

Zheng

2017

Electrophoresis

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The ability to determine that successful transfer and equal loading occur prior to using primary antibodies is important. And total protein staining is commonly used to check transfer efficiency and normalization, which play a crucial role in western blotting. Ponceau S and coomassie blue are commonly used, but there are disadvantages reported in recent years. Therefore, we are interested in finding another method, which is cheap, easy and fast. As we know, protein binding region of PVDF membrane is still hydrophilic when carbinol volatilizes, however, the non-protein binding region of PVDF membrane became hydrophobic again. And this different wettability between non-protein binding region and protein binding region of Polyvinylidene difluoride membrane may be used to check transfer efficiency and equal loading in western blotting. Based on the principle above, we describe an optical approach where an experimenter can observe that the proteins have been transferred to the membrane without any staining within minutes.

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Time of flight-secondary ion mass spectrometry analysis of protein adsorption on a polyvinylidene difluoride surface modified by ion irradiation

Cited by 4 publications

References 25 publications

DLC-Coated Ferroelectric Membranes as Vascular Patches: Physico-Chemical Properties and Biocompatibility

DLC-Coated Ferroelectric Membranes as Vascular Patches: Physico-Chemical Properties and Biocompatibility

Nanoconfined water can orient and cause long-range dipolar interactions with biomolecules

Checking transfer efficiency and equal loading via qualitative optical way in western blotting

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